Shekhar and Hoeprich et al. show that Twinfilin bypasses the normal requirement for actin filament aging (Pi release) and drives barbed end depolymerization of newly formed filaments. This occurs even under assembly-promoting conditions, where actin monomer levels are far above the critical concentration.

A primordial function of the cGAS/STING pathway may be to induce LC3B lipidation. Fischer et al. show cGAMP-activated STING induces the V-ATPase to recruit ATG16L1 for lipidation of LC3B onto single-membrane perinuclear vesicles. The bacterial effector SopF blocks this pathway, suggesting roles in host defense.

Little is known about the functional importance of mRNA localization to centrosomes. Ryder et al. survey RNA localization to centrosomes within Drosophila embryos and show that regulation of centrocortin mRNA by FMRP ensures normal mitosis and embryonic viability.

Shinde et al. find that activation of G protein–coupled receptors (GPCRs) inside cilia triggers formation of ubiquitin chains onto the receptors via β-arrestin and that ubiquitin chains commit the GPCRs for exit from cilia.

Oxidative stress is a major risk factor for multiple disorders, including cataracts. The current study uncovers a unique mechanism that transports nutrients and antioxidants to maintain lens homeostasis and transparency. This process is fulfilled through a collaboration between integrin molecules and connexin channels.

Watanabe et al. show that centrioles and their associated pericentriolar matrix can independently support spindle assembly. They delineate how the pericentriolar matrix acts without centrioles and describe cancer cell lines in which spindles can assemble in the absence of both centrioles and pericentriolar matrix.

Stable eukaryotic flagella can maintain their length, and many protists grow new flagella while maintaining existing flagella. Mechanisms regulating this are not well understood. Atkins et al. show that CEP164C is important for this regulation, and these findings provide key novel insights into this process.