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Structural Biology 2023

A special collection of recent articles from JCB.
Cytoskeleton

Silva Martins…Mavrakis: Septin filaments mediate actin-membrane anchoring.
Cytoskeleton

Cannon…Gladfelter: Evolution of the biophysical properties of septins.

New Articles

Article

| March 29 2023

HIV infection of tissue macrophages by cell fusion

Rémi Mascarau, Marie Woottum, Léa Fromont, Rémi Gence, Vincent Cantaloube-Ferrieu, Zoï Vahlas, Kevin Lévêque, Florent Bertrand, Thomas Beunon, Arnaud Métais, Hicham El Costa, Nabila Jabrane-Ferrat, Yohan Gallois, Nicolas Guibert, Jean-Luc Davignon, Gilles Favre, Isabelle Maridonneau-Parini, Renaud Poincloux, Bernard Lagane, Serge Bénichou, Brigitte Raynaud-Messina, Christel Vérollet

Mascarau et al. demonstrate the relevance of HIV-1 infection of tissue-resident macrophages by fusion with infected CD4⁺ T cells and show that this process is modulated by the macrophage activation state and is under the control of the CD81/RhoA/Myosin axis.

Article

| March 23 2023

Presynaptic ER organization and Ca²⁺ dynamics

Juan José Pérez-Moreno, Rebecca C. Smith, Megan K. Oliva, Filomena Gallo, Shainy Ojha, Karin H. Müller, Cahir J. O’Kane

Neuronal endoplasmic reticulum (ER) appears continuous throughout the cell. Its shape and continuity are influenced by ER-shaping proteins, mutations in which can cause distal axon degeneration in Hereditary Spastic Paraplegia (HSP). We therefore asked how loss of Rtnl1, a Drosophila ortholog of the human HSP gene RTN2 ...

Article

| March 22 2023

KASH5 is an activating dynein adaptor

Kirsten E.L. Garner, Anna Salter, Clinton K. Lau, Manickam Gurusaran, Cécile M. Villemant, Elizabeth P. Granger, Gavin McNee, Philip G. Woodman, Owen R. Davies, Brian E. Burke, Victoria J. Allan

Dynein-driven chromosome movement facilitates chromosome synapsis in prophase I of meiosis, which is essential for genetic exchange and completion of meiosis. Dynein-1 is recruited by KASH5 in the outer nuclear envelope. The authors show that KASH5 is an activating dynein adaptor and characterize the KASH5–dynein interaction.

Article

| March 17 2023

The role of P-bodies in pachytene piRNA biogenesis

Mengneng Xiong, Lisha Yin, Yiqian Gui, Chunyu Lv, Xixiang Ma, Shuangshuang Guo, Yanqing Wu, Shenglei Feng, Xv Fan, Shumin Zhou, Lingjuan Wang, Yujiao Wen, Xiaoli Wang, Qingzhen Xie, Satoshi H. Namekawa, Shuiqiao Yuan

Pachytene piRNA biogenesis is a hallmark of the germline, distinct from another wave of pre-pachytene piRNA biogenesis with regard to the lack of a secondary amplification process known as the Ping-pong cycle. However, the underlying molecular mechanism and the venue for the suppression of the Ping-pong cycle remain ...

Tools

| March 15 2023

Mammalian cellular fractionation for proteomics

Yael Udi, Wenzhu Zhang, Milana E. Stein, Inna Ricardo-Lax, Hilda A. Pasolli, Brian T. Chait, Michael P. Rout

Udi et al. present a new method for quantitative subcellular fractionation of a wide range of mammalian cells while maintaining nuclear integrity. This method provides a fast and reliable means to perform mass spectrometry and DeDuvian enrichment analyses for the different cellular compartments.

Article

| March 15 2023

Exocyst's role in constitutive secretion

Conceição Pereira, Danièle Stalder, Georgina S.F. Anderson, Amber S. Shun-Shion, Jack Houghton, Robin Antrobus, Michael A. Chapman, Daniel J. Fazakerley, David C. Gershlick

Using kinetic trafficking assays, genetics, and secretomics, Pereira, Stalder et al. demonstrate the role of the exocyst complex in the secretory pathway. Exocyst subunits co-localize with post-Golgi carriers and their abrogation results in intracellular cargo accumulation in various cell types.

Article

| March 13 2023

Septins recruit transition zone to cilia

Darya Safavian, Moshe S. Kim, Hong Xie, Maha El-Zeiry, Oliva Palander, Lu Dai, Richard F. Collins, Carol Froese, Rachel Shannon, Koh-ichi Nagata, William S. Trimble

Safavian et al. show that septins and the associated RhoA GTP exchange factor ARHGEF18 regulate primary ciliogenesis by locally activating RhoA, leading to the activation of the exocyst complex and the subsequent recruitment of transition zone components.

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A special collection of recent articles from JCB.

Silva Martins…Mavrakis: Septin filaments mediate actin-membrane anchoring.

Cannon…Gladfelter: Evolution of the biophysical properties of septins.

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A special collection of recent articles from JCB.

Silva Martins…Mavrakis: Septin filaments mediate actin-membrane anchoring.

Cannon…Gladfelter: Evolution of the biophysical properties of septins.

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