Detection of Ca2+ transients near ryanodine receptors by targeting fluorescent Ca2+ sensors to the triad
Sanchez et al. target the Ca2+-sensitive dye CGaMP6f to the triad region of the sarcomere by using the T306 domain of triadin. Their approach reveals synchronous Ca2+ release across individual triads and will facilitate the study of ryanodine receptor channel function.
Fast inactivation of Nav current in rat adrenal chromaffin cells involves two independent inactivation pathways
Martinez-Espinosa et al. show that fast and slow recovery of voltage-dependent Na+ (Nav) channels in rat adrenal chromaffin cells depends on two independent inactivation pathways. Competition between these pathways regulates changes in Nav channel availability.
Not so transport incompetent after all: Revisiting a CLC-7 mutant sheds new mechanistic light on lysosomal physiology
Accardi reviews data on the lysosomal 2 Cl−/1 H+ antiporter ClC-7.
A new structure of a CLC antiporter mutant, along with EPR spectroscopy and computational dynamics, now resolves several basic puzzles regarding how these transporters stoichiometrically move Cl− and H+ in opposite directions across biological membranes.