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Cover Image
Cover Image
On the cover
Transmission electron microscopy shows the presence of late-stage autophagic vacuoles in the neurites of dorsal root ganglion neurons subjected to three hours of starvation. These vacuoles may be amphisomes formed by the fusion of autophagosomes with late endosomes, a process that allows autophagic vacuoles to acquire the dynein motors they need to undergo retrograde transport and deliver their contents to lysosomes in the neuronal cell body.
Image © 2015 Cheng et al.
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In This Issue
In Focus
SUN protection for the skin
A component of the nuclear envelope–spanning LINC complex maintains the integrity of epidermal cell adhesions.
People & Ideas
Petra Schwille: Taking a minimalist approach to membranes
Schwille’s group breaks down cell phenomena into their simplest parts.
Feature
Review
Report
Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis
The Nek5 protein kinase contributes not only to uncoupling of the centrosome linker but also to integrity of the pericentriolar material and centrosomal microtubule nucleation, which together ensure the timely separation of centrosomes during early mitosis.
Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression
Hsp72 is a novel mitotic substrate of Nek6, and, together, these proteins play an essential role in assembly of robust mitotic spindles capable of efficient chromosome congression through K-fiber recruitment of the ch-TOG and TACC3 complex.
Head-to-tail regulation is critical for the in vivo function of myosin V
Myo2p is regulated by a head-to-tail interaction and defects in this regulatory mechanism lead to a host of cellular problems in vivo, including impaired cargo capture and delayed motor recycling
Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1
Rac1 promotes the rapid and dynamic localization of Formin-like 2 (FMNL2) to adherens junctions, where it promotes actin assembly.
Axonal autophagosomes recruit dynein for retrograde transport through fusion with late endosomes
Late endosome-loaded dynein–snapin complexes drive amphisome retrograde transport upon fusion of autophagosomes with late endosomes in distal axons.
Article
Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly
During anaphase B, Imp1-mediated transport of the AAA-ATPase Cdc48 protein at the membrane domain surrounding the mitotic spindle midzone promotes spindle midzone dissolution in fission yeast.
Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions
The LINC complex component SUN2 contributes to the mechanical integrity of intercellular adhesions between mammalian epidermal keratinocytes.
Tau reduction prevents Aβ-induced axonal transport deficits by blocking activation of GSK3β
Tau ablation, knockdown, and reconstitution studies in primary mouse neurons show that tau enables amyloid β oligomers to inhibit axonal transport through activation of GSK3β and through functions of tau that do not depend on its microtubule binding activity.
Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate
Rab27a controls the recruitment of phosphatidylinositol 4-kinase type 2α from endosomes to the plasma membrane, which promotes high levels of PI(4)P, fuels PI(4,5)P2 production, and favors the recruitment of Pr55Gag and HIV-1 assembly.
IL-1α induces thrombopoiesis through megakaryocyte rupture in response to acute platelet needs
An alternative pathway triggering enhanced platelet release from bone marrow megakaryocytes via a rupture-based mechanism is regulated by IL-1α in response to acute platelet requirements.
