Mahone et al. use genetic, biochemical, and single-molecule imaging approaches to define a signaling pathway that coordinates chromosome segregation with progression of constriction during cell division in the bacterium Caulobacter crescentus. This pathway ensures genome integrity during the division process.
The dynamic recruitment of LAB proteins senses meiotic chromosome axis differentiation in C. elegans
Wang et al. explore the dynamic behaviors of two chromosome axis–associated proteins, LAB-1 and LAB-2, during meiotic prophase in Caenorhabditis elegans, revealing that their recruitment senses axis differentiation. Both proteins have phase separation capacities, which may promote the establishment of distinct chromosome subdomains required for accurate chromosome segregation.
Omi et al. identify phosphatidylserine (PS) synthesis as a key metabolic vulnerability in B cell receptor (BCR)-positive B cell lymphomas. Inhibition of PS synthesis causes an imbalanced phospholipid metabolism via membrane contact-based lipid transfer machinery, leading to aberrant BCR hyperactivation and ultimately cell death.
Paul et al. demonstrate that NAP1/AZI2 is a cell cycle protein that activates TBK1 during mitosis and regulates key mitotic and cytokinetic proteins to ensure accurate cell division.
Some integral membrane proteins can reach the plasma membrane in a Golgi-bypassing manner, a process also known as type IV UcPS. Here, Li et al. report that RAB-8– and RAB-11–positive endosomes are utilized as intermediate carriers in the unconventional apical protein transport.
An mTurq2-Col4a1 mouse model allows for live visualization of mammalian basement membrane development
Jones et al. describe a new mouse model for live visualization of the basement membrane (BM) in which endogenous type IV collagen is fluorescently labeled. Live imaging BM dynamics during skin development reveals that epidermal progenitors remain attached to and deform the BM during division, rather than lose adhesion as generally thought.