Keen et al. discover a new role for eIF6, a protein classically known for its role in protein synthesis, in mechanotransduction, and the cellular response to force. These data provide a novel paradigm for how the cytoskeletal and translational machineries are linked.

Addicks et al. identify a role for protein acetylation in the regulation of muscle integrity by linking GCN5 acetyltransferase activity to the expression of dystrophin and other genes important for muscle structure. They find that this occurs through GCN5-directed acetylation of the transcriptional repressor YY1.

SidK is introduced as a highly specific probe to visualize and quantify the proton-pumping vacuolar H+ATPases (V-ATPases) of mammalian cells and yeast.

Kors et al. reveal that peroxisome–ER associations via the ACBD5-VAPB tether are regulated by phosphorylation and GSK3β in mammalian cells. Phosphorylation sites in the FFAT-like motif of ACBD5 affect the binding to VAPB—and thus peroxisome–ER contact sites—differently.

Krishnan et al. use FISH to show that specific forms of RB induce changes in the organization of euchromatin and heterochromatin domains. These changes are visible under the microscope, occur after cell cycle arrest, are separable from senescence, and represent an E2F-independent activity of RB.

Glycosylphosphatidylinositol (GPI) anchors attach many proteins to the extracellular surface of the plasma membrane. Anchor synthesis occurs in the ER and requires phosphatidylethanolamine (PE). Toulmay et al. show that tubelike lipid transport proteins bring PE to the ER to support GPI anchor production.

Hirsch et al. found that genetic disruption of central spindle microtubule assembly in C. elegans embryos does not block cytokinesis or midbody assembly. These central spindle–independent midbodies appeared to form from astral microtubules, bundled by contractile ring constriction.