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Cover Image
Cover Image
On the cover
Pigino et al. use cryoelectron tomography to obtain the three-dimensional structure of radial spokes (red), regulatory complexes that project from the outer microtubule doublets (grey) of cilia and flagella. Also shown in this reconstruction of a Chlamydomonas flagellum are the outer and inner dynein arms (light and dark blue), the dynein light and intermediate chains (yellow), and the dynein regulatory complex (green). Image courtesy of Pigino et al.
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In This Issue
In Focus
Setting a new standard for kinetochores
Two studies reassess the number of proteins at yeast kinetochores and centromeres.
People & Ideas
Denise Montell: Lighting the way in border cell migration
Montell studies Drosophila oogenesis, focusing primarily on the collective migration of border cells.
Review
Report
The Rap1–Rgl–Ral signaling network regulates neuroblast cortical polarity and spindle orientation
The Rap1–Rgl–Ral signaling network modulates asymmetric Drosophila neuroblast division in cooperation with other intrinsic polarity cues.
CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast
Current models of centromere/kinetochore architecture are not sufficient to explain the number of molecules of histone H3 variant CENP-A observed in quantitative microscopy.
Point centromeres contain more than a single centromere-specific Cse4 (CENP-A) nucleosome
Quantitative measurement of the number of Cse4, CBF3, and Ndc80 proteins at kinetochores reveals a 2.5–3-fold increased copy number relative to prior estimates.
Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation
Phosphorylation of the kinetochore component Nsk1 by Cdk1 antagonizes its localization to and function at the kinetochore and spindle during early mitosis.
Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis
Regulated RhoA localization at the cleavage site is mediated by the Sticky/Citron kinase and ensures RhoA’s proper activation and appropriate contractile ring dynamics during cytokinesis.
Article
An intramolecular salt bridge drives the soluble domain of GTP-bound atlastin into the postfusion conformation
Before ER tubule fusion, the atlastin GTPase undergoes a “prefusion” to “postfusion” conformational change that is mediated by an intramolecular salt bridge.
Hsp42 is required for sequestration of protein aggregates into deposition sites in Saccharomyces cerevisiae
The budding yeast heat shock protein Hsp42 coaggregates with misfolded proteins and may link those aggregates to further sorting factors.
Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex
The yeast class V myosin Myo4p moves processively in vivo in a cargo-dependent manner following formation of a double-headed complex with the adapter protein She3p and the mRNA-binding protein She2p.
Tim50’s presequence receptor domain is essential for signal driven transport across the TIM23 complex
The Tim50 subunit of the mitochondrial TIM23 complex contains a presequence-binding domain that is essential for viability and precursor transport across the inner membrane.
Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
Much like mammalian cells, yeast contain a Rho-dependent pathway for endocytosis in addition to canonical clathrin-dependent endocytosis.
Cryoelectron tomography of radial spokes in cilia and flagella
Cryo-EM tomography of wild-type and mutant cilia and flagella from Tetrahymena and Chlamydomonas reveals new information on the substructure of radial spokes.
Astrocyte pVHL and HIF-α isoforms are required for embryonic-to-adult vascular transition in the eye
The von Hippel–Lindau tumor suppressor and the hypoxia-inducible factor-αs are essential for the transition from embryonic hyaloidal vascular system to adult retinal vasculature in the mouse eye.
