On the cover
A two-photon image of the optic tectum of a living zebrafish larva shows a radial cluster of neurons expressing Lifeact-GFP. Lifeact expression is driven by pcdh19 regulatory elements, thus revealing that neurons expressing Protocadherin19 form neuronal columns.
Image © 2015 Cooper et al.
See page 807.
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Mammalian cells display a significant increase in volume during mitosis, which corresponds to a decrease in cell density, is linked to the mitotic state and not to the cell shape, and is independent of the actomyosin cortex.
Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway
Higher eukaryotes, but not yeast, are able to extract severely misfolded glycoproteins from the endoplasmic reticulum–associated degradation (ERAD) pathway for glycoproteins and target them to the ERAD pathway for non-glycoproteins to maintain the homeostasis of the ER.
Synaptonemal complex (SC) assembly requires polySUMOylation of Ecm11, which promotes polymerization of Zip1, the transverse filament, whereas the N terminus of Zip1 activates Ecm11 polySUMOylation, suggesting that this positive feedback loop underpins SC assembly.
Mice deficient for mitochondrial fission factor (Mff) die from severe cardiomyopathy, but cardiac function, tissue integrity, and mitochondrial respiration are robustly rescued by rebalancing mitochondrial dynamics through deletion of Mitofusin 1 (Mfn1).
δ-Protocadherins partition the zebrafish optic tectum into radial columns of neurons, and the neurons within a column are siblings derived from common neuronal progenitors.
Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression
In vertebrate embryos, the histone demethylase KDM4B affects otic placode proliferation, intercellular adhesion, and invagination by directly regulating Dlx3 expression.
Mutations in the seipin complex components Fld1 and Ldb16 result in the loss of lipid droplet identity and phospholipid packing defects, revealing a role of this complex in the stabilization of ER–lipid droplet contact sites.
The Numb/p53 circuitry couples replicative self-renewal and tumor suppression in mammary epithelial cells
A Numb/p53 circuitry exerts a potent tumor-suppressor function in the mammary gland by safeguarding against the emergence of cancer stem cells with unchecked self-renewal and tumorigenic potential.
PAK4, via a novel kinase-independent mechanism, protects RhoU from a Rab40A/Cullin 5 ubiquitin ligase complex–driven K48 ubiquitination to regulate breast cancer cell adhesion.
Oxidative stress induces EGFR-dependent phosphorylation and activation of ZNRF1 ubiquitin ligase in neurons, which promotes two major neurodegenerative pathways, apoptosis and axonal degeneration.
A protein complementation assay quantifying TDP-43 oligomerization in living neurons shows microvesicular and bidirectional synaptic transmission of TDP-43 and TDP-43 seeding activity in human ALS postmortem brain tissue.
Protein delivery based on bacterial type III secretion allows controllable injection of bacterial, viral, and human proteins into target cells, functional interaction studies, targeting of proteins to subcellular locations, and systems-level signaling analysis of a given translocated protein.