Journal of Cell Biology (JCB) | Rockefeller University Press

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Tools Collection 2023

A special collection of recent methods and resources from JCB.
Membrane and lipid biology

Brukman…Podbilewicz: IZUMO1 is a sperm fusogen.
Cell death and autophagy

Zhou…Dengjel: Degradation of TNIP1 by selective autophagy.

New Articles

Article

| February 03 2023

Remodeling of the clathrin coat during endocytosis

Markus Mund, Aline Tschanz, Yu-Le Wu, Felix Frey, Johanna L. Mehl, Marko Kaksonen, Ori Avinoam, Ulrich S. Schwarz, Jonas Ries

Mund and Tschanz et al. reveal the three-dimensional shapes of clathrin coats during endocytosis, which partially preassemble and then bend progressively. They further introduce the novel Cooperative Curvature Model, which accurately describes the changes in shapes and dynamics during endocytic clathrin remodeling.

Article

| February 02 2023

Discrete activation pathways of the exocyst machine

Brittany K. Miller, Guendalina Rossi, Sara Hudson, David Cully, Richard W. Baker, Patrick Brennwald

Miller et al. identify the tomosyn homolog Sro7 as an allosteric activator of the exocyst resulting in specific biochemical changes to the complex. The outcomes of exocyst activation by Sro7 are distinct and insulated from exocyst activation by Rho/Cdc42 GTPases.

Article

| February 02 2023

Dynamin2 restrains caveola fission

Elin Larsson, Björn Morén, Kerrie-Ann McMahon, Robert G. Parton, Richard Lundmark

Larsson et al. demonstrate that the large GTPase dynamin 2 does not mediate fission of caveolae from the plasma membrane as previously thought. Instead, dynamin 2 associates with a subset of caveolae and functions to restrain caveolae to the plasma membrane via a GTPase-independent mechanism.

Article

| February 02 2023

Barbed end competition shapes actin networks

Alison C.E. Wirshing, Sofia Gonzalez Rodriguez, Bruce L. Goode

Competition at barbed ends between capping proteins and formins leads to the assembly of architecturally distinct cellular actin structures and the differential sorting of actin-binding proteins to each structure.

Article

| February 01 2023

Lack of p-Paxillin promotes cell migration in vivo

Qian Xue, Sophia R.S. Varady, Trinity Q Alaka’i Waddell, Mackenzie R. Roman, James Carrington, Minna Roh-Johnson

Xue et al. develop a system to visualize and compare focal adhesion formation and dynamics during single-cell migration in animal models versus cell culture systems. They reveal fundamental differences in the regulation of key focal adhesion proteins.

Report

| January 31 2023

PP4 ensures outer kinetochore assembly before NEBD

Helder Rocha, Patrícia A. Simões, Jacqueline Budrewicz, Pablo Lara-Gonzalez, Ana Xavier Carvalho, Julien Dumont, Arshad Desai, Reto Gassmann

Rocha et al. show that C. elegans protein phosphatase 4 ensures robust outer kinetochore assembly prior to nuclear envelope breakdown, which is critical for timely and proper engagement of mitotic chromosomes with spindle microtubules.

Article

| January 31 2023

α-tubulin protects cells from β-tubulin toxicity

Linnea C. Wethekam, Jeffrey K. Moore

Wethekam and Moore investigate how cells balance levels of α- and β-tubulin to supply αβ-heterodimers. They found that cells produce higher levels of α-tubulin than β-tubulin and require this excess to limit β-tubulin accumulation. β-tubulin accumulation causes microtubule loss and formation of dead-end tubulin assemblies.

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Volume 222,

Issue 2,

6 February 2023

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Reviews & Opinions

In Memoriam

| January 27 2023

Albert Reynolds (1956–2022): The father of p120

Gregg Wildenberg, Deborah J. Mariner, Panos Z. Anastasiadis, Micheal A. Davis, Renee Ireton, Huapeng Yu, Andrew L. Smith, Sarah Kurley, Juliet M. Daniel, Agnes Roczniak-Ferguson, Xiaobo Xia, Meredith Brown, Manish Tripathi, Molly Seale, Robert Carnahan, Whitney Smalley-Freed, Michael Dohn

This expansive work in Al’s career, however, was only part of the reason he earned the rightful title of “father of p120.” The other, often overlooked in science, was because of his generosity. We fondly remember occasions when we, as young graduate students prone to paranoia about being “scooped,” would be advised by Al ...

People & Ideas

| January 25 2023

Chii Jou Chan: The positives of being under "pressure"

Lucia Morgado-Palacin

Chii Jou Chan investigates how tissue mechanics and fluid pressure regulate mammalian development, with a special focus on folliculogenesis and oocyte quality control.

Spotlight

| January 25 2023

ASEM: An automated segmentation pipeline to detect a wide range of sub-cellular structures

Catherine G. Galbraith

Catherine Galbraith previews a Tools study from Gallusser and colleagues which describes a new pipeline for training a convolutional neural aimed at rapid automated detection of intracellular structures in volume electron microscopy images.

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