This review outlines a holistic framework for studying ATP synthase and emphasizes the importance of considering interactions with the lipid environment in shaping the function and evolutionary history of membrane proteins.
Determinants of Ca2+ release restitution: Insights from genetically altered animals and mathematical modeling
The present work shows that SR Ca2+ load and RYR2 Ca2+ sensitivity are major determinants of Ca2+ release restitution (CRR) after each heartbeat. Mathematical modeling led us to speculate that the velocity of SR Ca2+ refilling might regulate CRR independently of SR Ca2+ content.
Prieto et al. describe how ultrasound can either inhibit or potentiate action potential firing in hippocampal pyramidal neurons and demonstrate that these effects can be explained by increased potassium conductance.
Methods and Approaches
A novel voltage-clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels
Gaete et al. have designed a methodology that allows quantitative analysis of the permeation of ions and molecules through connexin and CALHM1 large-pore channels. Both channels display saturable transport of molecules that could be described by Michaelis-Menten kinetics with apparent Km and Vmax.