ILC2s enter the brain from peripheral circulation in a CXCR1-dependent manner. (A) Experimental design of tracing and RNA sequencing of transferred EGFP-ILC2s in vivo (created with https://BioRender.com). (B) Changes in EGFP-positive cells in ischemic areas at different time points after MCAO modeling and EGFP-ILC2s transfer. Scale bars, 200 µm. (C) Density map showing brain-wide distribution of EGFP-positive cells 5 days after MCAO. Brighter color indicates higher density. Scale bar, 500 µm. (D) EGFP-positive cell counts per view from day 1 to 14 (n = 3). (E) Heatmap showing averaged and normalized GSVA results of four groups of ILC2s. (F) Volcano plot of DEGs of ILC2s between groups (|log2FC| > 0.25, adjusted P < 0.05). (G and H) Quantification of CXCR1 expression on brain-infiltrating ILC2s at day 1 and 5 after MCAO and EGFP-ILC2s transfer (n = 3), analyzed by two-sided, unpaired Student’s t test. All data represent biological replicates from two independent experiments. *P = 0.0226. (I) ILC2s were cultured in the presence of SCH563705+RPMI or RPMI only for 6 h before transferring (created with https://BioRender.com). (J and K) Flow cytometry gating strategy and quantification of transferred ILC2s at day 5 after ischemic stroke (n = 5), analyzed by two-sided, unpaired Student’s t test. All data represent biological replicates from two independent experiments. **P = 0.0015. GSVA, Gene Set Variation Analysis.