Continuously dividing cells must be protected from telomeric and nontelomeric DNA damage in order to maintain their proliferative potential. Here, we report a novel telomere-protecting mechanism regulated by nucleostemin (NS). NS depletion increased the number of telomere damage foci in both telomerase-active (TA+) and alternative lengthening of telomere (ALT) cells and decreased the percentage of damaged telomeres associated with ALT-associated PML bodies (APB) and the number of APB in ALT cells. Mechanistically, NS could promote the recruitment of PML-IV to SUMOylated TRF1 in TA+ and ALT cells. This event was stimulated by DNA damage. Supporting the importance of NS and PML-IV in telomere protection, we demonstrate that loss of NS or PML-IV increased the frequency of telomere damage and aberration, reduced telomeric length, and perturbed the TRF2ΔBΔM-induced telomeric recruitment of RAD51. Conversely, overexpression of either NS or PML-IV protected ALT and TA+ cells from telomere damage. This work reveals a novel mechanism in telomere protection.

Introduction

Telomeres protect chromosomal ends from replicative attrition and consist of tandem repeats of telomeric DNAs and a multiprotein complex (Greider and Blackburn, 1985, 1996; de Lange, 2005; Songyang and Liu, 2006). The telomere length plays a crucial role in preserving its integrity, and is maintained by the telomerase in more than 80% of the human cancers (Greider and Blackburn, 1989; Shay et al., 2001). In the other 10–15% of human cancers, the telomerase activity is undetectable (Shay and Bacchetti, 1997). Those telomerase-inactive cells are known as alternative lengthening of telomeres (ALT) cells and are thought to use the homologous recombination (HR) mechanism for telomere maintenance (Bryan et al., 1995, 1997; Shay and Bacchetti, 1997; Liu et al., 2007). One unique feature of ALT cells is the formation of the ALT-associated PML body (APB; Yeager et al., 1999; Dunham et al., 2000), which requires the SUMOylation of TRF1 and TRF2 (Potts and Yu, 2007) and several PML-associated proteins, including PML, MRN complex, RAD52, and RPA (Wu et al., 2000; Zhu et al., 2000; Jiang et al., 2007). The biological role of APB remains unclear, but may be linked to the HR event (Grobelny et al., 2000).

A potential molecule that regulates the telomere integrity in cancer and stem cells is nucleostemin (NS). NS is a nucleolar GTP-binding protein preferentially expressed by multiple types of stem cells and human cancers (Tsai and McKay, 2002; Baddoo et al., 2003; Liu et al., 2004; Ohmura et al., 2008; Nomura et al., 2009; Lin et al., 2010). Its function is required for self-renewal maintenance and early embryogenesis (Tsai and McKay, 2002; Liu et al., 2004; Beekman et al., 2006; Zhu et al., 2006). We previously found that NS and its vertebrate paralogue, guanine nucleotide-binding protein-like 3-like (GNL3L), interact with one of the telomeric proteins, telomeric repeat-binding factor 1 (TRF1; Zhu et al., 2006, 2009; Tsai, 2009), which serves several key functions, including chromosomal end protection (Martínez et al., 2009), telomere shortening (van Steensel and de Lange, 1997), mitotic progression (Zhou et al., 2003, 2009), and APB formation (Potts and Yu, 2005; Jiang et al., 2007). Here, we report a novel mechanism by which NS prevents TIF (telomere dysfunction-induced foci) formation and telomere aberration in both ALT and telomerase-active (TA+) cells. NS does so by promoting the association between PML-IV and SUMOylated TRF1, which increases the telomeric recruitment of RAD51 proteins. We propose that continuously dividing cells may use NS as a protective mechanism to maintain their telomere integrity.

Results

Loss of NS triggers telomeric and nontelomeric DNA damage

We first investigated the effect of NS depletion on the number of telomeric (TIF) and nontelomeric (IDF, interstitial damage foci) damage foci in ALT (U2OS) and TA+ (HeLa) cells. TIF (53BP1+TRF2+) and IDF (53BP1+TRF2) were determined by 3D-reconstructed confocal analyses. Depletion of NS was achieved by the siRNA-mediated knockdown approach, which allowed a significant reduction of NS proteins in U2OS and HeLa cells (90% or more; Fig. S1, A and B). We found that knockdown of NS (NS-KD) by siNS induces a significant increase of TIF in both U2OS and HeLa cells (Fig. 1, A and B). NS-KD also increases the number of damage foci on interstitial chromosomes (IDF) in both U2OS and HeLa cells. The increase of IDF by NS-KD (2.6-fold and 3.5-fold) is less than that of TIF (4.7-fold and 4.2-fold) in either cell type.

NS depletion decreases the percentage of TIF associated with APB and the formation of APB

To investigate the mechanism by which NS depletion triggers telomere damage, we chose to study its effect on APB formation, as APB was linked to the HR event (Grobelny et al., 2000) and NS was shown to interact with an essential component of APB, TRF1 (Zhu et al., 2006). First, we used triple-labeled confocal analyses to determine whether APBs are associated with TIF induced by TRF2ΔBΔM transfection in U2OS cells. TRF2ΔBΔM is a TRF2 mutant that triggers telomere damage by destabilizing the telomeric complex (van Steensel et al., 1998). We found that TRF2ΔBΔM dramatically increases the percentage of APB-occupied TIF (PML+53BP1+TRF1-gfp+ foci divided by 53BP1+TRF1-gfp+ foci) from 0.5% to 35.8% (P < 0.0001), but has no effect on the percentage of APB-occupied undamaged telomeres (10.7% vs. 12.3%; P = 0.28; Fig. 1 C). Importantly, knockdown of NS reduces the TRF2ΔBΔM-triggered increase of APB-occupied TIF from 35.8% to 23.7% (P < 0.05). These results support a link between APB and telomere damage in U2OS cells and suggest that NS may have a role in the formation of APB. Indeed, NS-KD significantly reduces the percentage of APB (PML+TRF2+) among PML bodies from 60.3% to 30.4% (P < 0.0001; Fig. 1 D), and NS overexpression shows the opposite effect (Fig. 1, E3). To determine whether the nucleolar localization of NS is required for its APB-regulatory function, we measured the effect of a nucleoplasmic mutant of NS, NSdB, which is deleted of its nucleolar localization signal (NoLS, aa 1–46) at the N terminus (Meng et al., 2006). NSdB is distributed predominantly in the nucleoplasm (Fig. 1, E1), but still retains the ability to bind TRF1 (Fig. 1, E2). Compared with the wild-type NS, NSdB shows a similar but stronger effect in promoting the formation of APB in U2OS cells (Fig. 1, E3), indicating that the telomere function of NS takes place outside the nucleolus. These results suggest that NS may prevent telomere damage by stimulating the formation of APB in ALT cells. Because NS perturbation does not change the number of PML bodies, TRF2 foci, or TRF1-gfp foci (Fig. S1, C and D), we reason that NS may increase APB by promoting the association between telomeres and PML bodies.

NS promotes PML body recruitment to SUMOylated TRF1

Because APB formation requires SUMOylation on TRF1 (Potts and Yu, 2007), we next determined whether NS regulates TRF1 SUMOylation. Our results showed that NS overexpression increases TRF1 SUMOylation and NS knockdown does the opposite (Fig. 2, A and B). Interestingly, GNL3L perturbation shows the opposite effects on TRF1 SUMOylation compared with NS perturbation. Because TRF1 SUMOylation is catalyzed by an E3 ligase, MMS21 (Potts and Yu, 2007), we examined how NS and GNL3L affect the interaction between TRF1 and MMS21. Coimmunoprecipitation (coIP) showed that the binding between TRF1 and MMS21 is reduced by GNL3L overexpression but not affected by NS overexpression (Fig. 2 C). As NS and GNL3L display opposite effects on APB formation and TRF1 SUMOylation, we tested whether GNL3L depletion causes DNA damage, and found that GNL3L knockdown has no effect on the formation of TIF or IDF in either ALT or TA+ cells (Fig. S2, A and B). These findings suggest that TRF1 SUMOylation by itself may not be a sufficient determinant to explain the TIF-regulatory activity of NS.

To explore the possibility that NS may regulate APB formation after the TRF1 SUMOylation step, we designed a C-terminally SUMO1-fused TRF1 construct (TRF1-So) to mimic the mono-SUMOylated TRF1 protein (Fig. 3 A, left). This design is based on a similar strategy used to study p53 monoubiquitination (Carter et al., 2007). The six C-terminal residues on the SUMO1 fusion of TRF1-So, including the di-Gly motif, were deleted to avoid direct conjugation of TRF1-So to off-target proteins. A SUMO-compromised mutant (TRF1R6) was also created by mutating the six SUMO-targeting Lys residues to Arg, as described in Potts and Yu (2007). TRF1-So and TRF1R6 display a higher (51.9%) and a lower (29.0%) percentage of APB among PML bodies (APB/PML) than does TRF1 (39.6%; P < 0.001), respectively, and may therefore mimic the SUMOylated and deSUMOylated states of TRF1 (Fig. 3 A, right). The APB/PML percentage of a compound mutant, TRF1R6-So, resembles that of TRF1-So, suggesting that the SUMO conjugate acts dominantly over the R6 mutant. We first confirmed that all mutant proteins colocalize with the endogenous TRF2 signal at the telomere (Fig. S3). NS knockdown reduces the APB/PML percentage of TRF1-So from 50.9 to 42.7% (P < 0.01), and NS overexpression does the opposite (67.9%; P < 0.001; Fig. 3 B). In contrast, GNL3L perturbation has no effect on the APB/PML percentage of TRF1-So. These results indicate that the APB- and TIF-regulatory activities of NS regulate the recruitment of PML bodies to SUMOylated TRF1, whereas the APB activity of GNL3L targets the SUMOylation event of TRF1.

NS promotes the interaction between SUMOylated TRF1 and PML-IV

To determine the molecular event mediating the association between PML bodies and SUMOylated TRF1, we performed coIP of TRF1-So and different PML isoforms (Jensen et al., 2001). We found that TRF1-So interacts strongly with PML-IV (X63131) but not with PML-III (S50913) or PML-VI (M80185; Fig. 3 C, left). TRF1 itself shows a detectable but much weaker binding to PML-IV compared with TRF1-So (Fig. 3 C, right; and Fig. S4 A). A PML-IV-specific siRNA was synthesized that can knock down more than 60% of the PML-IV transcript without affecting PML-I, which shares the most sequence identity with PML-IV (Fig. 3 D). Supporting the role of PML-IV in APB regulation, PML-IV knockdown decreases the APB/PML percentage from 60.3 to 46.1% (P < 0.001) without changing the total number of PML bodies per cell (P = 0.60; Fig. 3 E). In addition, NS overexpression significantly increases the binding between TRF1-So and PML-IV (Fig. 3 F, left), and NS knockdown does the opposite (Fig. 3 F, right). For control experiments, we confirmed that NS interacts with TRF1 and TRF1-So equally well (Fig. S4 B) but not with PML-IV (Fig. S4 C), and excluded the possibility that NS and PML-IV bind TRF1-So through its C-terminal SUMO-1 fusion (Fig. S4 D). Furthermore, the NS ability to promote the association between SUMOylated TRF1 (TRF1-So) and PML bodies is completely abolished by knockdown of PML-IV (P < 0.001; Fig. 3 G). As APB is a hallmark of ALT cells, it is unclear whether this NS-regulated recruitment of PML bodies to the telomere occurs in TA+ cells. To address this issue, we examined the formation of APB in HeLa cells (Fig. 3 H). HeLa cells contain few APBs under normal conditions (7.9% of total PML), but show a significant increase of APBs under the TRF2ΔBΔM-induced telomere damage condition (34.6%; P < 0.0001). Notably, knockdown of NS or PML-IV reduces the TRF2ΔBΔM-triggered APB formation to 23.8 and 19.5%, respectively. These results demonstrate that NS promotes the telomeric recruitment of PML bodies by increasing the association between SUMOylated TRF1 and PML-IV in both ALT and TA+ cells.

DNA damage increases the interaction between SUMOylated TRF1 and PML-IV in an NS-dependent manner

Because TA+ cells show APB formation only under the TRF2ΔBΔM-transfected condition, we asked whether DNA damage increases the recruitment of PML bodies to SUMOylated TRF1 foci in an NS-dependent manner in U2OS cells. DNA damage was induced by etoposide, which is a topoisomerase II inhibitor capable of inducing telomeric and nontelomeric DNA damage (Yoon et al., 1998; Zhang et al., 2003; Kang et al., 2004). We showed that etoposide can significantly increase the APB/PML percentage of TRF1-So from 51.9 to 83.6% (P < 0.001), and that NS-KD abolishes most of this effect (61.2%; Fig. 4 A). CoIP showed that both TRF2ΔBΔM-induced telomere damage and etoposide-induced DNA damage increase TRF1-So binding to PML-IV (Fig. 4 B), and that etoposide treatment increases the interaction between NS and TRF1-So (Fig. 4 C). Importantly, the increased binding between PML-IV and TRF1-So after etoposide treatment is abrogated by NS-KD (Fig. 4 D). To determine the interaction between native NS and TRF1 proteins under normal and DNA damage conditions, we performed endogenous coIP experiments in HeLa cells. The results confirmed that the endogenous NS and TRF1 proteins bind each other under the undamaged condition and that their interaction is increased by etoposide treatment (Fig. 4 E). Similarly, binding between endogenous TRF1 and PML proteins is significantly increased under the DNA damage condition (Fig. 4 F). More importantly, a high molecular weight product of TRF1 (∼73 kD) is co-purified with PML proteins under the DNA damage condition. This same product is recognized by the anti–SUMO-1 antibody and hence represents the SUMOylated form of TRF1. The ratio of SUMOylated-to-non-SUMOylated TRF1 proteins is significantly greater in the pull-down fraction than in the input lysate, indicating that PML binding enriches for the SUMOylated form of TRF1. These results support that DNA damage increases the interaction between SUMOylated TRF1 and PML proteins.

Loss of PML-IV or NS increases the frequency of telomere damage and aberration

To demonstrate the importance of PML-IV in reducing telomere damage, we showed that loss of PML-IV in U2OS cells increases the number of TIF per cell by threefold (from 0.17 to 0.50; P < 0.01) and IDF by 2.4-fold (from 1.2 to 2.9; P < 0.01; Fig. 5 A). In HeLa cells, PML-IV depletion also increases the number of TIF (from 0.17 to 0.54; P < 0.01) and IDF (from 0.49 to 1.24; P < 0.001; Fig. 5 B). To establish the biological significance of NS and PML-IV in maintaining the telomere integrity, we measured their knockdown effects on telomere abnormalities in U2OS cells by telomere fluorescence in situ hybridization (Tel-FISH). Tel-FISH showed that loss of NS increases the frequency of chromosomes with low telomere signals (from 27.2 to 42.3%; P < 0.0001) and the frequency of chromosomal fusion (from 3.1 to 5.9%; P < 0.001; Fig. 5 C). More than 90% of the increased chromosomal fusion occurs between sister chromatids. Similarly, PML-IV depletion increases the frequency of chromosomes with low telomere signals (44.5%; P < 0.0001) but has no effect on the chromosomal fusion event. Low telomere signals indicate that the telomere length is decreased. Quantitative FISH (Q-FISH) analyses showed that the average telomere intensities of NS-KD and PML-KD U2OS cells are significantly lower than that of control cells (Fig. 5 D). These data demonstrate the effect of PML-IV knockdown in causing telomeric and nontelomeric DNA damage and the roles of NS and PML-IV in maintaining the telomere integrity.

NS and PML-IV promotes the telomeric recruitment of RAD51 induced by telomere damage

RAD51 is the core protein in the HR-based repair of damaged telomeres (Kibe et al., 2003; Carneiro et al., 2010). To determine whether NS or PML-IV regulates the recruitment of RAD51 to the telomere under the telomere damage condition, U2OS cells were transfected with TRF2ΔBΔM and measured for the colocalized RAD51 and TRF1-GFP signals. The results showed that the number of telomere-associated RAD51 foci is greatly increased by TRF2ΔBΔM-induced telomere damage (from 0.7 to 2.7 per cell; P < 0.0001), and that knockdown of NS or PML-IV reduces the frequency of this event (1.7 and 1.5 per cell; P < 0.01; Fig. 6 A, left). Conversely, overexpression of NS or PML-IV under the telomere damage condition increases the telomere recruitment of RAD51 from 2.7 to 3.4 (P < 0.001) and 6.0 per cell (P < 0.0001), respectively (Fig. 6 B, left). As NS knockdown increases nontelomeric RAD51 foci (P < 0.001; Fig. 6 A, right) and NS overexpression reduces them (P < 0.0001; Fig. 6 B, right), we reason that the NS function is to promote the telomeric recruitment of RAD51. On the other hand, overexpression of PML-IV increases telomeric RAD51 foci without reducing the number of nontelomeric RAD51 foci (Fig. 6 B), which suggests that overexpression of PML-IV may increase the formation of RAD51 foci. We confirmed this idea by showing that overexpression of PML-IV alone significantly increases the formation of RAD51 foci without triggering DNA damage, and that all RAD51 foci colocalize with PML-IV signals (Fig. S5), which suggests that PML-IV may directly interact with RAD51.

The interaction between RAD51 and TRF1-So or PML-IV was further investigated by coIP experiments. Regular coIP showed a direct interaction between PML-IV and RAD51 but a very weak binding between TRF1-So and RAD51, with or without PML-IV (unpublished data). The lack of binding between TRF1-So and RAD51 in regular coIP suggests that this event may be transient and highly regulated. Therefore, we used the cross-link coIP approach to delineate the binding relationship between RAD51 and TRF1-So. Cross-link coIP showed that PML-IV is required for the specific interaction between RAD51 (FLAG) and TRF1-So (HA), and that this interaction is increased by TRF2ΔBΔM-induced telomere damage (Fig. 6 C, lanes 1–5). By contrast, PML-IV binds RAD51 directly and independently of telomere damage (Fig. 6 D). These results support that NS promotes the association between RAD51-bound PML-IV and SUMOylated TRF1, and that this mechanism is activated by telomere damage.

NS and PML-IV protect ALT and TA+ cells from telomere damage

To demonstrate the function of NS and PML-IV in protecting against telomere damage, we measured the effect of NS or PML-IV overexpression on the formation of TRF2ΔBΔM-induced TIF. First, we confirmed that TRF2ΔBΔM transfection significantly increases the amount of TIF in both U2OS (Fig. 7 A) and HeLa cells (Fig. 7 B). In TRF2ΔBΔM-transfected U2OS cells, overexpression of NS reduces the number of TIF from 4.3 to 1.8 per cell (P < 0.0001; Fig. 7 A). Overexpression of PML-IV in TRF2ΔBΔM-transfected U2OS cells shows the same effect (TIF = 1.9 per cell; P < 0.0001). In TRF2ΔBΔM-transfected HeLa cells, overexpression of NS or PML-IV decreases the number of TIF from 2.1 to 0.9 and 0.7 per cell, respectively (P < 0.001; Fig. 7 B). Notably, the nucleoplasmic mutant of NS, NSdB, is also capable of protecting against TRF2ΔBΔM-induced telomere damage in U2OS and HeLa cells (Fig. 7 C). This activity of NSdB is even stronger than that of wild-type NS in U2OS cells. To determine whether PML-IV is also involved in the nontelomeric DNA damage induced by NS-KD, we used PML-IV to rescue the DNA damage phenotype of NS-KD cells (Fig. 7 D). Our results showed that PML-IV is able to partially rescue the telomeric and nontelomeric DNA damage induced by NS-KD in both TA+ and ALT cells. These findings indicate that the NS-regulated TRF1–PML-IV pathway protects both TA+ and ALT cells from telomere damage.

Discussion

NS protects ALT and TA+ cells from telomere damage

In this study, we report the NS function in maintaining the integrity of telomeric and interstitial chromosomes and determine the mechanism underlying the telomere-protecting activity of NS. Even though loss of NS triggers DNA damage on both telomeric and interstitial chromosomes, the NS-KD effect is more notable on the telomere than on the nontelomeric chromosome. The NS role in preventing TIF formation is supported by the findings that NS knockdown triggers spontaneous telomere damage, NS overexpression protects against TRF2ΔBΔM-induced telomere damage, NS depletion reduces the telomere length, and loss of NS increases the frequency of chromosomes with low telomere signals and sister chromatid fusion. Because NS is most abundantly expressed in cancer and stem cells, such a mechanism may allow these cells to quickly respond to and repair telomere damage in order to maintain their self-renewing proliferation. Telomere elongation in embryonic and adult stem cells is normally attributed to the telomerase activity, which alone may not be sufficient over time. Our discovery suggests the possibility that self-renewing cells may be equipped with the NS machinery to safeguard their telomere integrity during the aging process.

NS promotes the association of SUMOylated TRF1 and PML-IV

Our mechanistic investigation on the TIF-regulatory activity of NS was based on its ability to bind TRF1 and increase APB formation. APB is a known feature of ALT cells. It was postulated that APB may serve the function of sequestering low molecular weight telomeric DNAs, as APB contains linear extrachromosomal telomere repeat DNA (Fasching et al., 2007). Others suggested that APB may be linked to the HR function based on its content of DNA damage response and repair proteins and its DNA synthesis activity (Yeager et al., 1999; Wu et al., 2000, 2003; Nabetani et al., 2004). We found that TRF2ΔBΔM-induced telomere damage increases the percentage of APB-occupied TIF but not the percentage of APB-occupied undamaged telomeres, and that NS knockdown reduces the percentage of APB-occupied TIF. These findings suggest a link between APB and telomere damage and a role of NS in APB formation. Indeed, NS can increase APB by promoting the association between telomeres and PML bodies. To define the molecular interaction between telomeres and PML bodies, we discovered a specific binding between PML-IV and SUMOylated TRF1, which can be increased by NS and DNA damage signals. Because PML-IV interacts much more strongly with SUMO-modified TRF1 than with wild-type TRF1 and does not bind NS directly, we propose that NS binding and SUMOylation of TRF1 may trigger a conformational change that favors its association with PML-IV, which then brings PML-IV–tethered proteins (e.g., RAD51) to the telomere under damage conditions (Fig. 7 E). The activity of NS to promote APB formation in U2OS cells or to protect against TRF2ΔBΔM-induced telomere damage in U2OS or HeLa cells does not require its nucleolar localization. In fact, the NSdB mutant performs even more efficiently than the wild-type NS in promoting APB or reducing TIF in U2OS cells, indicating that the telomere function of NS takes place outside the nucleolus. In conjunction with our previous results showing that the NS protein is not recruited to the telomere (Meng et al., 2011), we propose that the NS-mediated regulation on the interaction between TRF1 and PML occurs in the nucleoplasm and extra-chromosomally before they are incorporated into stable large protein complexes on the telomere.

The role of PML-IV in mediating the telomere-protecting function of NS is supported by the knockdown experiments, which show that loss of PML-IV produces the same TIF phenotypes as NS-KD does, and that overexpression of PML-IV or NS can protect against TRF2ΔBΔM-induced telomere damage. In addition, PML-IV is required for the NS-induced association of PML bodies and SUMOylated TRF1 foci, as well as the telomeric recruitment of RAD51 under the telomere damage condition. More importantly, overexpression of PML-IV is able to rescue the telomere damage phenotype of NS-KD in both TA+ and ALT cells. Whereas loss of NS or PML-IV both show increased numbers of chromosomes with low telomere signals, only NS-KD increases the frequency of chromosomal fusion, which raises the possibility that PML-IV may not be the sole mediator for this NS activity. Based on the lack of TRF1 binding by PML-VI and a previous finding that narrows down the TRF1-interactive domain of PML-IV to its C-terminal 154 residues (Yu et al., 2010), one may deduce that TRF1 binding involves the C-terminal 81 residues of PML-IV. Because 84% of this region is found in PML-I, it is possible that PML-I may also interact with SUMOylated TRF1 and play a redundant or complementary role as compared with PML-IV.

The ALT-related activity of NS in TA+ cells and on interstitial chromosomes

As NS knockdown triggers spontaneous DNA damage not only on the telomere of ALT cells but also on the telomere of TA+ cells and on the interstitial chromosome of both TA+ and ALT cells, it raises the question of whether the proposed mechanism of NS (i.e., the telomeric recruitment of PML-IV) works in TA+ cells or on nontelomeric chromosomes. Two lines of evidence support that this mechanism is also in use in TA+ cells. First, in both ALT and TA+ cells, loss of PML-IV induces telomere damage and overexpression of PML-IV rescues the telomere damage effect of NS-KD. Second, APB can be induced by TRF2ΔBΔM-triggered telomere damage in HeLa (TA+) cells, and knockdown of NS or PML-IV significantly decreases the frequency of this event. On the issue of telomeric versus nontelomeric damage, the knockdown and rescue results, too, support the involvement of PML-IV in alleviating nontelomeric DNA damage.

NS and GNL3L differentially regulate APB and TIF

Our data demonstrate that NS and GNL3L exert opposite effects on TRF1 SUMOylation. Given that NS and GNL3L compete against each other for TRF1 binding (Meng et al., 2011), this TRF1 SUMOylation effect of NS may be explained by its ability to displace TRF1-bound GNL3L. On the other hand, only NS but not GNL3L can regulate the association between PML and SUMOylated TRF1. It is noted that even though GNL3L shows the opposite effect on APB regulation (Zhu et al., 2009) and TRF1 SUMOylation compared with NS, it does not exhibit the activity to regulate TIF formation as does NS. Therefore, TRF1 SUMOylation alone may not be sufficient to explain the TIF-regulatory function of NS. Furthermore, the differential activities of NS and GNL3L in APB and TIF regulation also suggest that APB may be heterogeneous. Not all of them are connected to telomere repair. Evolutionarily, NS and GNL3L share the same invertebrate orthologue, GNL3 (Tsai and Meng, 2009). The functional divergence of NS and GNL3L may signify an expansion in the telomere-regulatory modality during vertebrate evolution. Whereas NS extends the proliferative lifespan by providing damaged telomeres a better access to repair proteins, GNL3L may serve the role of stabilizing the telomere structure in differentiated cells.

Materials and methods

cDNA constructs and antibodies

Point mutation and fusion of TRF1 were introduced by the stitching PCR strategy (Tsai and McKay, 2005). TRF2ΔBΔM includes amino acids 45–454. Epitope-tagged expression constructs of PML isoforms were made by cloning cDNA fragments PCR amplified from the original PML constructs into the Myc- or FLAG-tagged vector. Primary antibodies include anti-HA (HA.11; Covance), Myc (9E10; Covance), FLAG (M2; Sigma-Aldrich), TRF1 (TRF-78; Santa Cruz Biotechnology, Inc.), TRF2 (4A794; EMD), PML (PG-M3, H-238; Santa Cruz Biotechnology, Inc.), 53BP1 (4937; Cell Signaling Technology), RAD51 (51RAD01; Thermo Fisher Scientific), SUMO-1 (FL-101; Santa Cruz Biotechnology, Inc.), and NS (Ab2438 and Ab138). Secondary antibodies are conjugated to Rhodamine-X, FITC, or peroxidase.

siRNA knockdown

Knockdown was done by transfecting cells with shRNAmir constructs or siRNA duplexes using the Lipofectamine Plus or Oligofectamine reagent, respectively. The sequences targeted by the control (siScr), NS-specific (siNS), GNL3L-specific (siG3L), and siPML-IV-specific (siPML4) siRNA duplexes, as well as the control (shScr), NS-specific (shNS), and GNL3L-specific (shG3L) shRNAmir are as follows. siScr, 5′-TGACGATCAGAATGCGACT-3′; siNS, 5′-GAACTAAAACAGCAGCAGA-3′; siG3L, 5′-AAACGCAGGACCATTGAGA-3′; siPML4, 5′-TGACAATGAAAGTGGGTTC-3′; shScr, 5′-TCTCGCTTGGGCGAGAGTAAG-3′; shNS, 5′-CCTGATATTAAGCCATCAAAT-3′; shG3L, 5′-CCAATCGAGAGGCTGAATTAA-3′.

Immunofluorescence and confocal quantification of TIF, APB, and RAD51 foci

TIF were determined by the colocalization of 53BP1 and TRF2 (or TRF1-GFP) signals. The number of APBs was scored on the basis of colocalized PML and TRF2 (TRF1-GFP, or FLAG-tagged TRF1) signals. To detect RAD51 foci, cells were incubated with the permeabilization solution (10 mM Hepes, 100 mM NaCl, 3 mM MgCl2, 300 mM sucrose, and 0.5% Triton) for 2 min on ice before the fixation step. Images were acquired on a confocal microscope (LSM510; Carl Zeiss) using a 63x Plan Apochromat oil objective (1.4 NA) at room temperature. Scanning was set with a 512 x 512 frame size, 3x zoom, and <1.0-µm optical thickness. Stacked images of 80–100 randomly chosen cells were collected at 0.5-µm intervals from five independent experiments and analyzed using ImageJ 1.36b software (National Institutes of Health, Bethesda, MD).

In vivo SUMOylation assay

His-tagged SUMO-1 and TRF1 expression plasmids were coexpressed in HEK293 cells. 2 d later, protein lysates were extracted in 6M guanidinium buffer, pulled down by Ni2+-chelating Sepharose in 3 h, and washed carefully to prevent degradation of SUMOylated products.

Coimmunoprecipitation

For regular coIP experiments, protein lysates were extracted in NTEN buffer without cross-linking. For coIP of TRF1-So and RAD51, protein complexes were cross-linked with 1% formaldehyde in PBS for 20 min at 4°C and incubated with 125 mM glycine in PBS before the extraction step. Protein extracts were precleared by microcentrifugation and incubated with primary antibody and protein G–Sepharose (GE Healthcare) for 4 h at 4°C. Immunoprecipitates were washed extensively before SDS-PAGE. For endogenous coIP experiments, the NS and PML protein complexes were immunoprecipitated in HeLa cells by anti-NS (Ab138) or anti-PML (PG-M3) antibody, respectively. Precipitated NS and PML proteins were immunodetected by the Ab2438 or H-238 antibody, respectively.

Telomere fluorescence in situ hybridization (T-FISH) and quantitative-FISH (Q-FISH)

Cells were treated with colcemid (0.1 µg/ml) for 16 h, incubated in hypotonic solution (0.8% sodium citrate), and fixed in methanol/acetic acid (3:1) before spreading onto slides. The T-FISH procedure was conducted by incubating metaphase-spread chromosomes with a Cy3-conjugated telomere-specific peptide nucleic acid (PNA) probe, (C3TA2)2, in hybridization buffer (70% formamide, 10 mM Tris, pH 7.2) for 3 h at 25°C and counterstaining the chromosomes with TO-PRO-3 dyes. High-resolution stacked images were taken on the LSM510 confocal imaging platform (Carl Zeiss) using the 63x Plan Apochromat oil objective, and quantified for the frequency of telomere aberration on each metaphase-spread chromosome. The relative telomere length was measured by Q-FISH, where individual telomere signal intensities of T-FISH were scored using the ImageJ telomeric plug-in program.

Online supplemental material

Fig. S1 shows experiments for the siNS-mediated knockdown efficiency and the effect of NS perturbation on TRF2 foci and PML bodies. Fig. S2 shows that loss of GNL3L does not predispose ALT or TA+ cells to telomere or DNA damage. Fig. S3 shows that GFP- and FLAG-tagged TRF1 proteins colocalize with the endogenous TRF2. Fig. S4 shows control experiments for the binding specificity between TRF1, PML-IV, NS, and/or GNL3L. Fig. S5 shows that overexpression of PML-IV promotes Rad51 foci formation without increasing DNA damage.

Acknowledgments

Our gratitude goes to the following investigators for their generous gifts of cDNA constructs: Dr. Zhou Songyang (Baylor College of Medicine, Houston, TX) for TRF2ΔBΔM; Dr. Hongtao Yu (UT Southwestern, Dallas, TX) for MMS21; Dr. Kun-Sang Chang (MD Anderson Cancer Center, Houston, TX) for PML-III and PML-IV; Dr. Edward Yeh (MD Anderson Cancer Center) for Rad51. We also thank Lingjun Meng for his help on western blot and Hsi-Wen Tsai for his unwavering support.

References

References
Baddoo
M.
,
Hill
K.
,
Wilkinson
R.
,
Gaupp
D.
,
Hughes
C.
,
Kopen
G.C.
,
Phinney
D.G.
.
2003
.
Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection
.
J. Cell. Biochem.
89
:
1235
1249
.
Beekman
C.
,
Nichane
M.
,
De Clercq
S.
,
Maetens
M.
,
Floss
T.
,
Wurst
W.
,
Bellefroid
E.
,
Marine
J.C.
.
2006
.
Evolutionarily conserved role of nucleostemin: controlling proliferation of stem/progenitor cells during early vertebrate development
.
Mol. Cell. Biol.
26
:
9291
9301
.
Bryan
T.M.
,
Englezou
A.
,
Gupta
J.
,
Bacchetti
S.
,
Reddel
R.R.
.
1995
.
Telomere elongation in immortal human cells without detectable telomerase activity
.
EMBO J.
14
:
4240
4248
.
Bryan
T.M.
,
Englezou
A.
,
Dalla-Pozza
L.
,
Dunham
M.A.
,
Reddel
R.R.
.
1997
.
Evidence for an alternative mechanism for maintaining telomere length in human tumors and tumor-derived cell lines
.
Nat. Med.
3
:
1271
1274
.
Carneiro
T.
,
Khair
L.
,
Reis
C.C.
,
Borges
V.
,
Moser
B.A.
,
Nakamura
T.M.
,
Ferreira
M.G.
.
2010
.
Telomeres avoid end detection by severing the checkpoint signal transduction pathway
.
Nature.
467
:
228
232
.
Carter
S.
,
Bischof
O.
,
Dejean
A.
,
Vousden
K.H.
.
2007
.
C-terminal modifications regulate MDM2 dissociation and nuclear export of p53
.
Nat. Cell Biol.
9
:
428
435
.
de Lange
T.
2005
.
Shelterin: the protein complex that shapes and safeguards human telomeres
.
Genes Dev.
19
:
2100
2110
.
Dunham
M.A.
,
Neumann
A.A.
,
Fasching
C.L.
,
Reddel
R.R.
.
2000
.
Telomere maintenance by recombination in human cells
.
Nat. Genet.
26
:
447
450
.
Fasching
C.L.
,
Neumann
A.A.
,
Muntoni
A.
,
Yeager
T.R.
,
Reddel
R.R.
.
2007
.
DNA damage induces alternative lengthening of telomeres (ALT) associated promyelocytic leukemia bodies that preferentially associate with linear telomeric DNA
.
Cancer Res.
67
:
7072
7077
.
Greider
C.W.
,
Blackburn
E.H.
.
1985
.
Identification of a specific telomere terminal transferase activity in Tetrahymena extracts
.
Cell.
43
:
405
413
.
Greider
C.W.
,
Blackburn
E.H.
.
1989
.
A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis
.
Nature.
337
:
331
337
.
Greider
C.W.
,
Blackburn
E.H.
.
1996
.
Telomeres, telomerase and cancer
.
Sci. Am.
274
:
92
97
.
Grobelny
J.V.
,
Godwin
A.K.
,
Broccoli
D.
.
2000
.
ALT-associated PML bodies are present in viable cells and are enriched in cells in the G(2)/M phase of the cell cycle
.
J. Cell Sci.
113
:
4577
4585
.
Jensen
K.
,
Shiels
C.
,
Freemont
P.S.
.
2001
.
PML protein isoforms and the RBCC/TRIM motif
.
Oncogene.
20
:
7223
7233
.
Jiang
W.Q.
,
Zhong
Z.H.
,
Henson
J.D.
,
Reddel
R.R.
.
2007
.
Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference
.
Oncogene.
26
:
4635
4647
.
Kang
M.R.
,
Muller
M.T.
,
Chung
I.K.
.
2004
.
Telomeric DNA damage by topoisomerase I. A possible mechanism for cell killing by camptothecin
.
J. Biol. Chem.
279
:
12535
12541
.
Kibe
T.
,
Tomita
K.
,
Matsuura
A.
,
Izawa
D.
,
Kodaira
T.
,
Ushimaru
T.
,
Uritani
M.
,
Ueno
M.
.
2003
.
Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer
.
Nucleic Acids Res.
31
:
5054
5063
.
Lin
T.
,
Meng
L.
,
Li
Y.
,
Tsai
R.Y.
.
2010
.
Tumor-initiating function of nucleostemin-enriched mammary tumor cells
.
Cancer Res.
70
:
9444
9452
.
Liu
S.J.
,
Cai
Z.W.
,
Liu
Y.J.
,
Dong
M.Y.
,
Sun
L.Q.
,
Hu
G.F.
,
Wei
Y.Y.
,
Lao
W.D.
.
2004
.
Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies
.
World J. Gastroenterol.
10
:
1246
1249
.
Liu
L.
,
Bailey
S.M.
,
Okuka
M.
,
Muñoz
P.
,
Li
C.
,
Zhou
L.
,
Wu
C.
,
Czerwiec
E.
,
Sandler
L.
,
Seyfang
A.
et al
.
2007
.
Telomere lengthening early in development
.
Nat. Cell Biol.
9
:
1436
1441
.
Martínez
P.
,
Thanasoula
M.
,
Muñoz
P.
,
Liao
C.
,
Tejera
A.
,
McNees
C.
,
Flores
J.M.
,
Fernández-Capetillo
O.
,
Tarsounas
M.
,
Blasco
M.A.
.
2009
.
Increased telomere fragility and fusions resulting from TRF1 deficiency lead to degenerative pathologies and increased cancer in mice
.
Genes Dev.
23
:
2060
2075
.
Meng
L.
,
Yasumoto
H.
,
Tsai
R.Y.
.
2006
.
Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm
.
J. Cell Sci.
119
:
5124
5136
.
Meng
L.
,
Hsu
J.K.
,
Zhu
Q.
,
Lin
T.
,
Tsai
R.Y.
.
2011
.
Nucleostemin inhibits TRF1 dimerization and shortens its dynamic association with the telomere
.
J. Cell Sci.
124
:
3706
3714
.
Nabetani
A.
,
Yokoyama
O.
,
Ishikawa
F.
.
2004
.
Localization of hRad9, hHus1, hRad1, and hRad17 and caffeine-sensitive DNA replication at the alternative lengthening of telomeres-associated promyelocytic leukemia body
.
J. Biol. Chem.
279
:
25849
25857
.
Nomura
J.
,
Maruyama
M.
,
Katano
M.
,
Kato
H.
,
Zhang
J.
,
Masui
S.
,
Mizuno
Y.
,
Okazaki
Y.
,
Nishimoto
M.
,
Okuda
A.
.
2009
.
Differential requirement for nucleostemin in embryonic stem cell and neural stem cell viability
.
Stem Cells.
27
:
1066
1076
.
Ohmura
M.
,
Naka
K.
,
Hoshii
T.
,
Muraguchi
T.
,
Shugo
H.
,
Tamase
A.
,
Uema
N.
,
Ooshio
T.
,
Arai
F.
,
Takubo
K.
et al
.
2008
.
Identification of stem cells during prepubertal spermatogenesis via monitoring of nucleostemin promoter activity
.
Stem Cells.
26
:
3237
3246
.
Potts
P.R.
,
Yu
H.
.
2005
.
Human MMS21/NSE2 is a SUMO ligase required for DNA repair
.
Mol. Cell. Biol.
25
:
7021
7032
.
Potts
P.R.
,
Yu
H.
.
2007
.
The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins
.
Nat. Struct. Mol. Biol.
14
:
581
590
.
Shay
J.W.
,
Bacchetti
S.
.
1997
.
A survey of telomerase activity in human cancer
.
Eur. J. Cancer.
33
:
787
791
.
Shay
J.W.
,
Zou
Y.
,
Hiyama
E.
,
Wright
W.E.
.
2001
.
Telomerase and cancer
.
Hum. Mol. Genet.
10
:
677
685
.
Songyang
Z.
,
Liu
D.
.
2006
.
Inside the mammalian telomere interactome: regulation and regulatory activities of telomeres
.
Crit. Rev. Eukaryot. Gene Expr.
16
:
103
118
.
Tsai
R.Y.
2009
.
Nucleolar modulation of TRF1: a dynamic way to regulate telomere and cell cycle by nucleostemin and GNL3L
.
Cell Cycle.
8
:
2912
2916
.
Tsai
R.Y.
,
McKay
R.D.
.
2002
.
A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells
.
Genes Dev.
16
:
2991
3003
.
Tsai
R.Y.
,
McKay
R.D.
.
2005
.
A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin
.
J. Cell Biol.
168
:
179
184
.
Tsai
R.Y.
,
Meng
L.
.
2009
.
Nucleostemin: a latecomer with new tricks
.
Int. J. Biochem. Cell Biol.
41
:
2122
2124
.
van Steensel
B.
,
de Lange
T.
.
1997
.
Control of telomere length by the human telomeric protein TRF1
.
Nature.
385
:
740
743
.
van Steensel
B.
,
Smogorzewska
A.
,
de Lange
T.
.
1998
.
TRF2 protects human telomeres from end-to-end fusions
.
Cell.
92
:
401
413
.
Wu
G.
,
Lee
W.H.
,
Chen
P.L.
.
2000
.
NBS1 and TRF1 colocalize at promyelocytic leukemia bodies during late S/G2 phases in immortalized telomerase-negative cells. Implication of NBS1 in alternative lengthening of telomeres
.
J. Biol. Chem.
275
:
30618
30622
.
Wu
G.
,
Jiang
X.
,
Lee
W.H.
,
Chen
P.L.
.
2003
.
Assembly of functional ALT-associated promyelocytic leukemia bodies requires Nijmegen Breakage Syndrome 1
.
Cancer Res.
63
:
2589
2595
.
Yeager
T.R.
,
Neumann
A.A.
,
Englezou
A.
,
Huschtscha
L.I.
,
Noble
J.R.
,
Reddel
R.R.
.
1999
.
Telomerase-negative immortalized human cells contain a novel type of promyelocytic leukemia (PML) body
.
Cancer Res.
59
:
4175
4179
.
Yoon
H.J.
,
Choi
I.Y.
,
Kang
M.R.
,
Kim
S.S.
,
Muller
M.T.
,
Spitzner
J.R.
,
Chung
I.K.
.
1998
.
DNA topoisomerase II cleavage of telomeres in vitro and in vivo
.
Biochim. Biophys. Acta.
1395
:
110
120
.
Yu
J.
,
Lan
J.
,
Wang
C.
,
Wu
Q.
,
Zhu
Y.
,
Lai
X.
,
Sun
J.
,
Jin
C.
,
Huang
H.
.
2010
.
PML3 interacts with TRF1 and is essential for ALT-associated PML bodies assembly in U2OS cells
.
Cancer Lett.
291
:
177
186
.
Zhang
P.
,
Chan
S.L.
,
Fu
W.
,
Mendoza
M.
,
Mattson
M.P.
.
2003
.
TERT suppresses apoptotis at a premitochondrial step by a mechanism requiring reverse transcriptase activity and 14-3-3 protein-binding ability
.
FASEB J.
17
:
767
769
.
Zhou
X.Z.
,
Perrem
K.
,
Lu
K.P.
.
2003
.
Role of Pin2/TRF1 in telomere maintenance and cell cycle control
.
J. Cell. Biochem.
89
:
19
37
.
Zhu
X.D.
,
Küster
B.
,
Mann
M.
,
Petrini
J.H.
,
de Lange
T.
.
2000
.
Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres
.
Nat. Genet.
25
:
347
352
.
Zhu
Q.
,
Yasumoto
H.
,
Tsai
R.Y.
.
2006
.
Nucleostemin delays cellular senescence and negatively regulates TRF1 protein stability
.
Mol. Cell. Biol.
26
:
9279
9290
.
Zhu
Q.
,
Meng
L.
,
Hsu
J.K.
,
Lin
T.
,
Teishima
J.
,
Tsai
R.Y.
.
2009
.
GNL3L stabilizes the TRF1 complex and promotes mitotic transition
.
J. Cell Biol.
185
:
827
839
.

    Abbreviations used in this paper:
     
  • ALT

    alternative lengthening of telomeres

  •  
  • APB

    ALT-associated PML body

  •  
  • coIP

    coimmunoprecipitation

  •  
  • GNL3L

    guanine nucleotide-binding protein-like 3-like

  •  
  • HR

    homologous recombination

  •  
  • IDF

    interstitial damage foci

  •  
  • NS

    nucleostemin

  •  
  • PML

    promyelocytic leukemia

  •  
  • TA+

    telomerase active

  •  
  • TIF

    telomere dysfunction-induced foci

  •  
  • TRF

    telomeric repeat-binding factor

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