Tey et al. characterize the role of ubiquitin in the rapid turnover kinetics of the immune checkpoint protein CTLA4. A complex architecture of associated ubiquitin chains is revealed by a comprehensive ubiquitin chain restriction analysis in the absence of the deubiquitylase USP8.

Liu et al. developed an optimized method to study transcriptional dynamics in living C. elegans. They inserted MS2 loops in the intron of mCherry and tagged endogenous genes with this modified mCherry, which allowed monitoring transcription upon stress and avoided interfering with endogenous gene expression.

Fahim and Marcus et al. developed live-cell microscopy approaches to address whether co-localized enzyme factors are forming biochemically relevant interactions within RNA–protein condensates. These tools enable rigorous tracking of protein–protein interactions within biomolecular condensates, such as stress granules and mRNA processing bodies.

de Jager et al. show that compacted and expanded microtubule lattice spacings coexist within cells and that microtubules recognized by a marker for stable microtubules are predominantly expanded. This adds another potential regulatory layer to the organization of microtubule networks and the establishment of specialized subsets.

Ishikawa et al. establish a comprehensive resource of strains and plasmids to suppress genes required for fission yeast viability. Strains and plasmids are individually stored, and they enable complicated phenotypical analyses, facilitating functional investigations of essential genes that are evolutionary conserved among eukaryotes.

While previous work suggested that Dachsous promotes growth via the Hippo pathway and Fat represses it, Matakatsu and Fehon show that these proteins function together to restrict growth by coordinately removing a “core complex” consisting of Dachs, Dlish, and Approximated from the junctional cortex.

Du et al. identify Sec23IP as an adaptor that recruits VPS13B to ER exit site–Golgi interfaces, and the interaction is required for the formation of tubular ERGIC and efficient ER export of procollagen. These findings reveal a role of VPS13B–Sec23IP interaction in Cohen syndrome pathogenesis.