Mochida et al. find that the autophagy receptor Atg39 links the outer and inner nuclear membranes via two distinct membrane-binding regions. Autophagosome formation-coupled Atg39 condensation causes local nuclear envelope protrusion, leading to sequestration of a small piece of the nucleus into the autophagosome.

Drosophila tracheal terminal cells form subcellular tubes by invaginating their apical plasma membrane. Ríos-Barrera and Leptin show that late endosomes guide subcellular tube elongation toward the direction of cell growth by promoting F-actin organization between the tip of the subcellular tube and the advancing tip of the cell.

Kinoshita et al. address the role of condensin I in chromosome assembly and identify two classes of mutations that cause hyper- and hypocompaction phenotypes in Xenopus egg extracts. The study reveals that a loop extrusion–independent mechanism also contributes to chromosome assembly and shaping.

Addicks et al. identify a role for protein acetylation in the regulation of muscle integrity by linking GCN5 acetyltransferase activity to the expression of dystrophin and other genes important for muscle structure. They find that this occurs through GCN5-directed acetylation of the transcriptional repressor YY1.

Keen et al. discover a new role for eIF6, a protein classically known for its role in protein synthesis, in mechanotransduction, and the cellular response to force. These data provide a novel paradigm for how the cytoskeletal and translational machineries are linked.

SidK is introduced as a highly specific probe to visualize and quantify the proton-pumping vacuolar H+ATPases (V-ATPases) of mammalian cells and yeast.

Kors et al. reveal that peroxisome–ER associations via the ACBD5-VAPB tether are regulated by phosphorylation and GSK3β in mammalian cells. Phosphorylation sites in the FFAT-like motif of ACBD5 affect the binding to VAPB—and thus peroxisome–ER contact sites—differently.