Zhuang et al. introduce land-ExM, a superresolution approach that simultaneously maps protein and lipid ultrastructure in cells with high contrast. This method visualizes 3D interactions between membrane-bound organelles and phase-separated condensates, uncovering organelle contact sites such as stress granules at nuclear tunnels adjacent to nucleoli.

DMIM (also called MTSS1) promotes Rac1-mediated branched actin network formation and endocytosis to drive rapid, cyclical plasma membrane remodeling during embryonic syncytial divisions. Loss of DMIM leads to decreased branched actin networks and a coincident increase in bundled actin networks induced by RhoGEF2 and diaphanous.

Collado-Diaz et al., show that lymphatic endothelial cells generate a soluble chemokine variant, CCL21-ΔC, through uPA/plasmin-dependent cleavage. This process reshapes chemokine gradients in skin and LNs, thereby regulating dendritic cell migration and positioning and uncovering a proteolytic mechanism that fine-tunes immune surveillance.

Jackson and Heilmann et al. use a new apical polarity reporter mouse and high-resolution live imaging to demonstrate that pancreatic tubulogenesis is driven by dynamic transformations of existing lumens, which establish and remodel the pancreatic duct. The authors also demonstrate that lumen rearrangement always precedes outer branching and provides unique niches for endocrinogenesis.

Yang et al. show that C. elegans CKS-1 is required, with CDK-1, for anaphase B during oocyte meiosis I; promotes anaphase onset through securin and cyclin B1 destruction in meiosis I and II, respectively; and acts positively with both securin and cyclin B3 in meiosis II.

The authors show that epithelial and mesenchymal cells navigate chemical cues via fundamentally different force-generating mechanisms. Whereas epithelial groups rely on forces produced at internal junctions, mesenchymal clusters depend on coordinated supracellular contractions, offering new insight into how diverse cell types achieve collective movement during development and disease.

The focal adhesion protein vinculin affects the dynamics of the actin cytoskeleton, and reorganization of the actin cytoskeleton is an energetically demanding process important in cell migration. Here, Fabiano et al. establish a relationship between vinculin, cell bioenergetics, and migration behavior, and the RhoA/ROCK/myosin II pathway in MDA-MB-231 cells.