On the cover
Wolf et al. explore the physical limits of cell migration. A cell labeled for F-actin (cyan) tries to move its nucleus (red/yellow) through a high-density lattice of rat tail collagen (grey) in the absence of matrix metalloproteinase activity.
Image © 2013 Wolf et al.
See page 1069.
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In This Issue
Testing the limits of cell migration
Migrating cells are restricted by their ability to squeeze their nuclei through pores in the extracellular matrix.
People & Ideas
John Briggs: A closer look at HIV and coated vesicles
Briggs studies the organization of viral and cellular coat proteins using cryo-electron microscopy.
Replication and trafficking of a plant virus are coupled at the entrances of plasmodesmata
Plant virus movement proteins compartmentalize replication complexes at plasmodesmata for localized RNA synthesis and directional trafficking of the virus between cells.
The tumor suppressor CDKN3 controls mitosis
A genome-wide screen of phosphatases that control mitosis identified CDKN3, which acts through the CDC2 signaling axis.
The spindle checkpoint, APC/CCdc20, and APC/CCdh1 play distinct roles in connecting mitosis to S phase
The spindle checkpoint, APC/C-Cdc20, and APC/C-Cdh1 act successively to connect disappearance of geminin and cyclin B1 to a peak of Cdt1 and Cdc6.
The dynamics of engineered resident proteins in the mammalian Golgi complex relies on cisternal maturation
Engineered Golgi-resident constructs that can be polymerized at will to prevent their recycling via Golgi carriers provide evidence for the cisternal maturation mechanism of secretory protein transport through the Golgi.
Cyclin-dependent kinases regulate splice-specific targeting of dynamin-related protein 1 to microtubules
The splice isoform Drp1-x01 promotes mitochondrial fission and is regulated by Cdk phosphorylation-dependent changes in microtubule association.
Complete integrin headpiece opening in eight steps
Crystals soaked with RGD peptides reveal six intermediate conformational states between the closed and higher affinity, fully open state of the integrin αIIbβ3 headpiece.
Physical limits of cell migration: Control by ECM space and nuclear deformation and tuning by proteolysis and traction force
The physical limits of cell migration in dense porous environments are dependent upon the available space and the deformability of the nucleus and are modulated by matrix metalloproteinases, integrins and actomyosin function.