Oncogene-induced DNA replication stress activates the DNA damage response (DDR), a crucial anticancer barrier. DDR inactivation in these conditions promotes genome instability and tumor progression, but the underlying molecular mechanisms are elusive. We found that overexpression of both Cyclin E and Cdc25A rapidly slowed down replication forks and induced fork reversal, suggestive of increased topological stress. Surprisingly, these phenotypes, per se, are neither associated with chromosomal breakage nor with significant DDR activation. Oncogene-induced DNA breakage and DDR activation instead occurred upon persistent G2/M arrest or, in a checkpoint-defective context, upon premature CDK1 activation. Depletion of MUS81, a cell cycle–regulated nuclease, markedly limited chromosomal breakage and led to further accumulation of reversed forks. We propose that nucleolytic processing of unusual replication intermediates mediates oncogene-induced genotoxicity and that limiting such processing to mitosis is a central anti-tumorigenic function of the DNA damage checkpoints.
Activation of a growing number of oncogenes has been found associated with “replication stress,” a poorly understood perturbation of DNA replication (Mailand et al., 2000; Bartkova et al., 2005, 2006; Gorgoulis et al., 2005; Di Micco et al., 2006; Dominguez-Sola et al., 2007). Replication stress induces the activation of the DNA damage response (DDR), which is detected from the earliest stages of tumorigenesis (Bartek et al., 2007). Oncogene-induced replication stress is associated with the formation of double-strand breaks (DSBs), particularly in regions intrinsically difficult to replicate (Bartkova et al., 2005, 2006; Gorgoulis et al., 2005). The observed DDR activation induces senescence in precancerous lesions and functions as a barrier against full malignant transformation (Bartkova et al., 2006; Di Micco et al., 2006).
Oncogene activation affects—directly or via deregulation of CDK2—the replication initiation program, resulting in deregulated origin firing and impaired fork progression. The latter effect is proposed to result from nucleotide depletion (Bester et al., 2011), from interference between DNA replication and transcription (Jones et al., 2012), and/or from increased DNA torsional stress (Bermejo et al., 2012), but the lack of structural information on replication intermediates (RIs) under these experimental conditions has so far limited our understanding of the underlying mechanisms. Furthermore, it is unclear how perturbations at the replication forks lead to DSB formation that promotes chromosomal rearrangements during tumorigenesis.
Replication stress has been recently associated with transient accumulation of DNA lesions and large 53BP1 foci formed when cells progress through mitosis (Lukas et al., 2011). It was previously reported that different oncogenes lead to mitotic aberrations (Molinari et al., 2000; Ichijima et al., 2010), but the causative link between these phenotypes and oncogene-induced genotoxic stress has remained obscure. Recently, additional molecular events of potential importance for chromosomal integrity were associated with mitotic entry, e.g., the resolution of Holliday junctions (HJs), central intermediates of DNA homologous recombination (HR; Matos et al., 2011; Schwartz and Heyer, 2011). Furthermore, the HJ resolvase MUS81 was recently implicated in DSB formation upon oncogene overexpression (OE) or cell cycle perturbation, but the link to mitotic progression is controversial and the underlying mechanisms remained elusive (Beck et al., 2010; Domínguez-Kelly et al., 2011; Forment et al., 2011; Murfuni et al., 2013).
In this work, comparing OE of Cyclin E (CycE) and Cdc25A, we identify reversed forks as common unusual replication intermediates, suggesting increased topological stress as a critical determinant of oncogene-induced replication stress. Surprisingly, fork slowing and restructuring are, per se, neither associated with chromosomal breakage nor with full DDR activation. We show that processing of these unusual replication intermediates by MUS81 depends on mitotic entry and contributes to oncogene-induced DSBs. Premature CDK1 activation upon checkpoint inactivation accelerates and exacerbates oncogene-induced DSB formation, providing a mechanistic rationale for the function of the DNA damage checkpoints as barriers against genome instability. Thus, specific DNA structures and cell cycle transitions mediate oncogene-induced chromosomal breakage.
Results and discussion
Oncogene OE rapidly interferes with replication fork progression and induces fork reversal
To elucidate the impact of oncogenes on the replication process, we focused on two established systems of oncogene OE (Mailand et al., 2000; Bartkova et al., 2005) as prototypes of two different scenarios: (1) “Oncogene OE only” by CycE OE, where CDK2 hyperactivation deregulates DNA replication; and (2) “Oncogene OE + checkpoint defect” by Cdc25A OE. As this phosphatase is at the same time a CDK activator and a key effector of the DNA damage response (Mailand et al., 2000), Cdc25A OE combines CDK2 deregulation with impaired cell cycle control, two key steps in tumorigenesis (Bartkova et al., 2006; Di Micco et al., 2006).
We tested the effect of both oncogenes on the progression of individual replication forks by DNA fiber spreading analysis (Fig. 1 A; Jackson and Pombo, 1998). In keeping with two recent reports (Bester et al., 2011; Jones et al., 2012), oncogene OE is associated with a significant replication fork slowdown. We now show that this fork delay does not require prolonged oncogene expression (Bester et al., 2011), but is detectable in both systems from the earliest time point (8 h; Fig. 1, A–C). Next, we investigated in vivo replication fork architecture upon oncogene OE by electron microscopy (EM; Neelsen et al., 2013). Our most striking observation was the accumulation of reversed forks (RFs), i.e., replication forks showing a fourth regressed arm, due to local annealing of the newly synthesized strands (Fig. 1, D and E). Although these are rare intermediates during unperturbed S phase, they rapidly accumulate upon OE of both CycE and Cdc25A (Fig. 1, D–G; and Fig. S1 A). Furthermore, their frequency does not further increase at later time points (Fig. 1, F and G). Upon OE of both oncogenes, we also detected an increasing number of molecules exposing extended single-stranded DNA (ssDNA) regions, mainly as gaps on newly synthesized strands, but also at the replication fork (Fig. S1, B–F). In Cdc25A-overexpressing cells, a few replication bubbles showed one side entirely single stranded (hemireplicated bubble; Fig. S1 C; Sogo et al., 2002). Moreover, in a subset of RIs from later time points of CycE OE, we occasionally detected ssDNA gaps on the parental strands (Fig. S1 D), suggesting that ssDNA regions are carried over from the previous round of replication. To exclude that our results are specific for cancer cells, we transiently overexpressed both oncogenes in untransformed human MRC5 fibroblasts. Transient CycE and Cdc25A OE in MRC5 resulted in replication fork slowdown similar to that in U2OS cells, and was accompanied by the accumulation of RF- and ssDNA-containing molecules (Fig. S2, A–D). Overall, these data identify common features of DNA replication stress—i.e., fork slowing, fork reversal, and ssDNA accumulation—detectable with similar kinetics upon OE of both oncogenes, and provide direct evidence that oncogene OE affects the structure of replication intermediates in vivo.
Cell cycle progression, chromosomal breakage, and DDR upon CycE and Cdc25A OE
We next tested whether replication stress is associated with DSBs, cell cycle arrest, and DDR activation. CycE OE leads to transient accumulation of cells in S and G2/M phases, followed at late time points (4–8 d) by accumulation of rereplicating cells (DNA content >4n; Fig. 2 A). Cell cycle deregulation by CycE OE delayed proliferation only three days after oncogene induction (Fig. 2 B). Mild ATR/CHK1 activation was detectable within 24 h of CycE OE, whereas DNA breakage—as assessed by pulse-field gel electrophoresis (PFGE) —and ATM/KAP1 phosphorylation were detected only after 4–8 d (Fig. 2, C and D), when the cells accumulate in G2/M and occasionally rereplicate (Fig. 2 A). Thus, in this experimental system, oncogene-induced fork slowing and reversal precede and are not directly associated with DSBs (Fig. 1, F and G; and Fig. 2). In contrast, Cdc25A OE leads to a marked arrest of cell cycle and proliferation already one day after induction (Fig. 2, E and F). ATR/CHK1 activation was detectable early after oncogene OE (8 h) and was rapidly associated with massive DNA breakage (16–24 h) and ATM/KAP1 activation (Fig. 2, G and H).
Transient OE of CycE and Cdc25A in MRC5 yielded similar results, in that CycE OE for 48 h neither caused DSB nor DDR activation, whereas Cdc25A OE resulted in DSB formation and stepwise activation of ATR/CHK1 and ATM/KAP1 (Fig. S2, E and F). Overall, CDK2 deregulation by both CycE and Cdc25A OE rapidly induces prolonged S phase and ATR activation, and accumulation of unusual replication intermediates. However, chromosomal breakage and cell cycle arrest occur with strikingly different kinetics in the two systems.
Marked replication stress does not activate the DDR until cells experience a persistent G2/M arrest (CycE) or a premature replication block (Cdc25A)
To further characterize oncogene-induced DNA breakage and DDR, we studied H2AX phosphorylation (γ-H2AX) and 53BP1 recruitment by single-cell immunofluorescence (IF). Whereas the former event marks sites of DNA damage in general, the latter is more specific for DSBs (de Feraudy et al., 2010; Ray Chaudhuri et al., 2012). Despite the marked replication phenotypes (Figs. 1 and S2), CycE-overexpressing cells showed no γ-H2AX above background for 2–3 d after induction. 10–20% of the cells did show γ-H2AX foci at later time points (4–8 d), when DSBs become physically detectable (Figs. 2 C, 3 A, and S3 A). Coupling IF-based γ-H2AX detection with flow-cytometric analysis of DNA content (DAPI) and replication (5-ethynyl-2′-deoxyuridine [EdU]; Fig. S3, B and C), we observed that increased γ-H2AX levels upon prolonged CycE OE are mainly present in cells accumulating in G2/M or attempting rereplication (Fig. 3 B). Rereplicating cells were also easily identified by IF microscopy because of their “giant nuclei” (Zhu et al., 2004) and displayed accumulation of γ-H2AX foci, mostly colocalizing with 53BP1, thus marking DSBs (Fig. S3 D). In contrast, the DDR observed upon Cdc25A OE was more rapid and heterogeneous. Cells with γ-H2AX/53BP1 foci were detected 8 h after Cdc25A induction, whereas after 24 h a significant fraction of cells showed intense pan-nuclear γ-H2AX (Fig. 3, C and D), a phenotype previously associated with replication stress (Murga et al., 2009). Pan-nuclear γ-H2AX was consistently associated with intermediate DNA content and compromised EdU incorporation, suggesting a replicative arrest (Fig. 3 E). These cells also consistently show intense DAPI staining and, in spite of the high γ-H2AX signal, are devoid of 53BP1 foci (Fig. 3 C). Both features are characteristic of mitotic chromatin condensation (Giunta et al., 2010). Cdc25A OE also rapidly induced an increase in nuclear fragmentation, frequently associated with pan-nuclear γ-H2AX (Fig. 3 C; Fig. S3, E and F). A similar phenotype was associated with prolonged CycE OE (Fig. S2 G). In summary, CycE-overexpressing cells show mild and slow accumulation of DSB markers in IF and FACS. In contrast, these markers are rapidly detectable upon Cdc25A OE and correlate with mitotic features in cells experiencing a replicative arrest.
Massive chromosomal breakage and DDR are associated with premature activation of mitotic markers and suppressed by CDK1 inhibition
The results shown so far imply that oncogene-induced replication stress does not directly lead to DSB formation. However, they suggest that oncogene-induced DSBs occur either in mitosis or upon premature mitotic entry. To test this hypothesis, we used a broad-spectrum marker of mitotic CDK1-activity—the MPM-2 antibody, which recognizes an abundant phospho-epitope on CDK1 substrates (Davis et al., 1983)—and found that 24 h after Cdc25A induction this marker is no longer restricted to mitotic cells, but detectable also in a substantial fraction of cells with intermediate DNA content (Fig. 4, A and B). Furthermore, intense γ-H2AX in Cdc25A-overexpressing cells is associated with elevated levels of MPM-2 (Fig. 4 A), establishing a link between premature mitotic entry and oncogene-induced DDR activation. To assess directly whether initiation of mitosis is required for Cdc25A-induced DNA breakage, we overexpressed the oncogene in the presence of the CDK1 inhibitor RO-3306 (Vassilev et al., 2006). CDK1 inhibition did not interfere with oncogene expression, allowed transit into S phase, and did not affect the initial (8 h) increase of CHK1 activity induced by Cdc25A OE (Figs. 4 C and S3 I). However, Cdc25A-induced DSB and ATM activation were completely suppressed (Fig. 4, C–E). Accordingly, CHK1 phosphorylation after 12–16 h, presumably resulting from DSB resection, was also suppressed (Fig. 4 C). In checkpoint-proficient cells, the ATR pathway restricts CDK1 activity in response to replication stress. To test whether ATR limits oncogene-induced DNA breakage, we inhibited ATR in CycE-overexpressing cells (Toledo et al., 2011). Indeed, ATR inhibition increased the amount of CycE-induced DSBs after prolonged CycE OE (Fig. S3, J–L). Taken together, our data indicate that the massive chromosomal breakage induced by Cdc25A OE is associated with and depends on premature initiation of mitosis.
Reversed forks are MUS81 substrates and precursors of oncogene-induced DSBs
The HJ resolvase MUS81-EME1 was previously suggested to process replication forks after prolonged arrest (Hanada et al., 2006, 2007), and was also recently implicated in oncogene-induced genotoxicity (Murfuni et al., 2013), but neither its mechanistic role nor its substrates have been identified. We found that siRNA-mediated MUS81 depletion suppressed up to 60% of Cdc25A-induced breaks (Fig. 4, F–H; and Fig. S3 M). As we identified RFs as an abundant unusual intermediate upon oncogene OE, we tested the hypothesis that they are substrates for cleavage by MUS81. Indeed, MUS81 depletion caused an increase in Cdc25A-induced RFs, strictly correlating with the amount of residual protein and the decrease of DSBs (Fig. 4, F–I). A reproducible increase in RF frequency was also observed in the absence of oncogene OE, suggesting that occasional RFs formed in unperturbed conditions are targeted by MUS81. Overall these data show that RFs are substrates for MUS81 cleavage and precursors of oncogene-induced DNA breakage.
Our work sheds light on several important mechanistic aspects of oncogene-induced genotoxicity. We present the first direct visualization of the impact of oncogene OE on the structure of RIs in vivo. Although the observed accumulation of ssDNA was predicted by previous studies (Bartkova et al., 2005), it is surprising that replication forks challenged by OE of both CycE and Cdc25A regress rapidly and frequently. Reversed forks were first shown in bacteria in response to torsional stress (Postow et al., 2001). They had been long postulated also in eukaryotic cells (Higgins et al., 1976) and were shown to arise in yeast upon topological impediments induced by checkpoint defects (Sogo et al., 2002; Bermejo et al., 2011). Most recently, reversed forks have been reported as frequent RI upon topoisomerase I poisoning also in higher eukaryotes (Ray Chaudhuri et al., 2012). Overall, the available data establish fork reversal as an evolutionary conserved response to topological constraints. Our structural observations support a scenario where oncogene OE impairs the replication process by inducing topological stress resulting from deregulating simultaneously CDK2-dependent origin firing and transcription (Fig. 5; Bermejo et al., 2012). In line with this notion, most oncogenes shown to induce replication stress deregulate the G1–S transition (Bartkova et al., 2005, 2006; Di Micco et al., 2006). Accordingly, CycE-induced fork slowing was recently linked to high levels of replication initiation and the resulting interference between replication and transcription (Jones et al., 2012). Prolonged exposure to oncogenic stress was also recently linked to nucleotide depletion (Bester et al., 2011), which may arise as a consequence of supernumerary replication factories and contribute to replication fork stalling.
A second unexpected conclusion of our work is that the manifestations of oncogene-induced replication stress (fork reversal and ssDNA exposure) are not, per se, leading to chromosomal breakage, but are tolerated without potent DDR activation for at least three cell cycles upon CycE OE. In contrast, Cdc25A-overexpressing cells show massive DNA breakage, pan-nuclear γ-H2AX, and cell cycle arrest already 16–24 h after induction. Our data identify the critical determinant of this difference in the regulation of CDK1-dependent mitotic entry, which is maintained upon oncogene OE (CycE OE), but lost with severe cellular consequences upon inactivation of cell cycle checkpoints (Cdc25A OE), often associated with malignant transformation. We propose that the replication stress observed early after oncogene induction is tolerated in CycE-overexpressing cells by means of controlled processing of replication intermediates during a transient, checkpoint-mediated delay in G2/M (Lukas et al., 2011). This transient arrest limits DNA breakage, as ATR inhibition under these conditions leads to an increase in CycE-induced DSBs (Fig. S3, J–L). However, the accumulation of “DNA lesions” upon prolonged CycE OE (Figs. 1 and S1) may eventually lead to a persistent G2/M arrest, extensive processing of replication intermediates, inaccurate chromosome segregation (e.g., micronuclei; Fig. S3 G), and abortive attempts to restart DNA replication (Fig. 5 A; Di Micco et al., 2006; Davoli et al., 2010; Crasta et al., 2012). In contrast, Cdc25A-overexpressing cells—prototypic of checkpoint-deficient cells experiencing oncogenic stress—display constitutive CDK1 hyperactivation and are therefore unable to restrain mitotic processing, which is initiated together with the earliest manifestations of replication stress. Thus, they rapidly incur extensive DNA breakage, strong DDR activation, and replicative arrest. We propose that the crucial role of the DDR as an anticancer barrier results from its molecular function in the tolerance of oncogene-induced replication stress, by ensuring controlled processing of unusual RI and thus preventing excessive chromosomal breakage.
Finally, our work provides important mechanistic insight in the cellular activities involved in oncogene-induced chromosomal breakage. Combining PFGE and EM analysis, we show that MUS81/EME1—a cell cycle–regulated HJ resolvase (Matos et al., 2011)—is a major contributor to Cdc25A-induced DSBs by processing reversed forks (Fig. 5 B). These data demonstrate that HJs at replication forks are in vivo substrates of this nuclease, despite the weak cleavage activity in vitro (Taylor and McGowan, 2008). Interestingly, at least a fraction of reversed forks displays ss-nicks or gaps at the branch point (Figs. 4 J and S1 A), which are known to enhance susceptibility to cleavage by MUS81 (Schwartz and Heyer, 2011). Furthermore, the observation that MUS81 controls the abundance of reversed forks also in unperturbed conditions suggests that these structures are formed even in the absence of exogenous stress and that their controlled processing is required for genome maintenance in every cell cycle.
Materials and methods
Cell culture, treatments, and transfections
U2OS-derived clones carrying inducible copies of CycE and Cdc25A were grown in DMEM + 10% FCS supplemented with 4 µg/ml tetracycline. Oncogene expression was induced by washing off tetracycline. MRC5 cells were grown in DMEM + 10% FCS. For inhibition of CDK1, RO-3306 (#217699; EMD Millipore) was added 3 h after oncogene induction at a final concentration of 9 µM. Tetracycline was from Sigma-Aldrich (T7660). For inhibition of ATR, the ATR inhibitor ETP-46464 (kindly provided by O. Fernandez-Capetillo, CNIO, Madrid, Spain) was added for 12 h before collection at a final concentration of 2 µM. For oncogene OE in MRC5 cells, cells were transfected with pBabe (empty vector), or plasmid encoding Cdc25A or CycE (kindly provided by J. Lukas, Center for Protein Research, Copenhagen, Denmark), respectively, at the indicated time points before collection using FuGENE6 (Promega) according to the manufacturer’s instructions. For depletion experiments, cells were transfected 72 h before oncogene induction with the indicated siRNA using RNAiMax (Invitrogen) according to the manufacturer’s instructions. siLUC (33 nM; 5′-GGUACGCGGAAUACUUCGAdTdT-3′), siMUS81#1 (33 nM; 5′-CAGCCCUGGUGGAUCGAUAdTdT-3′), and siMUS81#2 (80 nM; 5′-CAGGAGCCAUCAAGAAUAAdTdT-3′).
Cell cycle analysis by propidium iodide staining was performed as described previously (Toller et al., 2011). In brief, cells were fixed with ice-cold 70% ethanol, washed with PBS, pH 7.4, and DNA was stained with 25 µg/ml propidium iodide (Sigma-Aldrich). For flow cytometric analysis for γ-H2AX/EdU/DAPI, cells were labeled for 30 min with 10 µM EdU, harvested, and fixed for 10 min with 4% formaldehyde/PBS. Cells were washed with 1% BSA/PBS, pH 7.4, permeabilized with 0.5% saponin/1% BSA/PBS, and stained with anti–γ-H2AX antibody (#05-636; EMD Millipore) for 2 h, followed by incubation with a suitable secondary antibody for 30 min. Incorporated EdU was labeled according to the manufacturer’s instructions (#C35002; Invitrogen). For flow cytometric analysis for γ-H2AX/MPM-2/DAPI, cells were fixed and permeabilized as described above, followed by incubation with antibodies against γ-H2AX (#9718; Cell Signaling Technology) and MPM-2 (#05-368; EMD Millipore) and suitable secondary antibodies. In both assays, DNA was stained with 1 µg/ml DAPI. Samples were measured on a Cyan ADP flow cytometer (Beckman Coulter) and analyzed with Summit software v4.3 (Beckman Coulter).
Pulse-field gel electrophoresis, single-cell microscopy, and antibodies
Pulse-field gel electrophoresis was performed as reported previously (Toller et al., 2011). In brief, cells were embedded in a 0.8% agarose plug (2.5 × 105 cells/plug), digested in lysis buffer (100 mM EDTA, 1% [wt/vol] sodium lauryl sarcosyne, 0.2% [wt/vol] sodium deoxycholate, and 1 mg/ml proteinase K) at 37°C for 48 h, and washed in 10 mM Tris-HCl, pH 8.0, and 100 mM EDTA. Electrophoresis was performed at 14°C in 0.9% (wt/vol) Pulsed Field Certified Agarose (Bio-Rad Laboratories) containing Tris-borate/EDTA buffer in a CHEF DR III apparatus (9 h, 120°, 5.5 V/cm, 30–18 s switch time; 6 h, 117°, 4.5 V/cm, 18–9 s switch time; 6 h, 112°, 4 V/cm, 9–5 s switch time; Bio-Rad Laboratories). The gel was stained with ethidium bromide and imaged on an Alpha Innotech Imager. For single-cell immunostaining, cells were fixed with 4% formaldehyde/PBS, stained for γ-H2AX and 53BP1 as indicated, detected by appropriate secondary antibodies, and mounted with Vectashield (Vector Laboratories). Cells were imaged using a microscope (model DMRB; Leica) equipped with a camera (model DFC360 FX; Leica). Images were taken at 60×, using Leica Application Suite 3.3.0. The following primary antibodies were used: γ-H2AX (#05-636; EMD Millipore), 53BP1 (sc-22760; Santa Cruz Biotechnology, Inc.), CycE (sc-198; Santa Cruz Biotechnology, Inc.), Cdc25A (sc-7389; Santa Cruz Biotechnology, Inc.), CHK1 pS345 (#2348; Cell Signaling Technology), CHK1 (sc-8408; Santa Cruz Biotechnology, Inc.), KAP1-pS824 (A300-767A; Bethyl Laboratories, Inc.), KAP1 (A300-274A; Bethyl Laboratories, Inc.), TFIIH (sc-293; Santa Cruz Biotechnology, Inc.), MUS81 (M1445; Sigma-Aldrich), and β-tubulin (sc-5274; Santa Cruz Biotechnology, Inc.). Secondary antibodies were Alexa Fluor conjugates (Alexa Fluor 488, 594, and 647; Invitrogen).
DNA fiber spreadings
Cells were sequentially pulse-labeled with 30 µM IdU and 250 µM CldU for 20 min each and harvested. Cells were then lysed and DNA fibers stretched onto glass slides, as described previously (Ray Chaudhuri et al., 2012). In brief, the fibers were denatured with 2.5 M HCl for 1 h, washed with PBS, and blocked with 0.2% Tween 20 in 2% BSA/PBS. CldU and IdU tracks were revealed with anti-BrdU antibodies recognizing CldU (ab6326; Abcam) and IdU (347580; BD), respectively, and appropriate secondary antibodies. Images were acquired with a microscope (model IX81; Olympus), CellR software (Olympus), and an Orca camera (Hamamatsu Photonics). Statistical analysis was performed using Prism (GraphPad Software).
Electron microscopic analysis of genomic DNA
In vivo psoralen cross-linking, isolation of total genomic DNA, and enrichment of the replication intermediates and their EM visualization were performed as described previously (Ray Chaudhuri et al., 2012; Neelsen et al., 2013). In brief, cells were harvested, and genomic DNA was cross-linked by two rounds of incubation in 10 µM 4,5′,8-trimethylpsoralen and 2 min of irradiation with 366-nm UV light. Cells were lysed, and genomic DNA was isolated from the nuclei by proteinase K digestion and phenol-chloroform extraction. Purified DNA was digested with PvuII and replication intermediates were enriched on a BND cellulose column. EM samples were prepared by spreading the DNA on carbon-coated grids and visualized by platinum rotary shadowing. Images were acquired on a microscope (G2 Spirit; FEI Tecnai) and analyzed with ImageJ (National Institutes of Health). Statistical analysis was performed using Prism.
Online supplemental material
Fig. S1 shows reversed replication forks and replication intermediates with exposed ssDNA observed upon OE of Cdc25A and CycE, and statistics on the accumulation of ssDNA regions under these conditions. Fig. S2 shows replication fork slow-down, accumulation of unusual replication intermediates, DSB formation, and DDR activation upon OE of Cdc25A and CycE in MRC5 cells. Fig. S3 illustrates the different H2AX phosphorylation patterns detected upon oncogene OE in FACS and single-cell IF, shows IF data on DDR markers (γ-H2AX and 53BP1), and accumulation of micronuclei and cells with ≥4n DNA in oncogene-overexpressing cells, FACS profiles for samples in Fig. 4, and the effect of ATR inhibition on CycE-overexpressing cells.
We are grateful to E. Petermann for sharing information before publication and to S. Ferrari and all members of the Lopes laboratory for helpful discussions. We thank O. Fernandez-Capetillo and J. Lukas for reagents, and the Center for Microscopy and Image Analysis of the University of Zurich for technical assistance.
This work was supported by Swiss National Science Foundation grants (PP0033-114922, PP00P3-1352), Krebsliga Zurich, and Research Priority Program on Systems Biology of the University of Zurich.
DNA damage response
DNA double-strand break
pulse-field gel electrophoresis
reversed replication fork
I.M.Y. Zanini’s present address is ETH Zurich, Institute of Biochemistry, Schafmattstrasse 18, 8093 Zurich, Switzerland.