This study shows that the combination of hypoxia and acidosis, common in tumors, produces only transient activation of HIF-1α activity due to its autophagic degradation. Consequently, key HIF targets are not induced, helping prevent excessive acidification by fermentation. This pH-hypoxia interplay has implications for cancer diagnostics, treatment, and experimental design.

Jung and Kim et al. show that combined ALDH9A1 and FANCD2 deficiency leads to genomic instability, decreased cell survival, and tumorigenesis. Loss of ATP13A3, a polyamine transporter, rescues cellular growth, implicating aminoaldehydes in the creation of DNA damage, which necessitates repair by the Fanconi anemia pathway.

Morgan et al. demonstrate that pan-expansion microscopy is an exceptional tool for probing the molecular composition and structural plasticity of individual NPCs: they reveal LINC complex–dependent NPC diameter biases across the nuclear surface and changing nucleoporin positions in an ALS model.

Kim et al. report on Arc, a large PDZ domain–containing cell membrane–associated protein that controls Drosophila salivary gland architecture. Arc facilitates apical membrane delivery of Crumbs; in turn, Crumbs limits accumulation and activity of non-muscle myosin II to regulate the number of salivary gland cells that internalize at a given time.

This study reveals that the kinase LKB1 is crucial for migration and nuclear positioning of specialized type of RGCs called F-RGCs. Its absence leads to misplacement, altered morphology, and increased cell death, highlighting that different RGC types can use distinct migration programs.

Studies described in this manuscript show that cyclin N-terminal domain containing 1 (CNTD1) is a critical regulator of crossover formation during prophase I of meiosis in females. Loss of CNTD1 results in severe depletion of oocytes at birth leading to loss of the ovarian reserve.

Breast cancer cells isolated from lung micrometastases have altered metabolism, which influences extracellular vesicle production to generate invasive microenvironments.