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Cover Image
Cover Image
On the cover
Tmem231 (green) localizes to the transition zone between the basal body (red) and axoneme of primary cilia (blue). The nuclei of these mouse embryonic fibroblasts are labeled magenta. Roberson et al. reveal that Tmem231, whose gene is mutated in both Meckel syndrome and orofaciodigital syndrome type 3, organizes the transition zone in order to control ciliary membrane composition.
Image © 2015 Roberson et al.
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In This Issue
In Focus
PI(4)P gets Sac-rificed in the name of endocytic recycling
Two studies reveal that Sac2 acts as a phosphoinositide 4-phosphatase on early endosomes.
People & Ideas
Dyche Mullins: Finding filaments at the fringes
Mullins has made his mark by exploring the actin cytoskeleton’s frontiers.
Feature
Review
Report
A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front–back polarity
A group of phosphorylatable serine residues within the nonhelical domain of NMII-B controls the ability of NMII-B to generate stable migratory front–rear polarity.
Article
Excision of translesion synthesis errors orchestrates responses to helix-distorting DNA lesions
An Msh2/Msh6-dependent DNA repair mechanism mitigates the mutagenicity of photolesions and induces cell cycle responses by excising incorrect nucleotides incorporated by postreplicative translesion synthesis.
Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
Long-term imaging via microfluidic chambers shows that two minus end–directed motors, dynein and Klp2, work in parallel at distinct subcellular structures to promote efficient nuclear congression.
A role for disulfide bonding in keratin intermediate filament organization and dynamics in skin keratinocytes
Disulfide bonds involving cysteine 367 in K14 play a crucial role in the assembly, dynamics, and organization of K14-containing filaments in epidermal keratinocytes.
G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
The TDP-43 target G3BP1 is essential for a functional interaction between stress granules and processing bodies.
Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway
The function of Sac2/INPP5F in the endocytic pathway and its activity as a 4-phosphatase suggest that Sac2/INPP5F and OCRL may cooperate in the sequential dephosphorylation of PI(4,5)P2 in a partnership that mimics that of the two phosphatase modules of synaptojanin.
Spatiotemporal control of phosphatidylinositol 4-phosphate by Sac2 regulates endocytic recycling
Sac2 (INPP5F) is a phosphoinositide 4-phosphatase that specifically hydrolyzes PI(4)P and regulates endocytic recycling.
Phosphorylated ubiquitin chain is the genuine Parkin receptor
PINK1-phosphorylated ubiquitin chain is the genuine Parkin receptor that recruits Parkin to depolarized mitochondria.
TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone
TMEM231, a functional component of the MKS complex at the ciliary transition zone, is mutated in orofaciodigital syndrome type 3 and Meckel syndrome.
TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse
TAGLN2 stabilizes cortical F-actin and thereby maintains F-actin contents at the immunological synapse, which allows T cell activation following T cell receptor stimulation.
Tools
Open source software for quantification of cell migration, protrusions, and fluorescence intensities
ADAPT is an ImageJ plug-in that can be used for rapid whole-cell analysis of time-lapse videos, thereby providing data on cell morphology, membrane velocity, and temporal changes in any fluorescent protein of interest at the cell periphery, as exemplified by the morphological characterization of cellular blebs.
