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Correction| November 26 2012

The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport

Nia J. Bryant,
Nia J. Bryant
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David E. James
David E. James
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Nia J. Bryant, David E. James
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2012 The Rockefeller University Press
2012
J Cell Biol (2012) 199 (5): 863.
https://doi.org/10.1083/jcb.2002120781995c
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Corrected article The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport

Vol. 161 No. 4, May 19, 2003. Pages 691–696.

The authors noticed a duplication of data in Fig. 3 B between the panels representing subcellular fractionations of wild-type and vps21Δ cells. Given the time that has elapsed since the original date of publication, the original data could not be found to correct the figure, so a new version of Fig. 3 B from a recent repeat of the experiment has been provided below.

The html and pdf versions of this article have been corrected. The error remains only in the print version.

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2012
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Figure 3.
Figure 3. Vps45p loses membrane association upon inactivation of PP1. (A) Tlg1p-containing complexes were immunoprecipitated from cells harboring the glc7–10 mutation (NOzY23) grown at 25°C (25°C), or grown at 25°C and then incubated at 37°C for 10 min before cell lysate preparation (37°C). Immunoblot analysis was used to detect the amount of Tlg1p, Tlg2p, Vti1p, HA-tagged Snc2p, and Vps45p in the immunoprecipitated complexes. (B) wild-type (SF838–9D), sec18–1 (NOzY22), vps21D (SGY79), and glc7–10 (PAY704–1) cells were incubated at 37°C for 10 min before fractionation by differential centrifugation to yield a whole cell extract (WCE), a low-speed membrane pellet (P13), a high-speed membrane pellet (P100), and a soluble, cytosolic fraction (S100). The amount of Vps45p in each fraction was assessed using immunoblot analysis.
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Vps45p loses membrane association upon inactivation of PP1. (A) Tlg1p-containing complexes were immunoprecipitated from cells harboring the glc7–10 mutation (NOzY23) grown at 25°C (25°C), or grown at 25°C and then incubated at 37°C for 10 min before cell lysate preparation (37°C). Immunoblot analysis was used to detect the amount of Tlg1p, Tlg2p, Vti1p, HA-tagged Snc2p, and Vps45p in the immunoprecipitated complexes. (B) wild-type (SF838–9D), sec18–1 (NOzY22), vps21D (SGY79), and glc7–10 (PAY704–1) cells were incubated at 37°C for 10 min before fractionation by differential centrifugation to yield a whole cell extract (WCE), a low-speed membrane pellet (P13), a high-speed membrane pellet (P100), and a soluble, cytosolic fraction (S100). The amount of Vps45p in each fraction was assessed using immunoblot analysis.

Figure 3.
Figure 3. Vps45p loses membrane association upon inactivation of PP1. (A) Tlg1p-containing complexes were immunoprecipitated from cells harboring the glc7–10 mutation (NOzY23) grown at 25°C (25°C), or grown at 25°C and then incubated at 37°C for 10 min before cell lysate preparation (37°C). Immunoblot analysis was used to detect the amount of Tlg1p, Tlg2p, Vti1p, HA-tagged Snc2p, and Vps45p in the immunoprecipitated complexes. (B) wild-type (SF838–9D), sec18–1 (NOzY22), vps21D (SGY79), and glc7–10 (PAY704–1) cells were incubated at 37°C for 10 min before fractionation by differential centrifugation to yield a whole cell extract (WCE), a low-speed membrane pellet (P13), a high-speed membrane pellet (P100), and a soluble, cytosolic fraction (S100). The amount of Vps45p in each fraction was assessed using immunoblot analysis.
View large Download slide

Vps45p loses membrane association upon inactivation of PP1. (A) Tlg1p-containing complexes were immunoprecipitated from cells harboring the glc7–10 mutation (NOzY23) grown at 25°C (25°C), or grown at 25°C and then incubated at 37°C for 10 min before cell lysate preparation (37°C). Immunoblot analysis was used to detect the amount of Tlg1p, Tlg2p, Vti1p, HA-tagged Snc2p, and Vps45p in the immunoprecipitated complexes. (B) wild-type (SF838–9D), sec18–1 (NOzY22), vps21D (SGY79), and glc7–10 (PAY704–1) cells were incubated at 37°C for 10 min before fractionation by differential centrifugation to yield a whole cell extract (WCE), a low-speed membrane pellet (P13), a high-speed membrane pellet (P100), and a soluble, cytosolic fraction (S100). The amount of Vps45p in each fraction was assessed using immunoblot analysis.

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Volume 199, Issue 5
26 November 2012
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