Luan et al. identify the cullin-3 adaptor SHKBP1 as a noncanonical regulator of p62 phase behavior that limits p62 body formation, restrains Keap1 sequestration, and tunes Nrf2-dependent antioxidant responses to oxidative stress.

Contacts between ER and LDs not only facilitate lipid exchange but also serve as platforms for crucial subcellular processes. Viruses exploit subcellular organelles for their infection. Li et al. reveal that the NRZ tethering complex is a critical regulator of virus-induced ER–LD interactions by enhancing ER–LD contacts, thereby facilitating efficient viral infection.

Hamrick and Rog define the organization of the sister chromatids during meiosis in the nematode Caenorhabditis elegans. Meiotic exchanges are formed in the context of four DNA molecules: two identical sisters, each from the two parental chromosomes. Here, the sisters are found to occupy distinct volumes when exchanges form.

Zych et al. demonstrate that reduced RCC1 levels in micronuclei impair protein export, causing persistent micronuclear growth, nuclear lamina gap expansion, and rupture. In small micronuclei, chromatin state–dependent RCC1 depletion further disrupts nuclear import, inhibiting growth and micronucleus rupture.

Ambrosio et al. discover GRIPAP1 is a component of the cellular machinery that produces the platelet α-granule, a member of the lysosome-related organelle family. GRIPAP1 is an elongated homodimer functioning as a tethering factor at recycling endosome subdomains that mediate traffic of both biosynthetic and endocytosed proteins to the α-granule.

Falk et al. identify a mechanism coupling mitochondrial movement to energy demand. When energy levels are low, the energy-sensing kinase AMPK phosphorylates the motor/adaptor protein TRAK1, arresting mitochondria via the actin cytoskeleton. This mechanism likely helps to position mitochondria where they are most needed.

Schematics typically depict the actomyosin cortex below flat PM, but cells have complex PM topographies. Over the early Drosophila embryo, a composite of actomyosin and PM folds conveys cortical tension, and regulation involves PM topography modulation by Arp2/3.