Immunosenescence, or immune aging, is a hallmark of aging. This study shows that inhibition of insulin/IGF-1 receptor reverses immunosenescence in the roundworm Caenorhabditis elegans through a feedback circuit comprising FOXO, HSF-1, and bZIP transcription factors and an insulin-like peptide, INS-7.

Dünkler et al. discover that the subunit Pea2 recruits type V myosin Myo2 to the yeast polarisome. Experimental evidence and biophysical modeling indicate that this interaction generates an actin-dependent force to physically compact and thus spatially focus the polarisome and to mold the bud into its characteristic pointed shape.

Assembly of the destruction complex is critical for Wnt/β-catenin signaling. Nong et al. find that Axin undergoes liquid–liquid phase separation, which is promoted by APC. Phase-separated Axin provides a scaffold for recruitment of GSK3β, CK1α, and β-catenin and facilitates GSK3β-mediated β-catenin phosphorylation and thus degradation.

Fic et al. identify Drosophila RhoGAP19D as a novel epithelial polarity factor that excludes active Cdc42 from the lateral domain. Loss of RhoGAP19D causes apical domain expansion, lateral contractility, and invasion of adjacent tissues, a phenotype resembling that of precancerous breast lesions.

Rabanal-Ruiz and Byron et al. present a novel mechanism of nutrient signaling that identifies FAs as key cellular hubs that coordinate growth factor signaling and amino acid input into the cell and are required for efficient downstream activation of mTORC1.

Jones et al. determine the role of matrix stiffness in regulating chromatin accessibility in freshly isolated lung fibroblasts. They identify ZNF416 as a key transcriptional regulator controlling fibroblast activation by the mechanical environment with relevance to wound healing and fibrosis.

Restricting the localization of CENP-A to centromeres is essential to prevent chromosomal instability (CIN). CENP-A is overexpressed and mislocalized in several cancers. Shrestha et al. show that overexpression of CENP-A leads to its mislocalization, CIN, and aneuploidy with karyotypic heterogeneity.