Abir Elfarkouchi, Arthur Vitard, Léa Mammri, Sébastien Brier, Lola Tanneur, Cécile Hu, Aurélien Thureau, Nicolas Joly, Francoise Ochsenbein, Paul T. Conduit
Abir Elfarkouchi, Arthur Vitard et al. reveal the first structural model for CM1 domain autoinhibition, which prevents ectopic binding and activation of microtubule-nucleating γ-tubulin ring complexes. The model shows that intramolecular hairpin folding and species-specific domain associations sequester critical binding residues. In addition, they uncover key evolutionary divergences in how the CM1 binds γ-tubulin ring complexes.
Emma M. Sydir, M. Humam Farra, Abigail L. Whitford, Shea Hinojosa, Pei-Yi Kao, Joao A. Paulo, Sharan Swarup, C. Patrick Lusk, J. Wade Harper, I-Ju Lee, David Pellman
Sydir et al. identify an ESCRT-independent pathway for nuclear envelope assembly in S. japonicus that involves the conserved proteins, Vid27 and Alx1. This work sheds light on alternative nuclear envelope assembly processes that may explain why the Cmp7/ESCRT-III pathway is not essential in a number of organisms and cell types.
Zhao et al. develop constitutive and inducible Golgi reporter mice that enable in vivo visualization of Golgi architecture and Golgiphagy at single-cell resolution. Their study reveals tissue-specific Golgiphagy heterogeneity and stress-induced Golgi remodeling, providing a powerful platform to investigate Golgi homeostasis, disease mechanisms, and therapeutic opportunities.
Saroj Gourkanti, Gayathri Ramakrishnan, Jacqueline Cheung, Taylor J. Schoen, Yazmin Munoz, Rosa M. Chavez, Jan Dohnálek, Katie Martin, Matthew Lovett-Barron, Thomas Whisenant, Kvido Strisovsky, Sonya E. Neal
Gourkanti et al. show that the rhomboid protease Rhbdl2 restrains macrophage recruitment and wound repair in zebrafish. Loss of Rhbdl2 enhances macrophage accumulation and migration speed at wounds via elevated Rac2, accelerating tissue regeneration, positioning Rhbdl2 as a potential brake on macrophage-driven repair with relevance to inflammatory and fibrotic disease.
Jessica Sheu-Gruttadauria, Xiaowei Yan, Nico Stuurman, Riley O. Ogrean, Sabrina Lin, Stephen N. Floor, Ronald D. Vale
Sheu-Gruttadauria et al. show that the material properties of the nucleolus are shaped by the assembly state of ribosomal precursors, which tune nucleolar dynamics and morphology through their interactions with the nucleolar scaffolding protein NPM1.
Using computational protein–protein interaction screens, Vignogna and Fromme identify Avl9 as a GTPase-activating protein for Arf-family GTPases. They demonstrate that Avl9 and related DENN-domain proteins constitute a previously unrecognized group of GAPs, revealing unexpected functional diversity within this protein family.
Wenchao Qian, Hui Chen, Hongju Lee, Matthew C. Good
ZGA is a watershed event in early development essential for subsequent cell fate determination and morphogenesis. In this study, Qian et al. determined that proper spatial patterning of ZGA is required for embryogenesis and the existence of a surveillance system for embryo quality control exquisitely tuned to spatiotemporal ordering of genome activation.
Harriet P. Lo, Stéphane Vassilopoulos, Nicholas Ariotti, Thomas E. Hall, Robert G. Parton
Lo et al. provide a perspective on the role of skeletal muscle caveolae, focusing on the cellular processes impaired by caveola dysfunction in muscle disease. They propose a new classification for disease-associated CAV3 variants.
Geert Kops previews work from Tran and colleagues, which uses super resolution live imaging to show that condensin is required for local structural organization and microtubule attachment of kinetochores.
