Liu et al. identify that the endothelium-derived fibronectin production regulated by Atg7 is required for the astrocytes attachment to microvascular wall. Endothelial Atg7 deficiency suppresses fibronectin via PKA/CREB signaling. This study uncovers the autophagy-independent function of endothelial Atg7 in the maintenance of the BBB integrity.

Macrophages activate the cytoprotective factor Nrf2 upon phagocytic ROS production via PI3K-calcium-Nox signaling during immune surveillance. This protective response is crucial to sustain vital immune functions and limit macrophage acquisition of senescence-like features. Macrophage Nrf2 also acts non-autonomously to limit bystander damage to adjacent healthy tissues.

Mascarau et al. demonstrate the relevance of HIV-1 infection of tissue-resident macrophages by fusion with infected CD4⁺ T cells and show that this process is modulated by the macrophage activation state and is under the control of the CD81/RhoA/Myosin axis.

Neuronal endoplasmic reticulum (ER) appears continuous throughout the cell. Its shape and continuity are influenced by ER-shaping proteins, mutations in which can cause distal axon degeneration in Hereditary Spastic Paraplegia (HSP). We therefore asked how loss of Rtnl1, a Drosophila ortholog of the human HSP gene RTN2 ...

Dynein-driven chromosome movement facilitates chromosome synapsis in prophase I of meiosis, which is essential for genetic exchange and completion of meiosis. Dynein-1 is recruited by KASH5 in the outer nuclear envelope. The authors show that KASH5 is an activating dynein adaptor and characterize the KASH5–dynein interaction.

Pachytene piRNA biogenesis is a hallmark of the germline, distinct from another wave of pre-pachytene piRNA biogenesis with regard to the lack of a secondary amplification process known as the Ping-pong cycle. However, the underlying molecular mechanism and the venue for the suppression of the Ping-pong cycle remain ...

Udi et al. present a new method for quantitative subcellular fractionation of a wide range of mammalian cells while maintaining nuclear integrity. This method provides a fast and reliable means to perform mass spectrometry and DeDuvian enrichment analyses for the different cellular compartments.