Loi et al. introduce 3D-Speckler, a powerful and high-throughput image analysis software with a user-friendly interface that offers semi-automated, multi-dimensional measurements and corrections of fluorescent particles including 2D/3D size, localization with nanometer accuracy, and intensity for routine microscope calibration and quantitative analyses of biological structures.

Bebelman and Bovyn et al. demonstrate that apical bulkheads, connections between the apical membranes of opposing hepatocytes, are load-bearing mechanical elements that contribute to the ability of bile canaliculi to withstand elevated luminal pressure.

Du et al. show that Tex2, a potential lipid transporter on the tubular endoplasmic reticulum, is recruited to late endosomes/lysosomes by endosome-resident phosphatidylinositol 4-phosphatases TMEM55 to regulate the lipid compositions, trafficking, and the functions of lysosomes.

Cieslinski and Wu et al. employ super-resolution microscopy to obtain a comprehensive model of the kinetochore structure in budding yeast. By measuring precise positions and stoichiometries, they could resolve the arrangement of the major kinetochore subunits when under attachment tension during metaphase.

Virant et al. build an in situ model of the Schizosaccharomyces pombe kinetochore by determining inter-protein distances and copy numbers. Next to confirming its conserved nature known from in vitro data, they point out S. pombe specifics, e.g., within DASHc and the inner kinetochore structure.

Berman et al. examine the capping activity of the γ-TuRC. Using in vitro and cellular assays, they characterize how the γ-TuRC's nucleotide-independent capping activity regulates microtubule dynamics and how it contributes to microtubule formation during cell division.

Neurite pruning, a critical neuronal circuit specification mechanism, often involves neurite abscission by unknown mechanisms. In Drosophila sensory neurons, pruning dendrites first become mechanically fragile, and severing occurs through morphogenetic tissue movements that exert mechanical forces on the destabilized dendrites, thus establishing mechanical force as a mechanism during neurite pruning.