Autophagy selectively degrades a wide range of cellular components to regulate cellular functions or maintain cellular homeostasis. Tomioka et al. reveal that the nuclear pore complex and nucleoporins are degraded by selective autophagy upon inactivation of Tor kinase complex 1 in Saccharomyces cerevisiae.

Autophagy requires the synthesis of PI(3)P and the conjugation of LC3 to the phagophore membrane. We reconstituted these two reactions and their coupling by WIPI2 and showed that positive feedback between PI3KC3-C1 and WIPI2 leads to rapid LC3 lipidation by the ATG16L1 complex.

Sas4 is a conserved basal body assembly protein. Here, Ruehle et al. describe a previously unknown link between basal bodies and the control of cell division by Hippo signaling molecules that depends on Sas4.

To segregate chromosomes, the mammalian spindle must generate and respond to force. How it does so remains poorly understood. Pulling on the spindle using microneedles, Long et al. show that it can locally dissipate sustained force by regulating microtubule dynamics and breakage, thereby preserving global spindle structure.

TRPA1 is a nonselective cation channel implicated in thermosensation and inflammatory pain. Liu et al. validated that TRPA1 activation not only triggers Ca2+ influx through the plasma membrane, but also elicits Ca2+ release from endolysosomes, which directly triggers vesicle exocytosis and contributes to nocifensive sensation.

Hedgehog signaling involves the dynamic movement of receptors and effectors in and out of cilia. Desai et al. found that the dynamics of Smo are regulated by ubiquitination, which modulates its interaction with the intraflagellar transport system to control ciliary levels of this receptor.

Refutation of TRPA1-mediated lysosomal calcium release in sensory neurons, emphasizing the requirement and influx of extracellular calcium through TRPA1 for activation.