On the cover
An artistically rendered image showing the spatial separation of endocytic pathways in a migrating fibroblast. Clathrin-coated vesicles (blue) are nonpolarized, while clathrin-independent carriers (red) localize to the leading edge and caveolae (green) form at the rear of the cell. Howes et al. reveal that clathrin-independent internalization is the predominant endocytic pathway in fibroblasts.
See page 675.
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Cyclin A outcompetes inhibitory spindle assembly checkpoint proteins for binding to the APC/C ubiquitin ligase coactivator Cdc20 to promote its self-destruction even when the checkpoint is active (see also a paper from van Zon et al., in this issue).
Generation of PI3P in the normally PI3P-deficient ER membrane makes the organelle a platform for autophagosome formation.
Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis
Blocking autophagy protects the apoptosis inhibitor dBruce from destruction and promotes nurse cell survival in developing egg chambers.
Identification of novel filament-forming proteins in Saccharomyces cerevisiae and Drosophila melanogaster
A screen for GFP-tagged yeast proteins that can assemble into visible structures reveals four new filamentous structures in the cytoplasm formed by metabolic enzymes and translation factors.
A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA
ERK8 prevents genome instability by blocking the association of the HDM2 E3 ubiquitin ligase with the genome-protecting protein PCNA.
The APC/C recruits cyclin B1–Cdk1–Cks in prometaphase before D box recognition to control mitotic exit
Prior associations with the APC/C complex during prometaphase makes cyclin B1 a better substrate for the cell cycle–regulating ubiquitin ligase in metaphase (see also a related paper by Di Fiore et al. in this issue).
Passage of transcribed U snRNA precursors through Cajal bodies ensures that they are properly bound to the PHAX adaptor protein required for nuclear exit.
CryoET shows the configuration of the ephemeral translationally inactive 100S ribosomal dimer.
Lipid activation of the signal recognition particle receptor provides spatial coordination of protein targeting
Phospholipid binding leads to accelerated assembly of the bacterial SRP receptor FtsY and SRP, allowing cargo proteins to be delivered to target membranes more efficiently.
The ubiquitin-like protein BAG-6 protects cells from newly synthesized misfolded proteins by tethering them to the proteasome.
p107, a member of the retinoblastoma susceptibility protein family, influences lipid metabolism in muscle and other tissues by repressing the PPAR-γ co-factor PGC-1α.
Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
Quantitative ultrastructural analysis and proteomics detail CLIC structure, composition, and function.
In response to tissue stiffening, fibroblasts increase production of extracellular matrix while decreasing production of matrix-degrading enzymes and the fibrosis inhibitor prostaglandin E2.