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Cover Image
Cover Image
On the cover
A chemotaxing Dictyostelium cell (gray) forms stationary “traction adhesions” along its anterior-posterior axis that transmit force (indicated by heat map) to the underlying substrate and allow the cell to move. The colored cylinder shows the path of the migrating cell over time.
Image © 2014 Bastounis et al.
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In This Issue
In Focus
Motor proteins Hook on to early endosomes
Two studies identify a linker protein that coordinates endosome motility by recruiting microtubule-based motors.
People & Ideas
Yves Barral: Lessons from yeast on growth, renewal, and old age
Barral studies the regulation of cell growth and aging in yeast.
Review
Report
Cyclin B–Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase–independent mechanism
The role of Greatwall kinase in autoregulatory activation of cyclin B–Cdk1 at M phase onset can be bypassed by cyclin B–Cdk1–mediated direct phosphorylation of Arpp19, leading to PP2A-B55 inhibition.
Dual-mode regulation of the APC/C by CDK1 and MAPK controls meiosis I progression and fidelity
MAPK and Cdk1 play compensatory roles in suppressing APC/C activity early in prometaphase, thereby allowing accumulation of APC/C substrates essential for meiosis I.
Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint
Mad1 recruitment to metaphase kinetochores reactivates the mitotic checkpoint, which requires the C terminus of Mad1 in addition to its Mad2-binding domain.
Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy
The E3 ligase Ltn1 and the deubiquitylase Ubp3-Bre5 titrate the level of ribosomal subunit ubiquitylation and thereby set the rate of ribosomal protein degradation by ribophagy in response to nutrient supply and the level of protein translation.
The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission
OPA1 processing by YEM1L and OMA1 is dispensable for mitochondrial fusion and instead drives mitochondrial fragmentation, which is crucial for mitochondrial integrity and quality control.
Article
Oxidative stress–induced assembly of PML nuclear bodies controls sumoylation of partner proteins
PML multimerization into nuclear bodies following its oxidation promotes sumoylation and sequestration of partner proteins in these structures.
EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B
EB1 enables spindle microtubules to regulate the phosphorylation of kinetochores through recruitment of Aurora B kinase.
The kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization
The depolymerase activity of Kip3 suppresses spindle microtubule polymerization to limit spindle midzone length and prevent microtubule buckling in response to sliding forces.
Cytokinetic nodes in fission yeast arise from two distinct types of nodes that merge during interphase
Two distinct classes of cortical nodes form separately during interphase in fission yeast cells and then merge at the cell equator by a diffuse-and-capture mechanism to prepare nodes to form the contractile ring for cytokinesis.
Hook is an adapter that coordinates kinesin-3 and dynein cargo attachment on early endosomes
The Ustilago maydis Hook protein Hok1 is part of an evolutionarily conserved protein complex that regulates bidirectional early endosome trafficking by controlling attachment of both kinesin-3 and dynein.
HookA is a novel dynein–early endosome linker critical for cargo movement in vivo
HookA is a novel linker protein that binds to endosomes and to dynein–dynactin and promotes dynein–early endosome interaction in Aspergillus.
Bleb-driven chemotaxis of Dictyostelium cells
In Dictyostelium, mechanical resistance induces bleb-driven chemotactic movement that is controlled through PI3-kinase.
Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
During chemotactic movement, D. discoideum exhibits step-wise amoeboid motility driven by both contractile axial forces and lateral forces.
Kruppel-like factor 4 attenuates osteoblast formation, function, and cross talk with osteoclasts
KLF4 controls bone homeostasis by negatively regulating both osteoclast and osteoblast differentiation.
