Lysosomal cargo sorting from the early endosomes should involve mechanisms that prevent their recycling to the plasma membrane. Here, Chouhan P and Phogat Y et al. report that the small GTP-binding protein Arl8b recruits the Rab11a GAP, TBC1D9B, to inactivate Rab11a-mediated recycling of newly synthesized LAMP1 and mediate its efficient sorting to lysosomes.
Zhou Chen, Dongqing Li, Nan Pang, Xiaomin Fang, Marco Gottardo, Guanglian Jiang, Nana Yuan, Jiawen Zhang, Tianling Li, Yuxuan Yan, Jinghan Li, Mingyu Pan, Jiaji Jiao, Maria Giovanna Riparbelli, Giuliano Callaini, Hélène Rangone, Jingyan Fu
Centriole microtubule architecture varies across species and developmental contexts. Chen et al. identify Ana1 as a regulator of centriolar doublet-to-triplet conversion and show that triplet microtubule integrity is crucial for sperm structure and male fertility.
Ziyan Wang, Bradley M. Bartholomai, Bin Wang, David Ritz, Daniel Schultz, Jennifer J. Loros, Jay C. Dunlap
Wang et al. develop a high-resolution, long-term live-cell imaging platform in Neurospora crassa to visualize circadian clock proteins expressed at endogenous levels. They show distinct dynamic WCC and FRQ nuclear bodies, FRQ oscillations synchronized among nuclei, and internuclear protein exchange, revealing how subcellular organization and internuclear communication coordinate timing in a multinucleated syncytium.
Lan Yang, Mikael Björklund, Cong Yi, Shue Chen, Zhi Hong
Yang et al. develop a light-based method to precisely modulate astrocytic Ca2+ signals via endogenous ER-IP3R pathways. Their approach reveals how local and global Ca2+ patterns differentially regulate organelle transport and astrocyte process growth, providing a versatile platform for dissecting Ca2+-dependent cell biology.
Wendi Williams, Kien Phan, Jing Chen, Stefan Legewie, Julia Kamenz, Silke Hauf
Chromosome segregation is triggered by separase and is abrupt and largely synchronous. Williams et al. show that synchronous chromosome segregation does not require separase-mediated positive feedback and that slight asynchrony may be unavoidable due to stochastic effects when only a few cohesin complexes remain at the time of sister chromatid separation.
Tatiana V. Petrova, Kelly de Korodi, Thea Berg, Tania Wyss, Yahya Mohammadzadeh, Lida Safazada, Kathleen Shah, Nicola L. Harris, Jeremiah Bernier-Latmani
Eosinophils are associated with infection and pathological fibrosis. However, healthy small intestinal villi harbor dense populations of these granulocytes. Here, Petrova et al. show that eosinophils convert PDGFRβ+ fibroblasts to specialized villus smooth muscle cells, driving a developmental program for postnatal intestinal remodeling and maturation.
Idaira M. Guerrero-Fonseca, Karina B. Hernández-Almaraz, Iliana I. León-Vega, Régis Joulia, Armando Montoya-García, Hilda Vargas-Robles, Theresia E.B. Stradal, Klemens Rottner, Reyna Oregon, Eduardo Vadillo, Jennifer L. Johnson, William B. Kiosses, Sergio D. Catz, Sussan Nourshargh, Michael Schnoor
Guerrero-Fonseca et al. provide evidence for a cellular cross talk through which neutrophils transfer proteases into endothelial cells to degrade cortactin, thus facilitating breaching of the vascular endothelium during inflammation.
Jiarui Jiang and Katheryn E. Rothenberg highlight work from Zheng and colleagues that elucidates how adhesion proteins are rapidly moved along the cell edge to vertices in response to mechanical forces.
Zubia-Aranburu et al. review multiscale forces governing T-cell function, their disruption in disease, and how mechanobiology can improve immunotherapy design.
