Glycogen is a key energy substrate in excitable tissue, including in skeletal muscle fibers where it also contributes to local energy production. Transmission electron microscopy imaging has revealed the existence of a heterogenic subcellular distribution of three distinct glycogen pools in skeletal muscle, which are thought to reflect the requirements for local energy stores at the subcellular level. Here, we show that the three main energy-consuming ATPases in skeletal muscles (Ca2+, Na+,K+, and myosin ATPases) utilize different local pools of glycogen. These results clearly demonstrate compartmentalized glycogen metabolism and emphasize that spatially distinct pools of glycogen particles act as energy substrate for separated energy requiring processes, suggesting a new model for understanding glycogen metabolism in working muscles, muscle fatigue, and metabolic disorders. These observations suggest that the distinct glycogen pools can regulate the functional state of mammalian muscle cells and have important implications for the understanding of how the balance between ATP utilization and ATP production is regulated at the cellular level in general and in skeletal muscle fibers in particular.
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July 07 2022
Specific ATPases drive compartmentalized glycogen utilization in rat skeletal muscle
Joachim Nielsen
,
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
Correspondence to Joachim Nielsen: jnielsen@health.sdu.dk
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Peter Dubillot
,
Peter Dubillot
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
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Marie-Louise H. Stausholm,
Marie-Louise H. Stausholm
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
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Niels Ørtenblad
Niels Ørtenblad
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
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1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
Peter Dubillot
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
Marie-Louise H. Stausholm
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
Niels Ørtenblad
1
Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark
Correspondence to Joachim Nielsen: jnielsen@health.sdu.dk
Received:
January 31 2022
Accepted:
June 22 2022
Online Issn: 1540-7748
Print Issn: 0022-1295
Funding
Funder(s):
Ministry of Culture
- Award Id(s): TKIF2011-058
© 2022 Nielsen et al.
2022
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Gen Physiol (2022) 154 (9): e202113071.
Article history
Received:
January 31 2022
Accepted:
June 22 2022
Citation
Joachim Nielsen, Peter Dubillot, Marie-Louise H. Stausholm, Niels Ørtenblad; Specific ATPases drive compartmentalized glycogen utilization in rat skeletal muscle. J Gen Physiol 5 September 2022; 154 (9): e202113071. doi: https://doi.org/10.1085/jgp.202113071
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