Boutry et al. show that Arf1-PI4KIIIβ positive vesicles participate in the fission of lysosomal tubules through lysosomal PI(3)P regulation. This work identifies new components involved in the scission of lysosomal tubules and demonstrates that this process requires an exquisite regulation of phosphoinositides on lysosomes.

The ultrafast camera with single fluorescent-molecule sensitivities developed by Fujiwara et al. has greatly improved the time resolution of single-molecule localization microscopy, revealing the focal adhesion’s dynamic nano-architecture and leading to the model of compartmentalized archipelago of focal-adhesion protein islands.

An ultrafast camera developed by Fujiwara et al. allows single fluorescent-molecule imaging every 33 µs with a localization precision of 34 nm (every 100 µs; 20 nm) and enables ultrafast PALM imaging of whole live cells.

Illukkumbura et al. reveal that cluster-dependent tuning of membrane binding rather than size-dependent coupling to the actomyosin cortex enables differential transport of PAR proteins by cortical flows in the C. elegans zygote.

The mechanism of how mitotic kinetochores accomplish robust, long-distance coupling with spindle microtubule plus-ends during metaphase and anaphase is unknown. This work demonstrates that the replication licensing protein Cdt1 synergizes with kinetochore Ndc80 and the Ska1 complexes to form a tripartite Ndc80-Cdt1-Ska1 complex that processively tracks the plus-ends of dynamic microtubules.

Hakanpää et al. show that reticular adhesions are assembled at flat clathrin lattices, a process inhibited by fibronectin and its receptor, integrin α5β1. This novel adhesion assembly mechanism is coupled to cell migration and reveals a unique crosstalk between cell-matrix adhesions.

Gáspár et al. reveal that sequential activity of dynein and kinesin-1 motors during transport of oskar mRNA to the posterior of the Drosophila oocyte is orchestrated by competition between two double-stranded RNA-binding proteins, Egl and Staufen, and that this process is spatio-temporally controlled by dynein-mediated localization of the staufen mRNA.