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Cover Image
Cover Image
Close ModalOn the cover
Stramer et al. image the actin filaments (green) and microtubules (purple) of Drosophila macrophages as they migrate in vivo. When two cells collide, their cytoskeletons briefly align before contact repulsion drives the cells apart.
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In This Issue
In Focus
Kinetochores hang on for the ride
Two studies reveal how kinetochores stay attached to dynamic microtubules.
People & Ideas
Andy Dillin: Using aging research to probe biology
Dillin uses genetics to probe the pathways that control cellular and organismal longevity.
Review
Report
Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair
Cdc14A and Cdc14B knockout cells with double-strand breaks still arrest in G2, but they fail to repair the damage.
The Dam1 complex confers microtubule plus end–tracking activity to the Ndc80 kinetochore complex
The Ndc80 complex of kinetochores doesn't recognize microtubule tips by itself but relies on the Dam1 complex to track microtubule plus ends (see also related paper by Tien et al. in this issue).
Aurora A contributes to p150glued phosphorylation and function during mitosis
Phosphorylation of a dynactin subunit is important for its release from mitotic spindles.
Tuba, a Cdc42 GEF, is required for polarized spindle orientation during epithelial cyst formation
An RNAi screen picks Tuba out of the GTPase exchange factor (GEF) orchestra as a regulator of cell polarity in epithelial morphogenesis. (See also a companion paper from Rodriguez-Fraticelli et al., in this issue.)
Disease-causing mutations in Parkin impair mitochondrial ubiquitination, aggregation, and HDAC6-dependent mitophagy
Parkin catalyzes mitochondrial ubiquitination, recruiting autophagic components that clear damaged mitochondria. Defects in this pathway are implicated in Parkinson's disease.
Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo
A microtubule arm regulates cell–cell repulsion, pointing hemocytes in opposite directions when they contact each other in Drosophila embryos.
Botulinum hemagglutinin disrupts the intercellular epithelial barrier by directly binding E-cadherin
Botulinum neurotoxin's nontoxic HA protein binds E-cadherin to disrupt cell–cell adhesion in a species-specific manner.
Article
Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage
The Sox10 transcription factor is required to maintain as well as specify glial identity, adding new causes for the neuropathies associated with SOX10 mutations.
Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B
The Dam1 complex, regulated by aurora B phosphorylation, confers a more stable microtubule association for the Ndc80 complex at kinetochores (see also related paper by Lampert et al. in this issue).
The Cdc42 GEF Intersectin 2 controls mitotic spindle orientation to form the lumen during epithelial morphogenesis
Intersectin 2 localizes to centrosomes, where it regulates Cdc42 and helps to orient the apical surface correctly during cyst formation. (See also companion paper from Qin et al., in this issue.)
Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions
QUBIC, a specific and highly sensitive method for detection of protein–protein interactions, is used to identify new partners for the mitotic spindle components pericentrin and TACC3.
Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae
The myosin Myo4 is nonprocessive by itself, but when several motor subunits team up with She2 and She3, it forms an active RNP transport unit that moves ASH1 mRNA to the bud tip in dividing yeast.
