Membrane contact sites allow organelles to communicate with each other. Bisinski et al. show that Cvm1 is a novel component of three different contacts involving the yeast lysosome-like vacuole. These contacts both control and are regulated by the levels of sphingolipids in the cell.

Previous studies of chemotaxis have focused on protrusions and in vitro models. Georgantzoglou et al. show that protrusive structures in neutrophils only drive a first exploratory stage in vivo, while a second stage of actin flows and contractility is required for the ultimate motion response.

Michaud et al. identify Ect2 and RGA-3/4 as core components of the cortical excitability circuit associated with cytokinesis. Additionally, they demonstrate that the immature Xenopus oocyte is a powerful model for characterizing excitable dynamics.

Coon et al. identify an anti-inflammatory signaling pathway mediated by laminar shear stress stimulation of endothelial cells, whereby mitochondrial signaling and the adaptor protein, p62, promote ERK5 activation and enhance KLF2 expression. This accounts for relatively high KLF2 expression in laminar shear stimulated conditions compared to disturbed shear stimulation.

Zuidema et al. demonstrate that Tyr-635 phosphorylation of PEAK1 regulates cell migration through association with Tensin3 and localization of PEAK1 in focal adhesions.

Wang et al. report that the SARS-CoV-2 ORF10 promotes IFT46 degradation via stimulating CUL2^ZYG11B activity, thereby impairing both cilia biogenesis and maintenance. The study provides a pathological mechanism connecting COVID-19 symptoms with cilia dysfunction.

Fbxo7 has dual roles, ubiquitining proteins and activating the kinase Cdk6. The authors show both Fbxo7 functions converge on phosphofructokinase (PFKP), a gatekeeper of glycolysis, inhibiting its function. Fbxo7 deficiency increases glycolysis and reduces T cell activation and viability, demonstrating its important role in regulating metabolism.