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Cover Image
Cover Image
On the cover
Fernandes and Schöck reveal that Lasp, a much shorter version of the vertebrate giant protein nebulin, regulates the architecture of Drosophila muscle sarcomeres. Compared to a wild-type myofibril (top), a myofibril from the indirect flight muscle of a fly lacking Lasp (bottom) has shorter, misaligned thin filaments (magenta), and the titin family protein kettin (green) shows a wider distribution around each I-band.
Image © 2014 Fernandes and Schöck.
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In This Issue
In Focus
Vps74 gives phosphatase directions
Study reveals how the Sac1 phosphatase removes PtdIns4P from early Golgi membranes.
People & Ideas
Heidi McBride: Mitochondria are well connected
McBride studies mitochondrial dynamics and mitochondrial trafficking with other organelles.
Review
Report
cPKC regulates interphase nuclear size during Xenopus development
During Xenopus development, increased nuclear cPKC activity and decreased nuclear association of lamins mediate nuclear scaling.
Sac1–Vps74 structure reveals a mechanism to terminate phosphoinositide signaling in the Golgi apparatus
Characterization of the structure of the N-terminal portion of yeast Sac1 in complex with the phosphatidylinositol 4-kinase effector Vps74 identifies how Sac1 is directed to dephosphorylate PtdIns4P in the Golgi.
Article
Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery
Phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress.
A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A
During kinetochore assembly in budding yeast, the key steps of CENP-A recognition and outer kinetochore recruitment are executed through different yeast CCAN subunits, potentially protecting against inappropriate kinetochore assembly.
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins
The oxidoreductases activity of MagT1, an ER-localized thioredoxin homologue, is necessary for posttranslocational N-glycosylation of acceptor sites that lie proximal to cysteine residues.
High density and ligand affinity confer ultrasensitive signal detection by a guanylyl cyclase chemoreceptor
The sea urchin sperm guanylyl cyclase chemoreceptor achieves ultrasensitive signal detection and precise signal modulation through high receptor density, subnanomolar ligand affinity, and sequential dephosphorylation.
The nebulin repeat protein Lasp regulates I-band architecture and filament spacing in myofibrils
With just two nebulin repeats, the Drosophila protein Lasp controls muscle thin filament length and filament spacing.
