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Cover Image
Cover Image
On the cover
Yan et al. reveal that the deubiquitinating enzyme complex BRISC regulates the localization and function of the spindle assembly factor NuMA. NuMA (green) forms a ring-like structure at the single spindle pole of a control cell treated with the Eg5 inhibitor STLC (bottom left), but is disorganized in cells lacking the BRISC subunits BRCC36 (top left) or ABRO1 (top right). Cells are also labeled for microtubules (red) and DNA (blue).
Image © 2015 Yan et al.
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In This Issue
In Focus
A timeline for exocytosis
Researchers track the tethering and fusion of individual secretory vesicles in budding yeast.
People & Ideas
Feng Shao: Getting a sense for the defense
Shao studies pathogenic bacteria and their hosts’ innate immune mechanisms.
From the Archive
A spotlight on chromatin choreography
In 1996, Robinett et al. developed a way to visualize chromatin structure and dynamics in living cells.
Review
Report
Tracking individual secretory vesicles during exocytosis reveals an ordered and regulated process
An analysis of individual secretory vesicles during yeast exocytosis explores how Sec4-GTP hydrolysis, Myo2p motors, an SM protein, and exocyst components contribute to the timing of vesicle tethering and fusion.
Article
BLM helicase facilitates telomere replication during leading strand synthesis of telomeres
BLM helicase facilitates telomere replication by resolving G-quadruplex structures that can form in the G-rich repeats during leading strand synthesis.
The deubiquitinating enzyme complex BRISC is required for proper mitotic spindle assembly in mammalian cells
The microtubule-associated protein BRISC regulates the interaction of NuMA with dynein and importin-β by removing K63-linked polyubiquitin chains from NuMA, thereby promoting proper bipolar spindle assembly.
ROCK1 and 2 differentially regulate actomyosin organization to drive cell and synaptic polarity
ROCK1 forms the stable actomyosin filament bundles that initiate front–back and dendritic spine polarity, while ROCK2 regulates contractile force, Rac1 activity, and cofilin-mediated actin remodeling at the leading edge of migratory cells and the spine head of neurons.
The small G-protein MglA connects to the MreB actin cytoskeleton at bacterial focal adhesions
The Ras-like small G-protein MglA is an integral part of the gliding motility complex at bacterial focal adhesions and stimulates the assembly of the motility complex by directly connecting it to the MreB actin cytoskeleton.
IQGAP1 promotes CXCR4 chemokine receptor function and trafficking via EEA-1+ endosomes
IQGAP1 mediates CXCR4 cell surface expression and signaling by regulating EEA-1+ endosome interactions with microtubules during CXCR4 trafficking and recycling.
Ypt1/Rab1 regulates Hrr25/CK1δ kinase activity in ER–Golgi traffic and macroautophagy
Ypt1 directly recruits the kinase Hrr25 to COPII vesicles to activate it in two different pathways: ER to Golgi and the catabolic macroautophagy pathway induced in response to cell stress.
Analysis of the interplay of protein biogenesis factors at the ribosome exit site reveals new role for NAC
The protein biogenesis factor NAC regulates the access of the enzyme MetAP and the signal recognition particle (SRP) to the ribosome, functions in SRP-dependent targeting, and can act to protect substrates from aggregation before translocation
Rme-8 depletion perturbs Notch recycling and predisposes to pathogenic signaling
The retromer-associated DNAJ protein Rme-8 is necessary for normal Notch recycling, and reductions in Rme-8 sensitize cells so that additional loss-of-sorting retromer or ESCRT-0 components have catastrophic effects.
CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface
The adaptor CIN85 enhances TGFβ-induced signaling and cellular responses to TGFβ by promoting the expression of TGFβ receptors on the surface in a Rab11-dependent manner.
The formation of ordered nanoclusters controls cadherin anchoring to actin and cell–cell contact fluidity
Visualization of single cadherins within cell membrane at nanometric resolution shows that E-cadherins arrange in ordered clusters and that these clusters control the anchoring of cadherin to actin and cell–cell contact fluidity.
Myosin 1b functions as an effector of EphB signaling to control cell repulsion
Myosin 1b functions as an effector of EphB2/ephrinB signaling and controls cell morphology and cell repulsion.
