ERK1/2 MAP kinases promote cell cycle entry by rapid, kinase-independent disruption of retinoblastoma–lamin A complexes

ERK2 does not affect other lamin A and Rb interactions. (A) ERK2 does not affect LAP2α–Rb interaction. NIH3T3 cells were transfected with 1 μg each of vector (−), HA-ERK2-NLS-wt (wt), and HA-ERK2-DK (DK), grown until confluence, and kept in 0.5% CS for 18 h. Cellular lysates were immunoprecipitated with an antibody against LAP2α or with preimmune serum (PI), and immunoprecipitates (IP) and total lysates (TL) were probed for the indicated proteins (α protein of interest).