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Cover Image
Cover Image
On the cover
TIRF microscopy reveals that clathrin (blue) and its adaptor protein Dab2 (green) colocalize at focal adhesions (red), which are disassembled by the endocytosis of integrins.
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In This Issue
In Focus
People & Ideas
Frauke Melchior: How SUMO wrestles other proteins
Melchior's laboratory is exploring the regulation and consequences of protein modification by the small protein SUMO.
Review
Report
Separating the spindle, checkpoint, and timer functions of BubR1
The BubR1 kinase domain controls spindle attachment to the kinetochores, whereas the KEN domain regulates activation of the spindle assembly checkpoint.
Centrosomal Aki1 and cohesin function in separase-regulated centriole disengagement
Cohesin subunit Scc1 and Akt kinase–interacting protein 1 both localize to centrosomes and regulate the timing of centriole separation during mitosis.
Kinesin 9 family members perform separate functions in the trypanosome flagellum
KIF9B localizes to the axoneme and basal body and is needed for flagella assembly, whereas KIF9A localizes only to the axoneme and controls flagella motility without affecting their structure.
Article
Decreased replication origin activity in temporal transition regions
Experimental attempts to activate replication origins within the temporal transition region in the IgH locus in mouse embryonic stem cells were not successful, and thus, why and how they become activated in B cells remains unclear.
INCENP–aurora B interactions modulate kinase activity and chromosome passenger complex localization
INCENP fine tunes the level of aurora B kinase activity, which in turn correlates with different functional states of the chromosome passenger complex.
Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates
An E2–E3 complex can ubiquitinate substrates via either an isopeptide bond (to a lysine) or an ester bond (to a serine or threonine) and preferentially uses the latter to induce ERAD.
The glycosaminoglycan-binding domain of PRELP acts as a cell type–specific NF-κB inhibitor that impairs osteoclastogenesis
The PRELP heparin sulfate–binding protein translocates to the nucleus, where it impairs NF-κB transcriptional activity, which in turn regulates bone homeostasis.
δ-EF1 is a negative regulator of Ihh in the developing growth plate
Hedgehog protein IHH expression is regulated by transcription factor δ-EF1 to control endochondral bone formation
An electrostatic switch displaces phosphatidylinositol phosphate kinases from the membrane during phagocytosis
PIP5K is held at the membrane of forming phagosomes by a conserved, positively charged patch. During particle engulfment, the surface charge of the phagosome decreases, releasing PIP5K and enabling phagocytosis to proceed.
New PI(4,5)P2- and membrane proximal integrin–binding motifs in the talin head control β3-integrin clustering
A talin intermolecular interaction autoinhibits its own activation and regulates β3-integrin binding. When bound, β3-integrin undergoes structural alterations that prevent its β and α subunits from associating, maintaining β3-integrin's clustering capability.
Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells
A new mechanism for focal adhesion disassembly is described involving microtubule-stimulated endocytosis of integrins at focal adhesions.
