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Cover Image
Cover Image
On the cover
A mitotic cell shows incorrect attachments between spindle microtubules (green) and kinetochores (red) when the Aurora B kinase, which normally eliminates misattachments, is inhibited. Liu et al. reveal that protein phosphatase I is recruited to kinetochores to oppose Aurora B and stabilize correct microtubule binding.
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In This Issue
In Focus
Actin oligomers hit the assembly line
Study identifies a new way for actin filaments to polymerize in vivo.
People & Ideas
Review
Report
Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway
Enhanced polymerization of actin in latrunculin A–treated aip1Δ cells shows that filament assembly does not occur from monomeric actin alone in vivo.
Article
Mammalian BLM helicase is critical for integrating multiple pathways of meiotic recombination
Improper chromosome pairing, synapsis, and segregation impair meiotic progression in the absence of the BLM helicase in mammalian cells.
Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes
hMis14 and HP1 depend on each other to localize to the kinetochore and inner centromere, respectively.
Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase
KNL targets PP1 to kinetochores, where it antagonizes Aurora B activity.
ERO1-β, a pancreas-specific disulfide oxidase, promotes insulin biogenesis and glucose homeostasis
ERO1-β has an unexpected and selective function for oxidative protein folding in insulin-producing cells.
The TWEAK–Fn14 system is a critical regulator of denervation-induced skeletal muscle atrophy in mice
The TNF-related cytokine TWEAK promotes skeletal muscle atrophy that is associated with classical disuse syndromes.
Endogenous Bak inhibitors Mcl-1 and Bcl-xL: differential impact on TRAIL resistance in Bax-deficient carcinoma
Although both Mcl-1 and Bcl-xL keep proapoptotic Bak in check, it is the loss of Mcl-1 that sensitizes cells to death receptor–mediated apoptosis.
The PI3K p110α isoform regulates endothelial adherens junctions via Pyk2 and Rac1
Only the p110α isoform of PI3K mediates the association of VE-cadherin with Pyk2, a Rac GEF and the p85 PI3K regulatory subunit, to reduce junctional integrity in response to TNF.
Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation
FAK-mediated myosin-dependent paxillin phosphorylation is necessary to bring vinculin to maturing focal adhesions, reinforcing the link between the cytoskeleton and the ECM.
Focal adhesions are sites of integrin extension
FRET experiments model integrin conformation changes in adherent cells.
The Arf tumor suppressor protein inhibits Miz1 to suppress cell adhesion and induce apoptosis
Arf assembles a complex containing Miz1, heterochromatin, and histone H3K3 to block expression of genes involved in cell adhesion and signal transduction. The resulting blockade of cell–cell and cell–matrix interactions facilitates elimination of cells carrying oncogenic mutations.
Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis
Clathrin-coated pit size and dynamic behavior varies with low density lipoprotein receptor (LDLR) expression levels in a manner dependent on the LDLR-specific adaptors, Dab2 and ARH.
Fibroblast growth factor receptors 1 and 2 in keratinocytes control the epidermal barrier and cutaneous homeostasis
Loss of FGFRs results in skin abnormalities due to activation of keratinocytes and epidermal T cells.
Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans
The small GTPase Arl13b regulates ciliary transmembrane protein localizations and anterograde IFT assembly stability.
