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Cover Image
Cover Image
On the cover
A hair follicle (stained for E-cadherin, green) from a mouse whose skin lacks Runx1 in its mesenchymal layer shows ectopic expression of the transcription factor Lef1 (red) in the hair follicle stem cell compartment. Osorio et al. reveal that mesenchymal Runx1 suppresses epithelial Lef1 expression during embryonic development to guide the specification and maintenance of adult hair follicle stem cells. Image courtesy of Karen Osorio.
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In This Issue
In Focus
People & Ideas
Peter Walter: Investigating how the ER handles secretory proteins
Walter studies how proteins are targeted to the endoplasmic reticulum and how cells deal with ER stress.
Review
Report
Modulation of chromatin position and gene expression by HDAC4 interaction with nucleoporins
The histone deacetylase HDAC4 associates with the nuclear pore complex component Nup155 to modulate gene expression during cardiomyocyte hypertrophy.
Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice
Mouse NEAT1 is required for paraspeckle formation in a subset of cells but is not essential for animal health and viability.
Attenuation of yeast UPR is essential for survival and is mediated by IRE1 kinase
Mutations that impair activity of the ER stress response kinase Ire1 inhibit resolution of the unfolded protein response (see also a related paper by Rubio et al. in this issue).
ARH cooperates with AP-1B in the exocytosis of LDLR in polarized epithelial cells
The low-density lipoprotein receptor (LDLR) doesn’t directly bind AP-1B; however, it relies on this clathrin adaptor for basolateral exocytosis. Identification of the autosomal recessive hypercholesterolemia protein (ARH) as a link between LDLR and AP-1B explains this apparent discrepancy and provides a model for how other endocytic proteins may contribute to endosomal recycling.
Integrins traffic rapidly via circular dorsal ruffles and macropinocytosis during stimulated cell migration
In response to growth factor stimulation, integrins transit through recycling endosomes to reach newly forming focal adhesions at the cell’s leading edge.
Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis
Rings lost fulfills a novel function in Drosophila development for a ubiquitin receptor as an essential mediator of ring canal growth during oogenesis.
Article
HP1α recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair
p150CAF-1-mediated recruitment of HP1α to DNA is essential for efficient assembly of DNA damage response complexes and subsequent homologous recombination repair.
Replication stress induces 53BP1-containing OPT domains in G1 cells
53BP1-OPT domains, nuclear bodies that arise in G1 cells at sites of DNA damage induced by incomplete DNA replication, preferentially localize to chromosomal common fragile sites.
System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics
The ER–inner nuclear membrane trafficking of 15 integral membrane proteins followed by FRAP shows distinct ATP- and Ran-dependent translocation mechanisms.
Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins
The N and C termini of CENP-T undergo tension-dependent separation, suggesting that CENP-T elongation is responsible for changes in the shape of the inner kinetochore.
A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells
Knockdown or mislocalization of LGN complex components disrupts the stereotypic biphasic spindle movements regulating planar cell division and neuroepithelial structure in chick embryos.
The chromosomal passenger complex and centralspindlin independently contribute to contractile ring assembly
In contrast to their sequential roles in midzone assembly, the CPC and centralspindlin act through independent mechanisms to regulate contractile ring assembly.
Homeostatic adaptation to endoplasmic reticulum stress depends on Ire1 kinase activity
Uncoupling of Ire1’s RNAse and kinase activities reveals that its auto-phosphorylation is important for resolution of the unfolded protein response. (See also a related paper by Chawla et al. in this issue).
Munc18c phosphorylation by the insulin receptor links cell signaling directly to SNARE exocytosis
SNARE complex assembly and mobilization of GLUT4 vesicles is coordinated through direct targeting of Munc18c by the insulin receptor tyrosine kinase.
A presynaptic endosomal trafficking pathway controls synaptic growth signaling
Association of Nwk with SNX16 promotes down-regulation of synaptic growth signaling at the interface between early and recycling endosomes.
A hierarchy of signals regulates entry of membrane proteins into the ciliary membrane domain in epithelial cells
The NHERF1-ERM-actin network comprises a selective retention matrix that prevents interacting membrane proteins from entering the ciliary membrane.
Runx1 modulates adult hair follicle stem cell emergence and maintenance from distinct embryonic skin compartments
Runx1 controls timing of adult HSFC and short-term progenitor emergence in the embryonic epithelium and HFSC differentiation and long-term skin integrity in embryonic mesenchyme.
