Quantification of principal components and cluster enrichments between PD-1 ^Hi and PD-1 ^Lo CD8 ⁺ TILs in WT mice and PD-1 ^Hi and PD-1 ^KO CD8 ⁺ TILs in inducible PD-1 ^del. mice. (A) Violin plots showing principal component 1 scores for each mouse individually for both experiment (Exp) 1 and Exp 2 (M2+M3+M4 = WT TME, M1+M5+M6 = inducible PD-1^del TME), split up by PD-1 status (PD-1^Hi versus PD-1^Lo in the WT TME, and PD-1^KO versus PD-1^Hi in the inducible PD-1^del TME). Shown are the CDES and Mann–Whitney P value for each mouse. In the WT TME, M2: P = 0 and CDES = −1.91; M3: P = 5.9 × 10⁻⁴⁸ and CDES = −1.40; M4: P = 1.4 × 10⁻³⁴ and CDES = −1.69. In the inducible PD-1^del. TME, M1: P = 0.00089 and CDES = −0.13; M5: P = 0.075 and CDES = −0.09; M6: P = 0.00032 and CDES = −0.20. (B) Violin plots showing the scores for principal components 2–10. Violins are colored by genotype and PD-1 status according to the Legend in the bottom right corner. Indicated above each pair within a genotype (e.g., PD-1^Hi versus PD-1^Lo or PD-1^KO versus PD-1^Hi) is the CDES. (C–E) Heat maps showing the proportion of cells in each cluster between CD8⁺ TILs in inducible PD-1^del. (PD-1^KO and PD-1^Hi) and WT control (PD-1^Hi and PD-1^Lo) mice. Values in each box indicated the raw cell counts (C), P values (D), and phi coefficient (E). Color indicates the proportion of cells in each cluster, normalized by columns. P values were calculated using Fisher’s exact test. (A–E) includes all cells from Exp 1+2 (containing four mice in total per group) grouped based on the genotype (WT versus inducible PD-1^del mice).