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Cover Image
Cover Image
On the cover
In the notum of a Drosophila pupa, dividing cells express a version of the Notch receptor-binding protein Sanpodo tagged with both GFP (green) and mCherry (magenta). The signals from these two fluorochromes do not overlap and thus reveal two distinct populations of Sanpodo within the cells. By following the transfer of dual-tagged Sanpodo from one subpopulation to the other, Couturier et al. reveal that the endocytic regulator Numb downregulates Notch signaling in pIIb cells by sorting Sanpodo to late endosomes and lysosomes.
Image © 2014 Couturier et al.
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In This Issue
In Focus
Red and green traffic signals
Dual-tagged proteins give a more complete picture of Notch receptor trafficking pathways.
People & Ideas
Dan Fletcher: A recipe for cooking up cellular machines
Fletcher specializes in reconstituting subcellular structures to test their behavior.
Review
Article
Repetitive centromeric satellite RNA is essential for kinetochore formation and cell division
SAT III RNA binds to the kinetochore component CENP-C and is required for correct assembly and function of the kinetochore at centromeres.
A fluorescent tagging approach in Drosophila reveals late endosomal trafficking of Notch and Sanpodo
Real-time analysis of fluorescently tagged versions of Notch and its trafficking regulator Sanpodo identify distinct pools of the two proteins and reveal a Numb-dependent sorting of Sanpodo to late endosomes.
Glucose uptake in brown fat cells is dependent on mTOR complex 2–promoted GLUT1 translocation
β3-Adrenoceptors promote glucose uptake in brown adipose tissue via both cAMP-mediated increases in GLUT1 transcription and mTORC2-stimulated translocation of newly synthesized GLUT1 to the plasma membrane.
Clarin-1 acts as a modulator of mechanotransduction activity and presynaptic ribbon assembly
The Usher syndrome type 3–associated protein clarin-1 is functionally important for mechanotransduction channel activity and for proper localization of synaptic components in zebrafish hair cells.
Tools
A method for multiprotein assembly in cells reveals independent action of kinesins in complex
A new system for generating cellular protein assemblies of defined spacing and composition reveals that kinesin motors located near each other function independently rather than cooperatively and are influenced primarily by the characteristics of the microtubule track on which they are moving.
Live cell micropatterning reveals the dynamics of signaling complexes at the plasma membrane
The use of micropatterned surfaces that bind HaloTag fusion proteins allows spatial organization of plasma membrane proteins for efficient visualization and quantification of protein–protein interactions in live cells.
pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis
A new pH-sensitive red fluorescent protein called pHuji, in combination with green fluorescent superecliptic pHluorin, allows two-color detection of endocytic events in live cells.
