Liu et al. identify WDR91 as an important player in retromer-dependent recycling. WDR91 promotes the interaction of Rab7 with SNX-retromer components and interacts with FAM21, facilitating the formation of the endosomal retrieval subdomain.

Lysosomal damage induces stress granule (SG) formation and translational reprograming. The newly appreciated process of atg8ylation affects SG formation and concomitantly recruits SG core proteins NUFIP2 and G3BP1 to damaged lysosomes. These proteins independently of SG condensates and in coordination with galectin-8 inactivate mTOR via the Ragulator–Rag complex.

This work demonstrates the lipid composition and the corresponding biophysical properties of biological membranes to tune highly specific protein–lipid interactions. Specifically, cholesterol is shown to affect phosphoinositide-dependent membrane recruitment and translocation into the extracellular space of FGF2, a survival factor involved in tumor-induced angiogenesis.

The biogenesis of secretory granules (SGs) is poorly understood. Here, we show that chromogranin proteins form a liquid scaffold upon entry into the milieu of the trans-Golgi network (TGN). These scaffolds recruit clients such as proinsulin and facilitate their transport to SGs.

Faklaris et al. present tools, fast and robust acquisition protocols, and automated analysis methods to assess quality control metrics for light fluorescence microscopy. The authors collected data from 10 light microscopy core facilities and propose guidelines to ensure quantifiable and reproducible results among laboratories.

Tubular recycling endosomes originate from early endosomes to deliver contents to target membranes. Jani et al. show that BLOC-1 and PI4P (synthesized by PI4KIIs) remodel membranes to generate and stabilize recycling tubules for cargo trafficking and exploitation by pathogens.

Contents are delivered to maturing melanosomes from early endosomal intermediates through tubular transport carriers. Zhu et al. show that two type II phosphatidylinositol kinases, PI4KIIα and PI4KIIβ, sequentially generate phosphatidylinositol-4-phosphate during tubule initiation and elongation for ultimate melanosome content delivery.