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Cover Image
Cover Image
On the cover
In an early Drosophila embryo stained for the nuclear envelope (green) and DNA (blue), hairy mRNA (magenta) accumulates in the apical cytoplasm. Dix et al. reveal that the Lissencephaly-1 protein promotes the recruitment of dynein and dynactin to hairy and other mRNA cargoes to facilitate their transport along microtubules.
Image © 2013 Dix et al.
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In This Issue
In Focus
The CCM complex puts a cap on integrin activity
Disease-related proteins regulate β1 integrin to limit cell contractility and maintain vascular integrity.
People & Ideas
Bob Goldstein: Cell biology by way of development
Goldstein is studying the cell biology of how organisms develop.
Review
Report
DNA structure-specific priming of ATR activation by DNA-PKcs
The juxtaposition of a double-stranded DNA end and a short single-stranded DNA gap triggers robust activation of endogenous ATR and Chk1 mediated by DNA-PKcs.
Microtubule severing by the katanin complex is activated by PPFR-1–dependent MEI-1 dephosphorylation
Dephosphorylation of MEI-1 activates katanin during meiosis, whereas ubiquitin-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation during mitosis.
Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
Tubulin glutamylating enzymes are important for beating behavior of ependymal cilia in the brain, whereas glycylating enzymes are critical for stability and maintenance of these cilia.
The inner and outer compartments of mitochondria are sites of distinct cAMP/PKA signaling dynamics
FRET-based sensors for cAMP and PKA activity reveal that mitochondrial subcompartments host segregated cAMP cascades with distinct functional and kinetic signatures.
Article
Coordination of adjacent domains mediates TACC3–ch-TOG–clathrin assembly and mitotic spindle binding
Aurora A phosphorylation-induced interaction of TACC3 and clathrin coordinates adjacent domains in each protein to create a microtubule-binding interface, whereas a distinct site in TACC3 recruits ch-TOG to mitotic spindles.
Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs
Lissencephaly-1 promotes the interaction of dynein with dynactin and facilitates motor complex association with mRNA cargos.
JIP1 regulates the directionality of APP axonal transport by coordinating kinesin and dynein motors
Phosphorylation of the scaffolding protein JIP1 serves as a molecular switch to coordinate anterograde and retrograde microtubule motor complexes involved in amyloid precursor protein transport.
The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p
The exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins to confer both specificity and directionality to vesicular traffic.
An ESCRT–spastin interaction promotes fission of recycling tubules from the endosome
Inclusion of IST1 into the ESCRT complex allows recruitment of the microtubule-severing protein spastin to promote fission of recycling tubules from the endosome.
CCM1–ICAP-1 complex controls β1 integrin–dependent endothelial contractility and fibronectin remodeling
Loss of CCM1/2 leads to destabilization of ICAP-1 and up-regulation of β1 integrin, resulting in the destabilization of intercellular junctions due to increased cell contractility and aberrant extracellular matrix remodeling.
Macrophage-secreted cytokines drive pancreatic acinar-to-ductal metaplasia through NF-κB and MMPs
Macrophages infiltrating the pancreas in response to inflammation induce cellular transdifferentiation by secreting cytokines that activate NF-κB signaling and matrix metalloproteinase expression.
Tools
A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair
A combination of RNase- and detergent-based preextraction with high-resolution microscopy allows the detection of Ku and other DNA repair proteins at single double-strand breaks in cells.
