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Cover Image
Cover Image
On the cover
Carbonaro et al. demonstrate that HIF-1α mRNA accumulates in P-bodies (green) when microtubules (magenta) are depolymerized, resulting in the transcription factor's translational repression. Image courtesy of Marisa Carbonaro.
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In This Issue
In Focus
Two microscopes are better than one
Researchers reveal way to sharpen vision of double-microscope technique.
People & Ideas
Sean Munro: Revealing the Golgi's true identity
Munro studies how proteins localize within the secretory pathway.
Review
Report
p62 targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding
p62 is recruited to the ER at an early-stage autophagosome formation independently of most Atg proteins.
Article
Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload
Components of the minichromosome maintenance complex (Mcm2-7) remain indefinitely bound to chromatin during G1 phase and replication arrest.
SET8 is degraded via PCNA-coupled CRL4(CDT2) ubiquitylation in S phase and after UV irradiation
Degradation of the histone H4 methyltransferase SET8, which regulates chromosome compaction and genomic integrity, is regulated by the CRL4(CDT2) ubiquitin ligase to facilitate DNA replication and repair.
Homeostatic levels of SRC-2 and SRC-3 promote early human adipogenesis
Individual or joint knockdown of the transcriptional coactivators SRC-2 and SRC-3 inhibits lipid accumulation in adipocytes by decreasing PPARγ activity.
IGF-II is regulated by microRNA-125b in skeletal myogenesis
miR-125b is identified as a myogenic miRNA that regulates skeletal muscle differentiation by targeting IGF-II under the control of mTOR signaling.
Microtubule disruption targets HIF-1α mRNA to cytoplasmic P-bodies for translational repression
Active translation of HIF-1α requires association and trafficking of HIF mRNA on dynamic microtubules, whereas microtubule disruption represses HIF-1α translation by releasing its mRNA from polysomes and sequestering it to P-bodies.
Dynamin2- and endothelial nitric oxide synthase–regulated invasion of bladder epithelial cells by uropathogenic Escherichia coli
eNOS-mediated S-nitrosylation of dynamin2 promotes infection of epithelial cells by E. coli.
Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision
New methodology improves the spatial resolution and sensitivity of correlative light and EM tomography, revealing new insights into dynamic cellular processes.
A role for oxysterol-binding protein–related protein 5 in endosomal cholesterol trafficking
ORP5 works together with Niemann Pick C-1 to facilitate exit of cholesterol from endosomes and lysosomes.
A novel fast mechanism for GPCR-mediated signal transduction—control of neurotransmitter release
In addition to calcium influx, charge movement in the G protein–coupled M2-muscarinic receptor is required for the control of acetylcholine release.
Heparan sulfate proteoglycan syndecan-3 is a novel receptor for GDNF, neurturin, and artemin
Syndecan-3 may act alone or as a coreceptor with RET to promote cell spreading, neurite outgrowth, and migration of cortical neurons by GNDF, NRTN, and ARTN.
BMP promotes motility and represses growth of smooth muscle cells by activation of tandem Wnt pathways
Vascular smooth muscle cell motility relies on interdependent activation of canonical and noncanonical Wnt signal transduction pathways; fibronectin, produced in response to BMP-2–mediated activation of β-catenin, promotes motility by activating an integrin-linked kinase via α4-integrin.
Su(dx) E3 ubiquitin ligase–dependent and –independent functions of Polychaetoid, the Drosophila ZO-1 homologue
Polychaetoid coordinates receptor trafficking and signaling with adherens junction organization.
