George Galea, Karolina Kuodyte, Muzamil Majid Khan, Peter J. Thul, Beate Neumann, Emma Lundberg, Rainer Pepperkok
The Golgi is a well-established cytoplasmic hub, yet its coordination with the nucleus remains poorly understood. Systematic analysis of Golgi–nuclear proteins uncovers a DNA repair network, where RAD51C is anchored by giantin until DNA damage triggers ATM-dependent release and nuclear import, establishing the Golgi as a spatiotemporal regulator of nuclear DNA repair.
Chandini Bhaskar Naidu, Javier Vera Lillo, Eugenia Almacellas, Anabel-Lise Le Roux, Sabine Bardin, Nicolas Mateos, Jessica Angulo-Capel, Adam Wolowczyk, Yugo Terashima, Yuichi Wakana, Pere Roca-Cusachs, Maria F. Garcia-Parajo, Franck Perez, Bruno Goud, Jean-Baptiste Manneville, Stéphanie Miserey, Felix Campelo
Bhaskar Naidu and Vera Lillo et al. show that extracellular mechanical forces reciprocally regulate Golgi function. By linking cell adhesion and spreading, Golgi membrane mechanics, microtubule acetylation, lipid and protein kinase D signaling, and transport carrier formation, the study reveals a feedback pathway by which Golgi export supports cell spreading and mechanoadaptation.
Vera Janssen, Hannah de Kraker, Jesse S. Aaron, Annett de Haan, Iris de Heer, Satya Khuon, Jason Da Silva, Amber J.M. Driessen, Teng-Leong Chew, Josephine M.E. Tan, Anne K. Lagendijk, Ana Angulo-Urarte, Stephan Huveneers
Janssen et al. show that nanoscale membrane curvatures at asymmetric endothelial cell–cell junctions serve as a scaffold to recruit BAR proteins, including BIN1 and SNX9, during collective migration. These curvature-sensitive proteins coordinate junction remodeling, cell polarity, and directional movement, identifying membrane topology as a key organizer of endothelial guidance during vascular development.
Kaspar Gierke, Michalina Gadomska, Julia Breuer, Julius N. Bahr, Tanja M. Müller, Hanna Ehnis, Sina Zobel, Nancy Mejia Villagran, Sonja A. Kirsch, Alexandra Skrzypek, Renato Frischknecht, Anna Fejtová, Rainer A. Böckmann, Carolin Wichmann, Hanna Regus-Leidig, Johann Helmut Brandstätter
How SRs tether hundreds of synaptic vesicles to sustain continuous neurotransmitter release at sensory synapses remains unknown. In the current study, Gierke et al. demonstrate that the SR-associated protein Piccolino is essential for this tethering and propose a direct protein–lipid interaction as the underlying molecular mechanism.
Mariyah Saiduddin, Juhee Pae, Asier M. Vidal, Martin L. Alani, Ruth Lehmann
Saiduddin et al. use optogenetics and live imaging in Drosophila embryos to identify an unexpected role of Torso receptor tyrosine kinase in activating PI3K signaling. They demonstrate that the germplasm-specific ubiquitin ligase adaptor GCL is required to prune PIP3 from the membrane to establish the soma-germline boundary.
Mori et al. demonstrate that the presynaptic active zone proteins CAST and ELKS directly interact with endophilin-A to maintain the functional synaptic vesicle pool. This study redefines their roles beyond structural organization, highlighting how they coordinate synaptic vesicle release processes necessary for efficient neurotransmission.
Babli Adhikary, Alexander Chang, Atsuko Y. Higashi, Hideki Chiba, Ann L. Miller, Tomohito Higashi
Adhikary et al. show that PAK4 drives dynamic vertex remodeling in cells and embryos through spatiotemporal regulation of myosin II. Inhibition of PAK4 disrupts tissue packing and compromises junctional continuity and barrier function, indicating that PAK4 is essential for vertex remodeling to preserve tissue integrity.
Ruth M. Kearney, Anushka Sharma, Ciaran G. Morrison
Kearney, Sharma, and Morrison preview work from the Firat-Karalar lab, which reveals how PCM1 multimerization drives assembly of centriolar satellites and recruitment of client proteins.
Patricia L. Opresko and Orlando D. Schärer highlight work from Muniesa-Vargas and colleagues, which reveals insight into the mechanisms that regulate the dynamics of XPG recruitment and release during nucleotide excision repair.
Mami Sato, Florencio Porto Freitas, José Pedro Friedmann Angeli
José Pedro Friedmann Angeli and colleagues preview work from Ndoci et al., which reveals a system by which mitochondria prioritize CoQ retention when the mevalonate pathway is impaired, preserving respiration at the expense of antioxidant defenses.
