-
Cover Image
Cover Image
On the cover
Oscillating contractions of polar cortical myosin (green) rocks the mitotic spindle (red) back and forth in cells with abnormally long astral microtubules or—as pictured here—cells lacking the cleavage furrow protein anillin.
See page 35. - PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In This Issue
In Focus
Sar1 bends membranes into shape
The small GTPase forms an organized scaffold that can regulate the pinching of vesicles from the ER.
People & Ideas
David Pellman: Grasping the geometry of cancer
David Pellman investigates the constraints and advantages that alteration of chromosome number places on dividing cells.
Editorial
Review
Report
Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1–C-Mad2 core complex
To satisfy the mitotic checkpoint and drive chromosome congression, the Mps1 kinase lets go of kinetochores by phosphorylating itself in trans (see also related papers by Maciejowski et al. and Santaguida et al. in this issue).
Long astral microtubules uncouple mitotic spindles from the cytokinetic furrow
Depletion of MCAK, which lengthens astral microtubules, induces oscillations of the mitotic spindle, and displaces the plane of cell division.
The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex
The PAFAH 1b complex links phospholipid remodeling and membrane tubulation within the Golgi to dynein-dependent transport.
SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion
Whittling away SNARE complex components reveals essential domains for Munc18-1–mediated membrane fusion.
Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins
Mot48, a PIH domain protein, assembles and stabilizes inner arm dynein complexes in the cytoplasm before they are transported into cilia.
Article
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
Addition of reversine to dividing cells ejects Mad1 and the RZZ complex from unattached kinetochores and prevents resolution of incorrect chromosome–microtubule attachments (see also related papers by Hewitt et al. and Maciejowski et al. in this issue).
Mps1 directs the assembly of Cdc20 inhibitory complexes during interphase and mitosis to control M phase timing and spindle checkpoint signaling
Cdc20 and Mad2 or Bub1 don’t come together in Mps1-null cells, resulting in a dramatic acceleration of anaphase onset (see also related papers by Hewitt et al. and Santaguida et al. in this issue).
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A
In addition to its function as an Arp2/3 complex subunit, Arp1cb interacts with and stimulates Aurora A at centrosomes, functioning in cell cycle progression.
Sar1 assembly regulates membrane constriction and ER export
While dynamin pinches vesicles from the plasma membrane, the Sar1 GTPase specializes in cinching ER membrane tubules.
The SPRY domain–containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation
Macrophages lacking SPSB2 have increased NO production and enhanced pathogen-killing capabilities due to decreased ubiquitin-mediated destruction of iNOS.
Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1
The Chlamydia-encoded protein Tarp is phosphorylated in the host cell cytoplasm and is a multivalent hub for antiapoptotic signaling molecules.
