-
Cover Image
Cover Image
On the cover
A time-lapse series of a Drosophila S2 cell lacking the PI(4)P 5-kinase Skittles reveals excessive blebbing of the plasma membrane (green) during mitosis. The absence of Skittles reduces PI(4,5)P2 levels, resulting in the mislocalization of the ERM protein Moesin and decreased cortical stability. The mitotic spindle is labeled by α-tubulin–mCherry (red). Image courtesy of Chantal Roubinet.
See page 99. - PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
In This Issue
In Focus
Why a protein switches sides during translation
Sequences within growing transmembrane proteins determine when loops reverse direction to cross the ER membrane.
People & Ideas
Howard Chang: Gene regulation in time and space
Chang studies how large groups of genes work together to carry out biological processes.
Review
Pathogens and polymers: Microbe–host interactions illuminate the cytoskeleton
Report
Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
Actin dynamics are required for proper cilia spacing, global coordination of cilia polarity, and coordination of metachronic cilia beating, whereas cytoplasmic microtubule dynamics are required for local coordination of polarity between neighboring cilia.
Article
Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor
A nuclear pool of partially spliced Clk1/4 pre-mRNAs matures in response to stress and induces SR protein phosphorylation and activation.
Transmembrane segments of nascent polytopic membrane proteins control cytosol/ER targeting during membrane integration
Vastly different folded transmembrane segments of nascent multispanning membrane proteins each induce structural changes in the ribosome tunnel and translocon that target the loops of the growing polypeptide alternately into the cytosol or ER lumen.
Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon
Interaction between L17 in the ribosome tunnel and folded nascent chain transmembrane segments during multi-spanning membrane protein synthesis triggers structural rearrangements in the ribosome that cause switching between cytosolic and ER lumenal targeting of the growing polypeptide.
Interaction between FIP5 and SNX18 regulates epithelial lumen formation
The Rab11 GTPase-binding protein FIP5 collaborates with the sorting nexin 18 to transport proteins to the apical surface and to tubulate membranes during epithelial apical lumen formation.
TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle
TPX2 promotes mitotic spindle formation by enhancing Eg5 accumulation on microtubules and limiting motor activity.
Molecular networks linked by Moesin drive remodeling of the cell cortex during mitosis
During mitosis, cortical Moesin activity is restricted to promote cell elongation and cytokinesis, but localized Moesin recruitment is necessary for polar bleb retraction and cortical relaxation.
FAK is required for the assembly of podosome rosettes
FAK opposes Rho activity and vimentin intermediate filament formation to promote podosome rosette assembly.
EMILIN1–α4/α9 integrin interaction inhibits dermal fibroblast and keratinocyte proliferation
The α4/α9 integrins directly engage the ECM glycoprotein EMILIN1 to inhibit skin cell proliferation upstream of TGF-β signaling.
Abcg2 labels multiple cell types in skeletal muscle and participates in muscle regeneration
Abcg2-expressing cells proliferate after muscle injury and are required for effective regeneration of multiple muscle cell lineages.
