On the cover
Transmission electron microscopy reveals that budding yeast cells overexpressing Mdm1 form extensive contacts between their vacuoles and nuclear endoplasmic reticulum. Henne et al. report that mutations in Mdm1 disrupt nuclear ER–vacuole junctions and impair sphingolipid metabolism.
Image courtesy of Tony Carmello Gatts IV and © 2015 Henne et al.
See page 541.
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Paraspeckles are mammalian subnuclear bodies built on a long noncoding RNA and are enriched in RNA binding proteins with prion-like domains; two of these proteins, RBM14 and FUS, use these domains to hold paraspeckles together.
Mdm1 is a novel interorganelle tethering protein that localizes to yeast ER–vacuole/lysosome junctions, and Mdm1 truncations analogous to disease-associated Snx14 alleles fail to tether the ER and vacuole and perturb sphingolipid metabolism.
The geometry and arrangement of DNA loops in the pericentric region of the budding yeast centromere create a DNA-based molecular shock absorber that serves as the basis for how tension is generated between sister centromeres in mitosis.
TopBP1 maintains genome integrity in mitosis by controlling chromatin recruitment of SLX4 and by facilitating unscheduled DNA synthesis.
Non-muscle myosin IIB plays a major role in applying force on the nucleus to facilitate nuclear translocation through tight spaces during 3D invasive migration, while non-muscle myosin IIA is critical for generating force during active protrusion.
Photoreceptor outer segment (POS) phagosomes associate with the kinesin light chain 1 (KLC1) and move bidirectionally along microtubules in retinal pigment epithelium cells; lack of KLC1 results in impaired POS phagosome motility and degradation and, in aged mice, pathogenesis resembling age-related macular degeneration.
RELA and FOS are targets of miR-7 in gastric cancer cells and the miR-7/IKKε/RELA reciprocal feedback loop is important for gastric cancer induced by H. pylori infection.
Analysis of the cytosolic HIV-1 Gag fraction in live cells via advanced fluctuation imaging methods reveals potential nucleation steps before membrane-assisted Gag assembly.
The actin-binding domain of α-catenin directly interacts with actin in adherens junctions and may control the junction’s strength and dynamics by forming transient cadherin clusters bound to the actin cortex.
The shootin1–cortactin interaction participates in netrin-1–induced F-actin–adhesion coupling and in the promotion of traction forces for axon outgrowth.