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Journal Articles

Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology

Takahiro K. Fujiwara, Shinji Takeuchi, Ziya Kalay, Yosuke Nagai, Taka A. Tsunoyama, Thomas Kalkbrenner, Kokoro Iwasawa, Ken P. Ritchie, Kenichi G.N. Suzuki, Akihiro Kusumi

J Cell Biol (2023) 222 (8): e202110160.

https://doi.org/10.1083/jcb.202110160

Published: 06 June 2023

View article titled, Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology

Journal Articles

Ultrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamics

In Special Collection:

Mechanobiology 2023

Takahiro K. Fujiwara, Taka A. Tsunoyama, Shinji Takeuchi, Ziya Kalay, Yosuke Nagai, Thomas Kalkbrenner, Yuri L. Nemoto, Limin H. Chen, Akihiro C.E. Shibata, Kokoro Iwasawa, Ken P. Ritchie, Kenichi G.N. Suzuki, Akihiro Kusumi

J Cell Biol (2023) 222 (8): e202110162.

https://doi.org/10.1083/jcb.202110162

Published: 06 June 2023

View article titled, Ultrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamics

Journal Articles

High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

Ikuko Koyama-Honda, Takahiro K. Fujiwara, Rinshi S. Kasai, Kenichi G.N. Suzuki, Eriko Kajikawa, Hisae Tsuboi, Taka A. Tsunoyama, Akihiro Kusumi

J Cell Biol (2020) 219 (12): e202006125.

https://doi.org/10.1083/jcb.202006125

Published: 14 October 2020

View article titled, High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

Includes: Supplementary data

Journal Articles

Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs

Masanao Kinoshita, Kenichi G.N. Suzuki, Nobuaki Matsumori, Misa Takada, Hikaru Ano, Kenichi Morigaki, Mitsuhiro Abe, Asami Makino, Toshihide Kobayashi, Koichiro M. Hirosawa, Takahiro K. Fujiwara, Akihiro Kusumi, Michio Murata

J Cell Biol (2017) 216 (4): 1183–1204.

https://doi.org/10.1083/jcb.201607086

Published: 22 March 2017

View article titled, Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs

Includes: Supplementary data

Journal Articles

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging

Hirohito Nishimura, Ken Ritchie, Rinshi S. Kasai, Miki Goto, Nobuhiro Morone, Hiroyuki Sugimura, Koichiro Tanaka, Ichiro Sase, Akihiko Yoshimura, Yoshitaro Nakano, Takahiro K. Fujiwara, Akihiro Kusumi

J Cell Biol (2013) 202 (6): 967–983.

https://doi.org/10.1083/jcb.201301053

Published: 16 September 2013

View article titled, Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging

Includes: Supplementary data

Journal Articles

Full characterization of GPCR monomer–dimer dynamic equilibrium by single molecule imaging

Rinshi S. Kasai, Kenichi G. N. Suzuki, Eric R. Prossnitz, Ikuko Koyama-Honda, Chieko Nakada, Takahiro K. Fujiwara, Akihiro Kusumi

J Cell Biol (2011) 192 (3): 463–480.

https://doi.org/10.1083/jcb.201009128

Published: 07 February 2011

View article titled, Full characterization of GPCR monomer–dimer dynamic equilibrium by single molecule imaging

Includes: Supplementary data

Journal Articles

GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1

Kenichi G.N. Suzuki, Takahiro K. Fujiwara, Fumiyuki Sanematsu, Ryota Iino, Michael Edidin, Akihiro Kusumi

J Cell Biol (2007) 177 (4): 717–730.

https://doi.org/10.1083/jcb.200609174

Published: 21 May 2007

View article titled, GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1

Includes: Supplementary data

Journal Articles

Dynamic recruitment of phospholipase Cγ at transiently immobilized GPI-anchored receptor clusters induces IP₃–Ca²⁺ signaling: single-molecule tracking study 2

Kenichi G.N. Suzuki, Takahiro K. Fujiwara, Michael Edidin, Akihiro Kusumi

J Cell Biol (2007) 177 (4): 731–742.

https://doi.org/10.1083/jcb.200609175

Published: 21 May 2007

View article titled, Dynamic recruitment of phospholipase Cγ at transiently immobilized GPI-anchored receptor clusters induces IP<sub>3</sub>–Ca<sup>2+</sup> signaling: single-molecule tracking study 2

Includes: Supplementary data

Journal Articles

Microtubules and the endoplasmic reticulum are highly interdependent structures.

M Terasaki, L B Chen, K Fujiwara

J Cell Biol (1986) 103 (4): 1557–1568.

https://doi.org/10.1083/jcb.103.4.1557

Published: 01 October 1986

View article titled, Microtubules and the endoplasmic reticulum are highly interdependent structures.

Journal Articles

Expression and intracellular distribution of stress fibers in aortic endothelium.

G E White, K Fujiwara

J Cell Biol (1986) 103 (1): 63–70.

https://doi.org/10.1083/jcb.103.1.63

Published: 01 July 1986

View article titled, Expression and intracellular distribution of stress fibers in aortic endothelium.

Journal Articles

Improved fixation for immunofluorescence microscopy using light-activated 1,3,5-triazido-2,4,6-trinitrobenzene (TTB).

E McBeath, K Fujiwara

J Cell Biol (1984) 99 (6): 2061–2073.

https://doi.org/10.1083/jcb.99.6.2061

Published: 01 December 1984

View article titled, Improved fixation for immunofluorescence microscopy using light-activated 1,3,5-triazido-2,4,6-trinitrobenzene (TTB).

Journal Articles

Myosin in cultured vascular smooth muscle cells: immunofluorescence and immunochemical studies of alterations in antigenic expression.

D M Larson, K Fujiwara, R W Alexander, M A Gimbrone, Jr

J Cell Biol (1984) 99 (5): 1582–1589.

https://doi.org/10.1083/jcb.99.5.1582

Published: 01 November 1984

View article titled, Myosin in cultured vascular smooth muscle cells: immunofluorescence and immunochemical studies of alterations in antigenic expression.

Journal Articles

Factors influencing the expression of stress fibers in vascular endothelial cells in situ.

G E White, M A Gimbrone, Jr, K Fujiwara

J Cell Biol (1983) 97 (2): 416–424.

https://doi.org/10.1083/jcb.97.2.416

Published: 01 August 1983

View article titled, Factors influencing the expression of stress fibers in vascular endothelial cells in situ.

Journal Articles

Distribution of alpha-actinin in single isolated smooth muscle cells.

F S Fay, K Fujiwara, D D Rees, K E Fogarty

J Cell Biol (1983) 96 (3): 783–795.

https://doi.org/10.1083/jcb.96.3.783

Published: 01 March 1983

View article titled, Distribution of alpha-actinin in single isolated smooth muscle cells.

Journal Articles

Organization of actin, myosin, and intermediate filaments in the brush border of intestinal epithelial cells.

N Hirokawa, L G Tilney, K Fujiwara, J E Heuser

J Cell Biol (1982) 94 (2): 425–443.

https://doi.org/10.1083/jcb.94.2.425

Published: 01 August 1982

View article titled, Organization of actin, myosin, and intermediate filaments in the brush border of intestinal epithelial cells.

Journal Articles

Stress fibers in cells in situ: immunofluorescence visualization with antiactin, antimyosin, and anti-alpha-actinin.

H R Byers, K Fujiwara

J Cell Biol (1982) 93 (3): 804–811.

https://doi.org/10.1083/jcb.93.3.804

Published: 01 June 1982

View article titled, Stress fibers in cells in situ: immunofluorescence visualization with antiactin, antimyosin, and anti-alpha-actinin.

Journal Articles

Characterization and localization of myosin in the brush border of intestinal epithelial cells

MS Mooseker, TD Pollard, K Fujiwara

J Cell Biol (1978) 79 (2): 444–453.

https://doi.org/10.1083/jcb.79.2.444

Published: 01 November 1978

View article titled, Characterization and localization of myosin in the brush border of intestinal epithelial cells

Journal Articles

Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. II. Contrasting effects of local anesthetics and a calcium ionophore.

J Braun, K Fujiwara, T D Pollard, E R Unanue

J Cell Biol (1978) 79 (2): 419–426.

https://doi.org/10.1083/jcb.79.2.419

Published: 01 November 1978

View article titled, Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. II. Contrasting effects of local anesthetics and a calcium ionophore.

Journal Articles

Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. I. Relationship to cytoplasmic myosin.

J Braun, K Fujiwara, T D Pollard, E R Unanue

J Cell Biol (1978) 79 (2): 409–418.

https://doi.org/10.1083/jcb.79.2.409

Published: 01 November 1978

View article titled, Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. I. Relationship to cytoplasmic myosin.

Journal Articles

Alpha-actinin localization in the cleavage furrow during cytokinesis.

K Fujiwara, M E Porter, T D Pollard

J Cell Biol (1978) 79 (1): 268–275.

https://doi.org/10.1083/jcb.79.1.268

Published: 01 October 1978

View article titled, Alpha-actinin localization in the cleavage furrow during cytokinesis.

NARROW

Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology

Ultrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamics

High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging

Full characterization of GPCR monomer–dimer dynamic equilibrium by single molecule imaging

GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1

Dynamic recruitment of phospholipase Cγ at transiently immobilized GPI-anchored receptor clusters induces IP₃–Ca²⁺ signaling: single-molecule tracking study 2

Microtubules and the endoplasmic reticulum are highly interdependent structures.

Expression and intracellular distribution of stress fibers in aortic endothelium.

Improved fixation for immunofluorescence microscopy using light-activated 1,3,5-triazido-2,4,6-trinitrobenzene (TTB).

Myosin in cultured vascular smooth muscle cells: immunofluorescence and immunochemical studies of alterations in antigenic expression.

Factors influencing the expression of stress fibers in vascular endothelial cells in situ.

Distribution of alpha-actinin in single isolated smooth muscle cells.

Organization of actin, myosin, and intermediate filaments in the brush border of intestinal epithelial cells.

Stress fibers in cells in situ: immunofluorescence visualization with antiactin, antimyosin, and anti-alpha-actinin.

Characterization and localization of myosin in the brush border of intestinal epithelial cells

Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. II. Contrasting effects of local anesthetics and a calcium ionophore.

Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. I. Relationship to cytoplasmic myosin.

Alpha-actinin localization in the cleavage furrow during cytokinesis.

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