Monoclonal antibody anti-4C8 blocks transendothelial migration of T cells at the intercellular borders of HUVECs. Human T cells were cocultured with a HUVEC monolayer under conditions that would normally promote transendothelial migration of T cells. Inclusion of anti-4C8 selectively blocks the transmigration step. The result, shown by scanning electron microscopy, is that T cells adhere firmly to the monolayer surface, flatten on it, and extend pseudopods into the junctions, but are unable to migrate further. See related article in this issue by Masuyama et al., pp. 979–989.
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Characterization of the 4C8 Antigen Involved in Transendothelial Migration of CD26hi T Cells after Tight Adhesion to Human Umbilical Vein Endothelial Cell Monolayers
Dramatic Rise in Plasma Viremia after CD8+ T Cell Depletion in Simian Immunodeficiency Virus–infected Macaques
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