Yadav & Jadhav et al. present the design, synthesis, and validation of a novel cell-permeable biotin-HaloTag ligand. The reported ligand expands the HaloTag toolbox by enabling in vivo labeling and providing capabilities for efficient affinity capture of protein variants from live cells.

The WAVE complex is an actin nucleator that regulates sheet-like lamellipodial protrusions. M. Wu et al. find that the WAVE complex is a core and limiting component of a highly stereotyped membrane curvature-dependent linear array that could serve as a structural template for lamellipodial generation.

Naughton et al. establish that the ER experiences forces in the piconewton range across the ER lumen and identify NOMO as a force-bearing transmembrane protein that is required for myogenesis.

Neurons and muscles must maintain structural contact despite mechanical strain. Ermanoska et al. identify a presynaptic actomyosin core at Drosophila neuromuscular junctions that coordinates with integrin adhesion receptors to sense and respond to tension changes, preserving neuron–muscle mechanical continuity.

Teixeira et al. identify the onconeural antigen CDR2 as an ER-localized adaptor for the microtubule-based motor cytoplasmic dynein-1 and show that CDR2 contributes to ER organization through its interaction with the integral ER membrane protein kinectin.

Pasolli, Meiring et al. describe tools to acutely relocate vimentin intermediate filaments by inducibly coupling them to microtubule motors. Rapid perinuclear clustering of vimentin had no strong effects on microtubules or actin but was accompanied by redistribution of mitochondria and endoplasmic reticulum sheets, and reduced cell stiffness.

The authors identify the conserved unconventional class XV myosin, Myo15, an actin motor, as a novel regulator of presynaptic assembly and remodeling in Drosophila. They find Myo15 to be essential for presynaptic functional homeostatic plasticity and memory consolidation.