On the cover
In an early Drosophila embryo stained for the nuclear envelope (green) and DNA (blue), hairy mRNA (magenta) accumulates in the apical cytoplasm. Dix et al. reveal that the Lissencephaly-1 protein promotes the recruitment of dynein and dynactin to hairy and other mRNA cargoes to facilitate their transport along microtubules.
Image © 2013 Dix et al.
See page 479.
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The juxtaposition of a double-stranded DNA end and a short single-stranded DNA gap triggers robust activation of endogenous ATR and Chk1 mediated by DNA-PKcs.
Microtubule severing by the katanin complex is activated by PPFR-1–dependent MEI-1 dephosphorylation
Dephosphorylation of MEI-1 activates katanin during meiosis, whereas ubiquitin-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation during mitosis.
Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
Tubulin glutamylating enzymes are important for beating behavior of ependymal cilia in the brain, whereas glycylating enzymes are critical for stability and maintenance of these cilia.
FRET-based sensors for cAMP and PKA activity reveal that mitochondrial subcompartments host segregated cAMP cascades with distinct functional and kinetic signatures.
Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding
Aurora A phosphorylation-induced interaction of TACC3 and clathrin coordinates adjacent domains in each protein to create a microtubule-binding interface, whereas a distinct site in TACC3 recruits ch-TOG to mitotic spindles.
Lissencephaly-1 promotes the interaction of dynein with dynactin and facilitates motor complex association with mRNA cargos.
Phosphorylation of the scaffolding protein JIP1 serves as a molecular switch to coordinate anterograde and retrograde microtubule motor complexes involved in amyloid precursor protein transport.
The exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins to confer both specificity and directionality to vesicular traffic.
Inclusion of IST1 into the ESCRT complex allows recruitment of the microtubule-severing protein spastin to promote fission of recycling tubules from the endosome.
CCM1–ICAP-1 complex controls β1 integrin–dependent endothelial contractility and fibronectin remodeling
Loss of CCM1/2 leads to destabilization of ICAP-1 and up-regulation of β1 integrin, resulting in the destabilization of intercellular junctions due to increased cell contractility and aberrant extracellular matrix remodeling.
Macrophages infiltrating the pancreas in response to inflammation induce cellular transdifferentiation by secreting cytokines that activate NF-κB signaling and matrix metalloproteinase expression.
A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair
A combination of RNase- and detergent-based preextraction with high-resolution microscopy allows the detection of Ku and other DNA repair proteins at single double-strand breaks in cells.