Viol et al. show that the conserved Never-in-mitosis-A–related kinase-2 (Nek2) removes distal appendage components from the mother centriole prior to mitosis in every cell cycle. These findings suggest that Nek2 prevents cilia maintenance during mitosis via distal appendage regulation.

Cairo et al. show that in S. cerevisiae meiosis, spindle checkpoint proteins Bub1 and Bub3 have a critical role in preventing chromosome missegregation and setting the normal duration of anaphase I and II onset by regulating the kinetochore-localization of Ipl1 and PP1.

ALIX recruits ESCRT-III onto endosomes in a process that does not depend on other ESCRTs but requires the late endosome–specific lipid LBPA in vivo and in vitro. This novel pathway of ILV formation promotes tetraspanin sorting and delivery to exosomes.

Centrosomes withstand microtubule-mediated forces during spindle assembly, yet they are disassembled by similar forces during mitotic exit. Mittasch et al. use nanorheology to probe the material properties of centrosomes and how they change during the cell cycle. In anaphase, the centrosome scaffold becomes weak and brittle, thus allowing force-induced disassembly.

Yang et al. demonstrated TORC1 inactivation not only up-regulates macroautophagy and vacuole recycling but also leads to the degradation of many vacuole membrane proteins. The degradation is initiated by ubiquitination and followed by ESCRT-dependent microautophagy. Furthermore, TORC1 directly regulates the activity of vacuole ubiquitination machinery.

Ma et al. describe a novel retrograde trafficking pathway in which type II PI 4-kinase (PI4KII), Past1/EHD1, and Syntaxin-16 are needed for accumulation of PI4P on regulated secretory granules and for the tetraspanin CD63 to promote granule maturation.

Local mRNA translation in neurons at a distance from the nucleus is key to new synapse formation. Titlow et al. find that the Syncrip RNA binding protein acts directly on msp300 mRNA to modulate activity-dependent synaptic plasticity. Single-molecule imaging reveals an activity-dependent increase in mRNP complex size and colocalization with ribosomes and the translation initiation factor eIF4E at the synapse.