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Cover Image
Cover Image
On the cover
Werner et al. describe how the Par complex and the microtubule-stabilizing protein CLAMP promote the radial intercalation of multiciliated cells into the skin of Xenopus embryos. Wild-type cells with multiple cilia (green) fully intercalate into the outer epithelium (labeled with phalloidin, red), whereas cells lacking CLAMP (blue) are trapped beneath the surface.
Image © 2014 Werner et al.
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In This Issue
In Focus
CLAMPing down on microtubules in migratory cells
Par complex and “sperm” protein crucial for embryonic cell relocation.
People & Ideas
Buzz Baum: The art of cell shape
Baum studies how cell shape affects tissue development, homeostasis, and cancer.
Review
Report
EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step
All three mammalian EDEM family members possess mannosidase activity and are necessary for glycoprotein degradation, but EDEM2 performs a unique, rate-limiting, first mannose trimming step upstream of EDEM1 and EDEM3.
A sterol-enriched vacuolar microdomain mediates stationary phase lipophagy in budding yeast
During stationary phase growth in yeast, a feed-forward loop exists in which lipophagy stimulates vacuolar microdomain formation, which, in turn, further promotes lipophagy.
Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1
During radial intercalation of epithelial cells, Par3 and aPKC promote the apical positioning of centrioles, whereas CLAMP stabilizes microtubules along the axis of migration.
Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology
Major changes in trypanosome cell form can be achieved by simple modulation of the calpain-like protein ClpGM6 via coordinated association and positioning of membrane and cytoskeletal components.
Article
TPX2 levels modulate meiotic spindle size and architecture in Xenopus egg extracts
TPX2 levels modulate spindle architecture through Eg5, partitioning microtubules between a tiled, antiparallel array that promotes spindle expansion and a cross-linked, parallel architecture that concentrates microtubules at spindle poles.
Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration
The focal adhesion scaffold protein paxillin coordinates microtubule acetylation-dependent cell polarization and migration in both normal and transformed cells through a direct inhibitory interaction with the α-tubulin deacetylase HDAC6.
PDK1-mediated activation of MRCKα regulates directional cell migration and lamellipodia retraction
MRCKα is activated by PDK1 through a PIP3-dependent, kinase-independent mechanism that drives the relocation of both proteins to lamellipodia and regulates lamellipodial retraction and directional migration.
Dynamic myosin phosphorylation regulates contractile pulses and tissue integrity during epithelial morphogenesis
Dynamic regulation of Myo-II by Rho kinase and myosin phosphatase organizes contractile Myo-II pulses in both space and time, which is necessary to maintain tissue integrity during morphogenesis.
