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Yu-Cheng Zhang, Yun-Feng Bai, Jin-Feng Yuan, Xiao-Lin Shen, Yu-Ling Xu, Xiao-Xiao Jian, Sen Li, Zeng-Qing Song, Huai-Bin Hu, Pei-Yao Li, Hai-Qing Tu, Qiu-Ying Han, Na Wang, Ai-Ling Li, Xue-Min Zhang, Min Wu, Tao Zhou, Hui-Yan Li
Zhang et al. identify CEP55 as a novel regulator of cilia disassembly. Their findings establish a cilia disassembly role for CEP55 in vivo and in vitro, coupling defects in cilia disassembly to ciliopathy and further suggesting that proper cilia dynamics are critical for mammalian development.
Kimberly K. Fong, Trisha N. Davis, Charles L. Asbury
Microtubule attachments to spindle poles in yeast are flexible, which allows the microtubules to pivot. Fong et al. directly measure the pivoting flexibility of the microtubule–pole interface and show that this flexibility is important for timely pole separation during mitosis.
David J. Thaller, Danqing Tong, Christopher J. Marklew, Nicholas R. Ader, Philip J. Mannino, Sapan Borah, Megan C. King, Barbara Ciani, C. Patrick Lusk
Thaller et al. demonstrate that direct binding between phosphatidic acid (PA) and the ESCRT Chm7 is required for nuclear envelope surveillance; PA also accumulates at nuclear envelope herniations. Thus, tight control of PA metabolism is required for nuclear envelope homeostasis.
Gil Kanfer, Shireen A. Sarraf, Yaakov Maman, Heather Baldwin, Eunice Dominguez-Martin, Kory R. Johnson, Michael E. Ward, Martin Kampmann, Jennifer Lippincott-Schwartz, Richard J. Youle
Kanfer et al. develop a pooled CRISPRi screening method to identify genes regulating intracellular protein localization, organelle morphology or other subcellular phenotypes. The method uses machine learning to identify genetically altered cells, a photoactivated fluorescent protein to label them, and FACS plus deep sequencing to identify the affected gene.
Xiaowei Yan, Nico Stuurman, Susana A. Ribeiro, Marvin E. Tanenbaum, Max A. Horlbeck, Christina R. Liem, Marco Jost, Jonathan S. Weissman, Ronald D. Vale
Yan et al. demonstrate high-throughput screening of pooled CRISPR libraries for phenotypes detectable by microscopy. Their approach uses photoactivation of cells displaying the phenotype of interest and FACS sorting of marked cells, followed by sequencing, and facilitates discovery of genes involved in cell biological processes.
Jennifer Hirst, Geoffrey G. Hesketh, Anne-Claude Gingras, Margaret S. Robinson
The AP-5/SPG11/SPG15 complex is recruited onto late endosomes/lysosomes and contributes to lysosomal homeostasis and autophagic lysosome reformation. Hirst et al. show that recruitment is by coincidence detection, requiring both phosphatidylinositol 3-phosphate and Rag GTPases, thus uncovering a link between AP-5/SPG11/SPG15 and the mTORC1 pathway.
Amr H. Allam, Mirren Charnley, Kim Pham, Sarah M. Russell
During T cell development, genomic rearrangement must create a T cell receptor capable of transmitting signals. Allam et al. show that passage through the β-selection checkpoint requires the assembly of a platform to support TCR signaling, similar to the mature T cell immunological synapse.

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Current Issue
Volume 220,
Issue 1,
January 4, 2021
Reviews & Opinions
People & Ideas
Melina Casadio, Dan Simon
JCB checks in with newly independent cell biologists and learns about their experience running a lab during the COVID-19 pandemic.
Mary Catherine Bridges, Amanda C. Daulagala, Antonis Kourtidis
Bridges et al. discuss current knowledge, lingering questions, and emerging themes surrounding lncRNA subcellular localization and its impact on cellular functions.
Simon Latour, Alison P. McGuigan
Latour and McGuigan highlight work from the Derivery laboratory that describes a new method for micropatterning proteins while maintaining their activity using fibrinogen anchors.

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