Sandhya Srinivasan, William Ramos-Lewis, Mychel R.P.T. Morais, Qiuyi Chi, Adam W.J. Soh, Emily Williams, Rachel Lennon, David R. Sherwood
Srinivasan et al. construct a collagen IV fluorophore knock-in toolkit in C. elegans using a newly identified permissive genome-editing site and reveal tissue-specific trimer diversity and basement membrane turnover defects in collagen IV mutants modeling human COL4A1/A2 (Gould) syndrome.
Jiasheng Li, Jimeng Cui, Xinyu Li, Di Zhu, Zhenhua Chen, Xiahe Huang, Yingchun Wang, Qingfeng Wu, Ye Tian
Li et al. reveal that neuronal mitochondrial stress induces subtle, continuous Ca2+ oscillations in a TMBIM-2-dependent manner, enhancing neurotransmission and influencing intertissue mitochondrial stress communication. Restoring Ca2+ balance provides a strategy to improve neuronal function and metabolic regulation during aging.
Julia Schweighofer, Bhagyashree Mulay, Ingrid Hoffmann, Doro Vogt, Marion E. Pesenti, Andrea Musacchio
FAcilitates Chromatin Transcription (FACT), which stabilizes chromatin during transcription and replication, is mysteriously enriched at mitotic kinetochores. In this paper, direct interactions between FACT and the 16-subunit constitutive centromere–associated network core of the chromatin-proximal kinetochore are reconstituted and reciprocal requirements for their stability are identified.
Lauren E. Vostal, Noa E. Dahan, Matthew J. Reynolds, Lily I. Kronenberg, Tarun M. Kapoor
Vostal et al. report two structures of complexes formed by valosin-containing protein (VCP), an unfoldase, and VCPIP1, a deubiquitinase. These data, along with biochemical analyses, suggest a model in which VCPIP1 is poised to cleave ubiquitin from protein substrates that translocate through VCP’s central pore.
This study reveals the mechanism for yeast SAPK Hog1 activation by acetic acid stress. Stress granules induced by acetic acid exposure function as a scaffold to bring Hog1 together with its activating kinase Pbs2 without stimulation of the canonical HOG pathway beyond basal levels.
Gabrielle L. Turvey, Ernesto López de Alba, Emma Stewart, Heather Cook, Ahmad Alalti, Richard T. Gawne, Justin F.-X. Ainscough, Andrew S. Mason, Dawn Coverley
This paper shows that protein fragments derived from the CIZ1 gene disperse nuclear CIZ1-RNA assemblies, exposing underly loci and changing gene expression. The interference mechanism is implicated as a driver of epigenetic instability in early-stage breast cancers.
Yuchieh Jay Lin, Li-Ting Huang, Po-Yuan Ke, Guang-Chao Chen
Lysosomal acidification is crucial for cellular homeostasis, with impairment linked to aging and neurodegenerative diseases. We identify USP45 as a negative regulator of autophagy and lysosomal function, inhibiting V-ATPase localization to autolysosomes. USP45 regulates V-ATPase translocation via Coronin 1B–mediated actin modulation, suggesting therapeutic potential.
Sinead Iduna Schwabl, Konstantin Adrian Siegmann, David Teis
Schwabl et al. discuss work from Gao et al. demonstrating that the endosomal scramblase Any1 coordinates membrane remodeling and lipid transfer via Vps13 to drive multivesicular body biogenesis.
Velez-Aguilera and Jantsch discuss work from the Dumont lab that utilized sophisticated imaging approaches of Caenorhabditis elegans embryos undergoing meiosis to reveal the existence of a novel double-membrane structure, called the interkinetic envelope, in oocytes during interkinesis.
