Quantitative assessments using single-molecule imaging and super-resolution microscopy revealed that all extracellular vesicle subtypes derived from four distinct tumor cell lines, regardless of size, bind to laminin predominantly via CD151-facilitated integrin heterodimers and GM1, leading to response of recipient cells.

Cytokinesis is tightly regulated in metazoans. Adhikary et al. show that LIN-5/GPR-1/2 complexes present at the polar and lateral regions of the cell membrane facilitate the accumulation of contractile ring components at furrow formation and stabilize them in the midbody, thus aiding in various cytokinesis processes.

Kinesin-1 alternately moves its two motor domains (heads) to walk along the microtubule. Makino et al. demonstrate that the detached head cannot rebind to the rear binding site because of an intolerable increase in tension, thus ensuring forward stepping after ATP binding.

This work introduces a novel and innovative tagged Col4a1 mouse model for studies of basement membrane dynamics. The authors elucidate important details of COL4A1 turnover in cerebral vasculature and demonstrate that Col4a1 heterozygous deletion is better tolerated than missense mutations.

Death of dopaminergic neurons in the brain is a hallmark of Parkinson’s disease. Gregori and colleagues show that deviant calcium signals in familial disease models caused by mutations in LRRK2 can be reversed by targeting lysosomal TPC2 channels and that TPC2 mimics disease effects in vivo.

Peng Huang et al. find that the ciliary kinesin OSM-3 exhibits an extended, active conformation at the ciliary base and middle segments. Their results indicate the NEK family kinases and PP2A phosphatase collaborate through a phosphorylation-mediated mechanism to regulate the regional motility of ciliary kinesin.

Nita et al. show that the FGFR2 localizes to primary cilia of the developing mouse tissues and in vitro cells; they identify the molecular mechanism of this action, and show its critical function in FGFR2 signaling.