Endocytosis is composed of early and late phases. This study demonstrates that the yeast Eps15/Pan1p is a key regulator for the late phase, and ectopic targeting of Pan1p to the peroxisome triggers most of the events occurring during the late phase at the peroxisome.

Park, Kim, Cho, et al. show that actin-regulatory Vav-Rac1-SCAR signaling independently regulates structural and functional presynaptic plasticity by driving BMP macropinocytosis and degradation of BMP receptors and mobilization of the reserve synaptic-vesicle pool, respectively. This work suggests that Vav differentially regulates distinct presynaptic actin pools with highly specialized tasks.

We reveal that the lipid droplets can emerge from ER subdomains in contact with mitochondria. The lipid transfer proteins ORP5 and ORP8 localize at these sites where they regulate the recruitment of seipin and ensure proper LD biogenesis.

Jiang et al. uncover that ubiquitylated RAP80 and BRCC36 are substrates of deubiquitylation within the BRCA1-A complex. Deubiquitylation prevents auto-inhibition of the complex, allowing RAP80 to bind ubiquitin at DNA damage sites. Autologous deubiquitylation explains the matching ubiquitin-binding and hydrolysis activities within the BRCA1-A complex.

Renne et al. show that Seipin promotes the formation of lipid droplets containing either triacylglycerol or steryl esters. It is proposed that interactions between hydroxyl-residues within the Seipin complex and neutral lipid carboxyl esters provide a general mechanism for Seipin-mediated LD formation.

Hannaford et al., identify that the interaction between Kinesin-1 and Pericentrin is essential for the direct transport of centrioles on the interphase microtubule network. This transport is important for the correct segregation of centrioles during asymmetric cell division.

Efficient and effective quantification protocol of membrane contact sites is an urgent demand in precise surveillance of various cellular processes. Liu et al. developed a deep learning–based high-throughput tool for profiling the contact with EM images.