On the cover
Werner et al. describe how the Par complex and the microtubule-stabilizing protein CLAMP promote the radial intercalation of multiciliated cells into the skin of Xenopus embryos. Wild-type cells with multiple cilia (green) fully intercalate into the outer epithelium (labeled with phalloidin, red), whereas cells lacking CLAMP (blue) are trapped beneath the surface.
Image © 2014 Werner et al.
See page 367.
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All three mammalian EDEM family members possess mannosidase activity and are necessary for glycoprotein degradation, but EDEM2 performs a unique, rate-limiting, first mannose trimming step upstream of EDEM1 and EDEM3.
During stationary phase growth in yeast, a feed-forward loop exists in which lipophagy stimulates vacuolar microdomain formation, which, in turn, further promotes lipophagy.
Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1
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TPX2 levels modulate spindle architecture through Eg5, partitioning microtubules between a tiled, antiparallel array that promotes spindle expansion and a cross-linked, parallel architecture that concentrates microtubules at spindle poles.
Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration
The focal adhesion scaffold protein paxillin coordinates microtubule acetylation-dependent cell polarization and migration in both normal and transformed cells through a direct inhibitory interaction with the α-tubulin deacetylase HDAC6.
MRCKα is activated by PDK1 through a PIP3-dependent, kinase-independent mechanism that drives the relocation of both proteins to lamellipodia and regulates lamellipodial retraction and directional migration.
Dynamic myosin phosphorylation regulates contractile pulses and tissue integrity during epithelial morphogenesis
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