How lysosomes change their numbers for cell division is unknown. Yin et al. reveal that lysosome numbers increase in the S and G2/M phases in the cell cycle and the cyclin-dependent kinases CDK4/6 regulate lysosome biogenesis through TFEB/TFE3.

The centralspindlin complex, comprising Tumbleweed RhoGAP and kinesin-like Pavarotti proteins, associates with microtubules during cytokinesis. Nakamura et al. show that Pavarotti has centralspindlin complex–independent functions, binds directly to actin, and regulates actin dynamics during cell wound repair and oogenesis.

The tumor suppressor PTEN is essential for epithelial morphogenesis. Qi et al. identify Abi1, a core adaptor protein in the WAVE regulatory complex, as a new PTEN substrate. PTEN dephosphorylates Abi1 and causes Abi1 degradation through calpains and thus down-regulates the WAVE regulatory complex to induce epithelial differentiation and polarization.

Chan et al. show that natural killer (NK) cells can be reprogrammed by breast cancer cells to promote metastasis. Reprogramming can be blocked by targeting NK cell inhibitory receptors TIGIT or KLRG1 or inhibiting DNA methyltransferases, which suggests new approaches to prevent or treat metastasis.

Chen et al. identified a bipartite motif from the 440-kD giant ankyrin-B, which bundles and avidly binds to microtubules in vitro. They further demonstrated that giant ankyrin-B suppresses axon collateral branching and prevents microtubule invasion of nascent axon branches through direct interaction with microtubules.

A single alternatively spliced exon in the CLTC gene contributes to clathrin coat organization. This event participates in the plasticity from clathrin-coated pits to plaques, structures that are essential for muscle fiber function and maintenance.

Jiang and colleagues investigate how locations of sites of organelle assembly are determined during cell division in the ciliate Tetrahymena thermophila. They find that correct placement of new organelles involves antagonistic influences of a cyclin E and a Hippo/Mst kinase.