Using green fluorescent protein probes and rapid acquisition of high-resolution fluorescence images, sister centromeres in budding yeast are found to be separated and oscillate between spindle poles before anaphase B spindle elongation. The rates of movement during these oscillations are similar to those of microtubule plus end dynamics. The degree of preanaphase separation varies widely, with infrequent centromere reassociations observed before anaphase. Centromeres are in a metaphase-like conformation, whereas chromosome arms are neither aligned nor separated before anaphase. Upon spindle elongation, centromere to pole movement (anaphase A) was synchronous for all centromeres and occurred coincident with or immediately after spindle pole separation (anaphase B). Chromatin proximal to the centromere is stretched poleward before and during anaphase onset. The stretched chromatin was observed to segregate to the spindle pole bodies at rates greater than centromere to pole movement, indicative of rapid elastic recoil between the chromosome arm and the centromere. These results indicate that the elastic properties of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms.
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19 March 2001
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March 19 2001
Budding Yeast Chromosome Structure and Dynamics during Mitosis
In Special Collection:
JCB65: Cell Division, Cell Cycle, and Polarity
Chad G. Pearson,
Chad G. Pearson
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
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Paul S. Maddox,
Paul S. Maddox
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
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E.D. Salmon,
E.D. Salmon
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
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Kerry Bloom
Kerry Bloom
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
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Chad G. Pearson
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Paul S. Maddox
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
E.D. Salmon
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Kerry Bloom
aDepartment of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Abbreviations used in this paper: CFP, cyan fluorescent protein; Cse4p, centromere-specific histone; GFP, green fluorescent protein; lacI, lac repressor; YFP, yellow fluorescent protein.
Received:
October 18 2000
Revision Requested:
January 23 2001
Accepted:
January 23 2001
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2001 The Rockefeller University Press
2001
The Rockefeller University Press
J Cell Biol (2001) 152 (6): 1255–1266.
Article history
Received:
October 18 2000
Revision Requested:
January 23 2001
Accepted:
January 23 2001
Citation
Chad G. Pearson, Paul S. Maddox, E.D. Salmon, Kerry Bloom; Budding Yeast Chromosome Structure and Dynamics during Mitosis. J Cell Biol 19 March 2001; 152 (6): 1255–1266. doi: https://doi.org/10.1083/jcb.152.6.1255
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