Extracellular vesicles (EVs) may mediate intercellular communication through the transfer of cargo molecules. Song et al. report that cyclin D1 is sorted into EVs during neuronal differentiation and that these EVs help to promote the neural induction of embryonic stem cells.

Frazer et al. use 4D imaging to reveal that while the intracellular pathways orchestrating killing within CTL are conserved, the rate of initiation along these pathways varies with TCR signal strength.

The assembly of the γ-secretase complex is coordinated by prior formation in the ER of dimeric subcomplexes, which next independently exit the ER. This mechanism could both prevent premature assembly and help the maintenance of stoichiometric levels of γ-secretase subunits during the assembly process.

This work dissects the role of different KIF1A tail domains in motor activity and cargo binding. Based on the results, the authors propose a mechanism for the regulation of KIF1A cargo transport in hippocampal neurons.

Trillet et al. report that the glycoprotein GP130 orchestrates the availability of the G protein–coupled receptor for apelin APLNR at the plasma membrane of glioblastoma stem-like cells, thereby modulating APLN signaling toward self-renewal. By modulating ELMOD1 expression, GP130 ultimately tunes ARF-mediated endovesicular internalization and recycling.

Larocque et al. describe how tumor protein D52-like proteins associate with intracellular nanovesicles. These small transport vesicles are shown to be involved in recycling α5β1 integrins during cell migration and invasion.

This work identifies endoplasmic reticulum subdomains, where the sterol synthesis and export machineries are coupled to sustain endocytosis in yeast mother cells, but not in daughters, where endocytosis and plasma membrane loading with accessible sterols are linked to secretion.