How lipid droplets are formed is largely unclear. Chen et al. show that FIT2 recruits ER tubule-forming proteins and septin cytoskeletons to facilitate nascent LD formation by providing membrane curvature and bulging scaffold.

May et al. combine proximity labeling with quantitative mass spectrometry to profile the proteome of primary cilia in cells responding to Hedgehog. They identify signaling factors that undergo dynamic ciliary enrichment and uncover an unanticipated regulation of ciliary cAMP signaling.

Doodhi et al. reconstitute the yeast kinetochore–microtubule interface in vitro and compare the relative strength of distinct kinetochore–microtubule interaction modes. Their results suggest how Aurora B kinase promotes the exchange of kinetochore–microtubule interactions to eliminate aberrant interactions and establish chromosome biorientation.

Vessoni et al. demonstrate telomere shortening leads to a unique DDR response in human pluripotent stem cells. Unlike terminally differentiated cells, telomere shortening induces formation of single-stranded DNA telomere overhangs in hPSCs, which activate ATR signaling and lead to mitotic catastrophe and p53-dependent cell death.

Oshima et al. report that in the absence of Parkin activity, cells adapt to mitochondrial proteotoxicity through a Drp1-mediated mechanism that includes the severing of the OMM and IMM ubiquitination, followed by recruitment of autophagy machinery to the ubiquitinated IMM proteins.

Live-cell microscopy reveals a novel mode of function for COPII: COPII binds the membrane at the boundary between the ER and ER exit sites, where it executes cargo sorting and concentration into ERES and does not coat cargo exporting containers.

Yan et al. identify C. elegans RTKN-1 as a novel endocytic recycling regulator that impedes UNC-60A/cofilin-mediated endosomal actin disassembly. Further evidence indicates that the self-binding capacity of RTKN-1 is indispensable for this functionality and SDPN-1/Syndapin acts to direct the proper residency of RTKN-1 in recycling endosomes.