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Cover Image
Cover Image
On the cover
Lewellyn et al. describe how the protein kinase Misshapen decreases integrin levels to promote the migration of follicle cells during Drosophila egg chamber morphogenesis. In an egg chamber stained for cortical actin (red), wild-type follicle cells (marked with GFP, green) break away from a group of immotile, Misshapen-null cells and bisect the oocyte by invading the inner germ cell cluster.
Image © 2013 Lewellyn et al.
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In This Issue
In Focus
The plastic proteome
Researchers catalog how stresses affect the abundance and localization of every budding yeast protein.
People & Ideas
Jürgen Roth: Immunogold master
Roth pioneered immunogold EM on thin sections and studies protein glycosylation.
Review
Report
Oncogenes induce genotoxic stress by mitotic processing of unusual replication intermediates
Processing of unusual replication intermediates such as reversed forks by MUS81 contributes to oncogene-induced double-strand breaks and depends on mitotic entry.
Rer1p maintains ciliary length and signaling by regulating γ-secretase activity and Foxj1a levels
Rer1p is an ER/cis-Golgi membrane protein that maintains ciliary length and function by reducing γ-secretase complex assembly and activity (thereby balancing Notch signaling) and increasing Foxj1a expression.
Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila
Misshapen promotes individual cell motility in the Drosophila follicular epithelium through a mechanism that appears to reduce integrin levels at each cell’s trailing edge.
Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration
Cortical layer 5 pyramidal neurons and spinal cord motor neurons are selectively vulnerable to degeneration after loss of the autophagy gene Epg5.
Article
CUL4B promotes replication licensing by up-regulating the CDK2–CDC6 cascade
CUL4B up-regulates CDK2 by repressing miR-372 and miR-373, leading to increased phosphorylation and stabilization of CDC6, thus promoting replication licensing.
Pericentric chromatin loops function as a nonlinear spring in mitotic force balance
During mitosis, cohesin- and condensin-based pericentric chromatin loops function as a spring network to balance spindle microtubule force.
MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression
MISP is a substrate of Plk1 and important for the stabilization of cortical and astral microtubule attachments to regulate mitotic spindle positioning.
Bidirectional Ca2+ signaling occurs between the endoplasmic reticulum and acidic organelles
After acidic organelles induce signaling to activate ER calcium ion release, local microdomains of high calcium at ER–acidic organelle junctions feed back to activate further acidic organelle calcium release.
The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit
Sicily, which was identified in a screen for proteins involved in neurodegeneration, interacts with cytosolic Hsp90 to chaperone the complex I subunit ND42, before its mitochondrial import.
Vps35 loss promotes hyperresorptive osteoclastogenesis and osteoporosis via sustained RANKL signaling
Vps35 deficiency leads to impaired RANK trafficking, enhanced RANKL signaling, increased osteoclastogenesis and function, and osteoporotic deficits.
Tools
A novel single-cell screening platform reveals proteome plasticity during yeast stress responses
Unprecedented proteome plasticity in response to stress in yeast is revealed using a novel screening platform that allows tracking of protein localization and abundance at single-cell resolution.
