On the cover
Telley et al. develop a cell-free assay to determine how nuclei disperse through the syncitium of early Drosophila embryos. Condensed chromosomes (red) align on a metaphase spindle (green) in a droplet of cytoplasm extracted from a preblastoderm embryo. After anaphase, the newly formed nuclei are spatially separated by the migration of the microtubule asters through the cytoplasm. Image courtesy of Ivo A. Telley.
See page 887.
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CtIP-dependent DNA end resection, which was previously thought to be necessary for CHK1 kinase activation and subsequent DNA damage checkpoint induction, is in fact only required for sustained checkpoint signaling.
Loss of both meiosis-specific kleisins in mice reveals conserved functions of the meiotic cohesin complexes in axial element formation during spermatogenesis.
Aster migration determines the length scale of nuclear separation in the Drosophila syncytial embryo
A cell-free system using cytoplasmic extract from single–Drosophila syncytial embryos reveals that the spacing between nuclei is preprogrammed and relies on microtubule aster migration.
Use of transgenic mouse eggs expressing human zona pellucida proteins identifies an N-terminal domain of ZP2 that is essential for human sperm–egg binding.
Kinase-inhibited FAK limits VCAM-1 production via nuclear localization and promotion of GATA4 turnover.
Nuclear envelope morphology constrains diffusion and promotes asymmetric protein segregation in closed mitosis
Diffusion barriers and the dumbbell shape of the anaphase nucleus promote nuclear compartmentalization and asymmetric protein inheritance during closed mitosis in yeast.
Arp2/3 complex–dependent actin networks constrain myosin II function in driving retrograde actin flow
Actin veil networks assembled by the Arp2/3 complex constrain myosin II–dependent contractility in neuronal growth cones with consequent effects on peripheral retrograde actin flow rates.
The two head domains of the budding yeast Kinesin-14 Kar3Vik1 bind adjacent protofilaments at the start of the motility cycle, followed by release of Vik1 from one protofilament to allow the motor’s powerstroke.
The dynein-associated protein Lis1 may be a ubiquitous determinant of dynein-dependent transport required primarily at the stage of motility initiation.
Rab5c promotes AMAP1–PRKD2 complex formation to enhance β1 integrin recycling in EGF-induced cancer invasion
EGF signaling activates Rab5c and promotes the intracellular association of AMAP1 and PRKD2 to enhance β1 integrin recycling and promote the invasiveness of breast cancer cells.
Follistatin-mediated skeletal muscle hypertrophy is regulated by Smad3 and mTOR independently of myostatin
Smad3/Akt/mTOR/S6K/S6RP signaling plays a critical role in follistatin-mediated muscle growth that operates independently of myostatin-driven mechanisms.
Sarcospan signals through Akt to increase cell surface levels of utrophin and glycosylated α-dystroglycan and promote muscle repair after injury.