By systematic depletion of PCM proteins and centrioles in human cell lines, we reveal the cooperative role of the centriole and PCM scaffold proteins, pericentrin and CDK5RAP2, in the recruitment of CEP192 to spindle poles to promote bipolar spindle formation.

Kulasekaran et al. discover a novel GTPase cascade in which Arf5 activates Rab35. Disruption of this pathway increases EGF receptor activation, leading to upregulation of the transcription factor SPOCD1 with enhanced tumor growth and invasion in glioblastoma.

Murillo-Pineda et al. report a chromosome engineering system for human neocentromere formation and characterize the first experimentally generated human neocentromere. Neocentromere formation promotes local H3K9me3 eviction and cohesin and RNA polymerase II recruitment. Long-term culture results in gradual maturation of the inner centromere.

Orii et al. show that phospholipids distribute symmetrically in the yeast autophagosomal membranes. They also show that de novo–synthesized phosphatidylcholine is incorporated to autophagosomal membranes preferentially and attains the symmetrical distribution within 30 min after synthesis by an Atg9-dependent manner.

The small noncoding vault RNA is a component of the vault complex, a ribonucleoprotein complex found in most eukaryotes. Wakatsuki et al. show that vault RNA functions as a riboregulator of synaptogenesis by modulating the MAPK signaling pathway in neurons.

Watson et al. describe a general method to improve protein printing on glass (also known as micropatterning) while maintaining protein activity. In particular, this enables subcellular micropatterning, whereby complex micropatterns are able to simultaneously control cell shape and imprint the distribution of transmembrane receptors within that cell.

The intracellular position of lysosomes is critical for cell metabolism and signaling. Kesisova et al. discovered a membrane-associated septin GTPase scaffold of dynein–dynactin that promotes retrograde traffic and perinuclear lysosome clustering at steady state and in response to oxidative stress.