On the cover
In a laser-wounded Drosophila pupal notum, epithelial cells are labeled with E-cadherin (green) and the actin-binding protein moesin (red). Antunes et al. investigate the early stages of epithelial wound closure, identifying a wave of actomyosin assembly and cell constriction that flows toward the wound edge.
Image © 2013 Antunes et al.
See page 365.
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This review discusses how kinetic proofreading by Rab GTPases provides a speed-dating mechanism defining the identity of membrane domains in vesicle trafficking.
Comprehensive analysis of the intranuclear territories and motion of budding yeast chromosome XII loci suggests that long-range chromosome architecture is mainly determined by the physical principles of polymers.
The slow increase in CDK1 activity in meiosis I acts as a timing mechanism to allow stable kinetochore–microtubule attachments only after bipolar spindle formation, thus preventing attachment errors.
The kinesin-5 motor Eg5 has a novel non-mitotic role in the transport of a specific class of transport carriers (CARTS) from the trans-Golgi network to the cell surface.
The direct interaction between the ERM protein Moesin and microtubules is required for spindle organization in metaphase and cell shape transformation after anaphase onset.
Chromatin insulator bodies are nuclear structures that form in response to osmotic stress and cell death
Insulator bodies are novel nuclear stress foci that can be used as a proxy to monitor the chromatin-bound state of insulator proteins.
Greatwall kinase relocation from the nucleus to the cytoplasm is required at mitotic entry and is mediated by a phosphorylation-dependent mechanism targeting its central region.
Loss of BubR1 acetylation causes defects in spindle assembly checkpoint signaling and promotes tumor formation
Failure of chromosome–spindle attachment and a weakened spindle assembly checkpoint lead to genetic instability and cancer in mice expressing acetylation-deficient BubR1.
Rho1 inactivation by Cyk3 promotes primary septum formation and cleavage-furrow ingression, whereas Rho1 activation or Cdc42 inactivation promotes secondary septum formation and abscission.
SNX18 promotes autophagosome formation by remodeling membranes and providing membrane to forming autophagosomes.
Kinesin-1–syntaphilin coupling mediates activity-dependent regulation of axonal mitochondrial transport
Syntaphilin mediates the activity-dependent immobilization of axonal mitochondria by physically displacing KIF5 from the Miro–Trak transport complex.
Epithelial wounding causes waves of actomyosin flow and apical cell constriction that are dependent on calcium signaling and actin filament severing.
Ablation of astrocytic laminin impairs vascular smooth muscle cell function and leads to hemorrhagic stroke
Ablation of astrocytic laminin disrupted the interaction between vascular smooth muscle cells and astrocytes, down-regulated contractile protein expression, and weakened vascular integrity in deep brain regions, leading to hemorrhage.