Simulations of EAAT5 anion channel activity. (A) Example of EAAT5 I_A(glu) evoked by single-vesicle release events in a rod. (B) Reaction scheme for EAAT5 (modified from a model for EAAT2) (Kolen et al., 2020). (C) Colored traces show the average simulated EAAT5 anion channel activity in four rods following the release of a single vesicle. The black trace shows the average change in I_A(glu) (±SD) evoked by single-vesicle release events in 15 rods (7–72 events/rod). (D) EAAT5 anion channel activity evoked in four rods by simulating the simultaneous release of 10 vesicles. (E) Comparison of the decline in glutamate molecules following the simulated release of a single vesicle with (blue trace) and without (black trace) 3,000 EAAT5 in rod 2. (F) Graph of the decline in glutamate following the simultaneous release of 10 vesicles in rod 2 with open (red trace) or closed (black trace) exits at the mouth of the synapse. The decline in a closed sphere with the same volume as rod 2 is plotted for comparison (blue trace). All simulations are the average of 12 runs with different seed values.