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Cover Image
Cover Image
On the cover
Longatti et al. reveal that recycling endosomes contribute to the formation of autophagosomes in starving cells undergoing autophagy. Overexpression of the Rab GTPase-activating protein TBC1D14 (green) inhibits autophagosome biogenesis by tubulating recycling endosomes containing transferrin (blue) and the autophagy protein Atg13 (red).
Image courtesy of Christopher Lamb.
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In This Issue
In Focus
Nck by the numbers
Meshing experiments and simulations, study uncovers the stoichiometry of actin-polymerizing proteins.
People & Ideas
Graça Raposo: Melanosomes, more than skin deep
Raposo studies the biogenesis of melanosomes as exemplars of lysosome-related organelles.
Review
Report
The C-terminal tail domain of metavinculin, vinculin’s splice variant, severs actin filaments
Relative to vinculin, a unique 68-residue insert in the C-terminal tail of metavinculin results in a loss of actin filament-bundling activity but gain of actin filament-severing activity.
Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes
Mgr2 is a new component of the mitochondrial presequence translocase required for efficient coupling of the TIM23 core complex to Tim21, respiratory chain complexes, and the translocase of the outer mitochondrial membrane.
Talin activates integrins by altering the topology of the β transmembrane domain
Talin binding to the integrin β tail alters the β transmembrane domain’s topology, resulting in integrin activation.
Article
Nucleostemin prevents telomere damage by promoting PML-IV recruitment to SUMOylated TRF1
A novel telomere-protection mechanism relies upon nucleostemin-mediated recruitment of PML-IV to telomeres and subsequent recruitment of RAD51 in both ALT and telomerase-active cells.
LATS1/WARTS phosphorylates MYPT1 to counteract PLK1 and regulate mammalian mitotic progression
Showing convergence with budding yeast mitotic exit network signaling, the LATS1/WARTS kinase phosphorylates the MYPT1 phosphatase to control PLK1 at the G2 DNA damage checkpoint.
Stoichiometry of Nck-dependent actin polymerization in living cells
A combination of computational modeling and quantitative experimentation demonstrates that actin polymerization depends on Nck density as a result of a 4:2:1 Nck/N-WASp/Arp2/3 stoichiometry.
TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes
The noncatalytic RabGAP protein TBC1D14 regulates the Rab11- and ULK1-positive recycling endosomes required for autophagosome formation upon starvation
Basement membrane assembly of the integrin α8β1 ligand nephronectin requires Fraser syndrome–associated proteins
QBRICK facilitates the integrin α8β1–dependent interactions of cells with basement membranes by regulating the basement membrane assembly of nephronectin.
