Shen et al. demonstrate that LMNA reduction alters peripheral genome organization in human cardiomyocytes through cytoskeletal forces transmitted by the LINC complex. These findings uncover a microtubule-dependent pathway regulating lamina-associated domains and suggest that the cytoskeleton may contribute to early nuclear dysfunction in laminopathies.

Agarwal et al. define the stage-specific gene expression programs of a leader cell that drives collective tissue invasion during organ development. By combining transcriptomics with functional analysis, they identify membrane trafficking as a central regulator of leader cell behavior, providing a valuable resource for studying collective cell migration in vivo.

Deb Roy et al. introduce an optogenetic tool to rapidly induce microtubule acetylation in living cells. The study shows that microtubule acetylation directly triggers release of the RhoGEF GEF-H1 from microtubules, activating actomyosin contractility, promoting focal adhesion maturation, and driving persistent directional cell migration.

Stalder and Pereira et al. combine affinity isolation of post-Golgi carriers, mass spectrometry, and a pooled CRISPR-KO screen to uncover new regulators of Golgi-to-plasma membrane transport. They show that a PTPN23-dependent ESCRT pathway is essential for the secretion of membrane and soluble cargoes, including hormones and antibodies, in specialized cells.

Rogers et al. demonstrate that heat stress triggers nucleolar remodeling in filamentous fungi, enabling segregation of damaged material and selective inheritance of a new nucleolar compartment. This reveals a chaperone-mediated quality control mechanism that preserves nuclear function in highly polarized, multinucleate cells.

Restrepo Cruz et al. employ super-resolution microscopy and Bayesian analyses to study the molecular interactions between tetraspanin CD82 and EGFR on the plasma membrane. They reveal the tetraspanin scaffold regulates the nanoscale organization of the receptor, modulating receptor multimerization and inhibiting ligand-independent receptor phosphorylation upstream of cellular proliferation.

Maintaining lipid bilayer integrity of membrane-bound organelles is essential for their ongoing and compartmentalized biochemical reactions. Kim et al. describe a mechanism for lysosomes to sense and respond to acute increases in their membrane tension by relieving hydrostatic pressure via the outward transport of major osmoticants.