Hugo Muñoz-Hernández, Yixin Xu, Aitor Pellicer Camardiel, Daniel Zhang, Allen Xue, Amol Aher, Ellie Walker, Florina Marxer, Tarun M. Kapoor, Michal Wieczorek
The γ-TuRC is recruited to microtubule-organizing centers by NEDD1. Muñoz-Hernández et al. report cryo-EM structures revealing how NEDD1’s C terminus forms an α-helical tetramer that docks onto the γ-TuRC without inducing major conformational changes in the complex, compatible with CDK5RAP2 binding and γ-tubulin ring closure.
Claudia Pachinger, Jeroen Dobbelaere, Cornelia Rumpf-Kienzl, Shiviya Raina, Júlia Garcia-Baucells, Marina Sarantseva, Andrea Brauneis, Alexander Dammermann
Centriolar satellites are cytoplasmic particles found concentrated near centrosomes and the base of cilia in vertebrate cells whose functional contribution has long been unclear. Here, Pachinger et al. present a model for satellites not as transport modules but as sites of translation specifically of centrosomal and ciliary proteins.
Hye Jee Hahn, Natalya Pashkova, Michael A. Cianfrocco, Lois S. Weisman
This study provides insights into how cargo adaptors bind myosin V. Genetics, cell-based assays, cryo-EM, and AlphaFold reveal that the vacuole-specific adaptor uses a handhold mechanism to attach to two areas on the myosin-V tail. Moreover, evidence is presented that other adaptors use a similar strategy.
Justin Krish Williams, Jordan Matthew Ngo, Abinayaa Murugupandiyan, Dorothy E. Croall, H. Criss Hartzell, Randy Schekman
Human cells repair plasma membrane lesions in a two-step process. After a membrane scab forms at the lesion site, the scab is shed as annexin-containing extracellular vesicles.
Shun-ichi Yamashita, Ritsuko Arai, Hiroshi Hada, Benjamin Scott Padman, Michael Lazarou, David C. Chan, Tomotake Kanki, Satoshi Waguri
The precise roles of the mitophagy receptors BNIP3 and NIX remain unclear. This study demonstrates that they are required for tight attachment and expansion of the isolation membrane along the mitochondrial surface, supporting a characteristic morphologic feature of the mitophagy.
Fiona H. Murphy, Adlin Abramian, Remco V. Klaassen, Frank Koopmans, Claudia M. Persoon, August B. Smit, Ruud F. Toonen, Matthijs Verhage
This study examines how RIM and MUNC13 synergistically regulate DCV exocytosis. It demonstrates that MUNC13 is essential for DCV secretion, while RIM prevents its degradation. Key membrane-binding domains of RIM and MUNC13 play differing roles, revealing distinct mechanisms from synaptic vesicle release.
Lieke Stockmann, Hélène Kabbech, Gert-Jan Kremers, Brent van Herk, Bas Dille, Mirjam van den Hout, Wilfred F.J. van IJcken, Dick H.W. Dekkers, Jeroen A.A. Demmers, Ihor Smal, Danny Huylebroeck, Sreya Basu, Niels Galjart
In mouse embryonic stem cells, the kinesin-13 member KIF2A converts from a microtubule depolymerase during metaphase to a microtubule stabilizer during cytokinesis, due to the inhibition of ATPase activity and a preference for compacted lattices. KIF2A-mediated microtubule stabilization prolongs cytokinesis to maintain stem cell pluripotency.
