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Cover Image
Cover Image
On the cover
3D-STORM super-resolution imaging shows two neuronal axons approaching a dendritic shaft. Phalloidin staining of F-actin reveals the presence of both actin ring structures underlying the axonal membrane at regular intervals and deeper-lying actin filaments that extend in both directions along the axon.
Image © 2015 Ganguly et al.
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In This Issue
In Focus
Actin hotspots blaze a trail along axons
Study identifies a dynamic cytoskeletal network that delivers actin to presynaptic boutons.
People & Ideas
Jan van Deursen: From knockout pioneer to antiaging innovator
van Deursen’s career visits both sides of the cancer–senescence coin.
Feature
Report
TPX2 phosphorylation maintains metaphase spindle length by regulating microtubule flux
TPX2 is phosphorylated by Aurora A and is essential for normal microtubule flux on the metaphase spindle.
Article
Rapid pairing and resegregation of distant homologous loci enables double-strand break repair in bacteria
Double-strand break repair in Caulobacter is a dynamic process that can take place independent of DNA replication; resegregation of origin-proximal chromosomal regions after repair requires the ParABS system, whereas resegregation of origin-distal regions occurs independently of ParA and likely without dedicated segregation machinery.
A dynamic formin-dependent deep F-actin network in axons
Low-light live imaging of F-actin–selective probes, quantitative tools, and super-resolution microscopy reveals a dynamic, formin-dependent deep F-actin cytoskeletal network in axons.
Endocytosis-dependent coordination of multiple actin regulators is required for wound healing
Live imaging of epidermal wound closure in Drosophila embryos reveals that assembly of motile F-actin at wound edges requires the endocytic remodeling of adherens junctions and is mediated by Diaphanous, SCAR, and WASp.
MiT/TFE transcription factors are activated during mitophagy downstream of Parkin and Atg5
The transcription factor TFEB is activated in a Parkin- and Atg5-dependent manner during mitophagy, and MiT/TFE transcription factor family members are required for the efficient clearance of damaged mitochondria.
SNAREs support atlastin-mediated homotypic ER fusion in Saccharomyces cerevisiae
A quantitative in vitro assay based on isolated yeast microsomes reveals that SNARE-mediated membrane fusion is involved in atlastin-initiated homotypic ER fusion.
Lrrk regulates the dynamic profile of dendritic Golgi outposts through the golgin Lava lamp
Lrrk regulates Golgi outpost (GOP) dynamics in dendrites by antagonizing the interaction between the golgin Lva and dynein heavy chain at GOPs, thereby disrupting minus end–directed transport along dendritic microtubules by dynein.
PtdIns(4,5)P2 and PtdIns3P coordinate to regulate phagosomal sealing for apoptotic cell clearance
A coincidence detection mechanism regulates phagosomal sealing and couples it with phosphoinositide conversion from PtdIns(4,5)P2 enrichment on unsealed phagosomes to PtdIns3P enrichment on fully sealed phagosomes.
Adhesion to fibronectin regulates Hippo signaling via the FAK–Src–PI3K pathway
Fibronectin adhesion stimulation of focal adhesion kinase (FAK)–Src–PI3K is an upstream regulatory branch of the Hippo pathway and stimulates the activity and nuclear localization of YAP in a Lats-dependent manner.
