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Cover Image
Cover Image
On the cover
Structured illumination microscopy shows the distribution of actin (green), myosin light chain phosphorylated on serine 19 (red), and phosphotyrosine-positive focal adhesions (blue) in keratocytes isolated from zebrafish embryos at two (top left) and four (bottom right) days postfertilization. Lou et al. reveal that increased expression of myosin light chain kinase at four days postfertilization promotes the formation of multiple protrusions instead of the single, broad lamellipodium formed by keratocytes isolated from younger embryos.
Image © 2015 Lou et al.
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In This Issue
In Focus
Sugar saps FOXO1’s healing power
Study finds that high glucose levels cause protein to thwart cell migration.
People & Ideas
Matthew Rasband: Zen and the art of axonal maintenance
Rasband studies clustering of axonal voltage-gated sodium channels.
From the Archive
Demystifying Aurora B
In 2003, two papers uncovered the mitotic functions of Aurora B using novel small molecule inhibitors.
Editorial
Review
Report
Glutamylation of Nap1 modulates histone H1 dynamics and chromosome condensation in Xenopus
Nap1 is required for linker histone H1M-mediated mitotic chromosome condensation in Xenopus egg extracts, and glutamylation of Nap1 is required for proper deposition and turnover of H1M on chromatin during both interphase and mitosis.
TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment
The TRAPP subunit TrappC12/TTC15, here renamed TRAMM, plays a role in the regulation of kinetochore stability and CENP-E recruitment during mitosis.
Splicing function of mitotic regulators links R-loop–mediated DNA damage to tumor cell killing
Mitotic regulators BuGZ and Bub3 play a critical role in RNA splicing during interphase, and disruption of this function leads to R-loop formation, DNA damage, and p53 activation.
Article
Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis
A refined spindle pole body (SPB) affinity purification method reveals that the telomere-associated protein Ndj1 also localizes to yeast SPBs, protects them from premature separation, and therefore regulates both SPB cohesion and telomere clustering during meiosis.
The yeast ERAD-C ubiquitin ligase Doa10 recognizes an intramembrane degron
In Saccharomyces cerevisiae, surprisingly, the transmembrane protein Sbh2, which harbors an intramembrane degron, is a substrate of the ubiquitin-protein ligase Doa10.
Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase
Use of embryonic zebrafish keratocytes as a model system shows that increased myosin light chain kinase (MLCK) activity promotes the formation of multiple protrusions independently of ROCK by increasing myosin accumulation in lamellipodia.
FOXO1 differentially regulates both normal and diabetic wound healing
FOXO1 differentially regulates normal and diabetic wound healing through a switch in downstream targets influenced by the effect of glucose and advanced glycation end products.
Cell motion predicts human epidermal stemness
Keratinocyte stem cell colonies can be identified by analyzing cell motion, an emergent stem cell property.
