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Cover Image
Cover Image
On the cover
A scanning electron micrograph shows a human monocyte attached to a VCAM-1–coated surface under fluid flow. Rullo et al. reveal that these cells resist detachment by rapidly polymerizing actin within an upstream structure that anchors them to the underlying substrate.
Image courtesy of Jacob Rullo and Henry Hong.
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In This Issue
In Focus
Big enough for two
Study identifies a signaling pathway that may control cell size by linking membrane transport to mitotic entry.
People & Ideas
Ewald Weibel: An organelle of his very own, and more
Weibel built his career using electron microscopy to study cells, tissues, and biological systems.
Review
Report
Nucleophosmin/B23 activates Aurora A at the centrosome through phosphorylation of serine 89
Aurora A, which is known to be activated by autophosphorylation at Thr288, is also locally activated during centrosomal maturation by nucleophosmin-mediated phosphorylation at Ser89.
Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell
Autophagy genes are not only essential for exposing the engulfment signal on apoptotic cells but also function in phagocytes to promote apoptotic cell removal.
Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy
After fertilization, the metalloendoprotease ovastacin is released by cortical granule exocytosis and cleaves the zona pellucida glycoprotein ZP2, an essential step to block sperm binding to an already fertilized egg.
Article
Nuclear translation visualized by ribosome-bound nascent chain puromycylation
A new method for visualizing translation in cells via standard immunofluorescence microscopy provides evidence for translation in the nucleoplasm and nucleolus.
An extended γ-tubulin ring functions as a stable platform in microtubule nucleation
Microtubule nucleation sites in yeast consist of a ring of γ-tubulin small complexes and a slight excess of uncomplexed γ-tubulin.
Netrin instructs synaptic vesicle clustering through Rac GTPase, MIG-10, and the actin cytoskeleton
A signaling module known to organize the actin cytoskeleton during neuronal guidance also instructs synaptic vesicle clustering during synapse assembly.
A link between mitotic entry and membrane growth suggests a novel model for cell size control
The strength of a signal generated by membrane growth could indicate when sufficient cell growth has occurred for mitotic entry.
Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism
Migrating fibroblasts reorient directionality by PI3K-dependent branching and pivoting of protrusions, a mechanism that allows fibroblasts to align with an external chemotactic gradient.
Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion
Rapid signaling and structural adaptations to the actin cytoskeleton enable leukocytes to stabilize α4 integrin–mediated adhesion and resist detachment from inflamed endothelium.
Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor
The crystal structure of the α5β1 integrin reveals conformational changes and amino acids important for ligand binding.
Tools
Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles
A multivariate proteomics approach identified numerous new clathrin-coated vesicle proteins as well as the first AP-4 accessory protein, and also revealed how auxilin depletion causes mitotic arrest through sequestration of spindle proteins in clathrin cages.
