On the cover
Thirty years after Kirschner and Mitchison first formulated the search-and-capture hypothesis, Heald and Khodjakov review how cells rapidly and accurately assemble the mitotic spindle. Fluorescence microscopy shows the spindle’s organization in a non-transformed human RPE1 cell during metaphase. Microtubules are labeled red, kinetochores (CENP-A-GFP) and centrioles (centrin-GFP) are green, and chromosomes are blue.
Image © 2015 Valentin Magidson et al.
See page 1103.
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People & Ideas
The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
The microtubule catastrophe-promoting complex Sentin-EB1 delays stable kinetochore–microtubule attachment and facilitates bipolar attachment of homologous chromosomes in Drosophila oocytes.
In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p
Single-particle imaging in budding yeast demonstrates that mRNP export is fast (∼200 ms) and that mRNPs are retained at NPCs and undergo retrograde transport in a mex67-5 mutant, proving an essential role for Mex67p in directional mRNP transport.
Single-molecule resolution particle tracking reveals that mRNAs in S. cerevisiae scan the nuclear periphery before being exported to the cytoplasm and that this process is mediated by both components of the nuclear basket and the mRNP.
Whole-proteome analysis of isolated mitotic chromosomes from 11 kinetochore structural and assembly mutants is used to develop dependency and correlation maps for protein subcomplexes that confirm many published interactions and also reveal novel dependencies between kinetochore components.
Physical and functional interaction between the α- and γ-secretases: A new model of regulated intramembrane proteolysis
The α-secretase ADAM10 interacts with γ-secretase in a proteolytically functional complex, which may suggest a new mechanism of RIP signaling where the sheddase and intramembrane protease reside together in a complex that can accept and process full-length substrates efficiently.
EPS8, a regulator of actin dynamics, is a novel component of the adherens junction complex of endothelial cells and acts as the key protein through which VE-cadherin controls Yap transcriptional activity both in vitro and in vivo.
Repair of plasma membrane wounds in B lymphocytes that lack caveolin requires lysosome exocytosis and lipid raft–mediated endocytosis and inhibits activation of the B cell receptor by sequestering lipid rafts.