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Cover Image
Cover Image
On the cover
West et al. use electron tomography to reconstruct the 3D organization of the ER in budding yeast and identify three peripheral ER domains: tubular ER (green), central cisternal ER (yellow) and plasma membrane–associated ER (blue). The image superimposes two separate tomograms from yeast with different bud diameters to demonstrate the ER domains' distributions during inheritance. The nuclear envelope is colored orange, the Golgi purple, and vesicles pink and white.
Image courtesy of Matt West.
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In This Issue
In Focus
Repair defects put stem cells in a fix
Study reveals that mutations in DNA-dependent protein kinase block multiple DNA repair pathways.
People & Ideas
Angelika Amon: Conquering the divide
Amon studies how cells segregate their chromosomes and what happens when they get it wrong.
Review
Report
MDC1 collaborates with TopBP1 in DNA replication checkpoint control
The DNA damage checkpoint protein MDC1 also interacts with TopBP1 to promote DNA replication checkpoint control.
Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells
Impaired autophagy stabilizes p62 and promotes tumorigenesis through activation of the Nrf2 transcription factor.
Chromosome passenger complexes control anaphase duration and spindle elongation via a kinesin-5 brake
Chromosome passenger complexes and bipolar kinesins act together to coordinate spindle elongation, spindle breakdown, and mitotic exit.
Article
Congenital bone marrow failure in DNA-PKcs mutant mice associated with deficiencies in DNA repair
Phosphorylation of DNA-PKcs is essential for activation of DNA damage repair and for the maintenance of tissue stem cell populations.
Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins
Centrin-null cells undergo normal division but are highly sensitive to UV irradiation as a result of impaired DNA repair.
Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling
In the absence of Tara, Trio binds to E-cadherin and increases activation of the E-cadherin transcriptional repressor Tbx3.
A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature
Electron tomography of continuous ER domains during budding shows that reticulons and Yop1 stabilize rather than generate membrane curvature in this organelle.
Isoform-specific targeting of PKA to multivesicular bodies
PKA RIα subunit is localized to MVBs by the A-kinase–anchoring protein AKAP11 when disassociated from the PKA catalytic subunit.
Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension
The cytoplasmic concentration of G-actin is a critical parameter for determining the extent of stimulus-induced G-actin assembly and cell extension.
Myosin IIA/IIB restrict adhesive and protrusive signaling to generate front–back polarity in migrating cells
Myosin IIA and IIB synergistically generate front–back polarity through their effects on actomyosin bundling formation and stability, and adhesion maturation, which are mediated by localized Rac GEF depletion.
Cdk2 loss accelerates precursor differentiation and remyelination in the adult central nervous system
Cdk2 is not essential for oligodendrocyte maturation and myelination during development, but in response to demyelination, it is required for oligodendrocyte precursor cell proliferation (OPC), and its loss accelerates OPC differentiation and remyelination.
miR-22 represses cancer progression by inducing cellular senescence
The microRNA miR-22 targets CDK6, SIRT1, and Sp1—genes involved in regulation of the senescence program—to suppress cell growth and proliferation.
