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1-8 of 8
Osamu Ohara
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Journal Articles
Shigeru Tanaka, Akira Suto, Taro Iwamoto, Takahiro Kageyama, Tomohiro Tamachi, Hiroaki Takatori, Kotaro Suzuki, Koichi Hirose, Osamu Ohara, Véronique Lefebvre, Hiroshi Nakajima
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2018) 215 (10): 2509–2519.
Published: 06 September 2018
Abstract
Peripherally induced regulatory T (pT reg) cells play indispensable roles in regulating gut inflammation; however, the mechanism underling the differentiation of pT reg cells under inflammatory conditions remains largely unknown. Here, we show that the expression of Sox12, a member of SoxC family, is significantly induced in T reg cells in colitic mice. We also show that TCR–NFAT signaling induces Sox12 expression in CD4 + T cells. Although Sox12 is not required for the development of thymus-derived T reg (tT reg) cells, Sox12 is involved in the development of pT reg cells under inflammatory conditions in an adoptive transfer colitis model. Moreover, we found that enforced expression of Sox12 is sufficient to promote Foxp3 expression in CD4 + T cells even in the absence of TGF-β or IL-2 and that Sox12 binds to Foxp3 promoter and drives its transcription. These results suggest that TCR-NFAT signaling induces the development of pT reg cells in colitic mice partly through Sox12 induction.
Includes: Supplementary data
Journal Articles
Akiko Hashimoto-Tane, Machie Sakuma, Hiroshi Ike, Tadashi Yokosuka, Yayoi Kimura, Osamu Ohara, Takashi Saito
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2016) 213 (8): 1609–1625.
Published: 27 June 2016
Abstract
The immunological synapse (IS) formed at the interface between T cells and antigen-presenting cells represents a hallmark of initiation of acquired immunity. T cell activation is initiated at T cell receptor (TCR) microclusters (MCs), in which TCRs and signaling molecules assemble at the interface before IS formation. We found that each TCR-MC was transiently bordered by a ring structure made of integrin and focal adhesion molecules in the early phase of activation, which is similar in structure to the IS in microscale. The micro–adhesion ring is composed of LFA-1, focal adhesion molecules paxillin and Pyk2, and myosin II (MyoII) and is supported by F-actin core and MyoII activity through LFA-1 outside-in signals. The formation of the micro–adhesion ring was transient but especially sustained upon weak TCR stimulation to recruit linker for activation of T cells (LAT) and SLP76. Perturbation of the micro–adhesion ring induced impairment of TCR-MC development and resulted in impaired cellular signaling and cell functions. Thus, the synapse-like structure composed of the core TCR-MC and surrounding micro–adhesion ring is a critical structure for initial T cell activation through integrin outside-in signals.
Includes: Supplementary data
Journal Articles
Takeshi Inoue, Masahiro Morita, Atsushi Hijikata, Yoko Fukuda-Yuzawa, Shungo Adachi, Kyoichi Isono, Tomokatsu Ikawa, Hiroshi Kawamoto, Haruhiko Koseki, Tohru Natsume, Taro Fukao, Osamu Ohara, Tadashi Yamamoto, Tomohiro Kurosaki
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2015) 212 (9): 1465–1479.
Published: 03 August 2015
Abstract
The CCR4–NOT deadenylase complex plays crucial roles in mRNA decay and translational repression induced by poly(A) tail shortening. Although the in vitro activities of each component of this complex have been well characterized, its in vivo role in immune cells remains unclear. Here we show that mice lacking the CNOT3 subunit of this complex, specifically in B cells, have a developmental block at the pro- to pre–B cell transition. CNOT3 regulated generation of germline transcripts in the V H region of the immunoglobulin heavy chain ( Igh ) locus, compaction of the locus, and subsequent Igh gene rearrangement and destabilized tumor suppressor p53 mRNA. The developmental defect in the absence of CNOT3 could be partially rescued by ablation of p53 or introduction of a pre-rearranged Igh transgene. Thus, our data suggest that the CCR4–NOT complex regulates B cell differentiation by controlling Igh rearrangement and destabilizing p53 mRNA.
Includes: Supplementary data
Journal Articles
Shigeru Tanaka, Akira Suto, Taro Iwamoto, Daisuke Kashiwakuma, Shin-ichiro Kagami, Kotaro Suzuki, Hiroaki Takatori, Tomohiro Tamachi, Koichi Hirose, Atsushi Onodera, Junpei Suzuki, Osamu Ohara, Masakatsu Yamashita, Toshinori Nakayama, Hiroshi Nakajima
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2014) 211 (9): 1857–1874.
Published: 29 July 2014
Abstract
Stat3 signaling is essential for the induction of RORγt and subsequent Th17 cell differentiation. However, the downstream targets of Stat3 for RORγt expression remain largely unknown. We show here that a novel isoform of Sox5, named Sox5t, is induced in Th17 cells in a Stat3-dependent manner. In vivo, T cell–specific Sox5-deficient mice exhibit impaired Th17 cell differentiation and are resistant to experimental autoimmune encephalomyelitis and delayed-type hypersensitivity. Retrovirus-mediated induction of Sox5 together with c-Maf induces Th17 cell differentiation even in Stat3-deficient CD4 + T cells but not in RORγt-deficient CD4 + T cells, indicating that Sox5 and c-Maf induce Th17 cell differentiation as downstream effectors of Stat3 and as upstream inducers of RORγt. Moreover, Sox5 physically associates with c-Maf via the HMG domain of Sox5 and DNA-binding domain of c-Maf, and Sox5 together with c-Maf directly activates the promoter of RORγt in CD4 + T cells. Collectively, our results suggest that Sox5 and c-Maf cooperatively induce Th17 cell differentiation via the induction of RORγt as downstream targets of Stat3.
Includes: Supplementary data
Journal Articles
Tomohiro Kaji, Akiko Ishige, Masaki Hikida, Junko Taka, Atsushi Hijikata, Masato Kubo, Takeshi Nagashima, Yoshimasa Takahashi, Tomohiro Kurosaki, Mariko Okada, Osamu Ohara, Klaus Rajewsky, Toshitada Takemori
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2012) 209 (11): 2079–2097.
Published: 01 October 2012
Abstract
One component of memory in the antibody system is long-lived memory B cells selected for the expression of somatically mutated, high-affinity antibodies in the T cell–dependent germinal center (GC) reaction. A puzzling observation has been that the memory B cell compartment also contains cells expressing unmutated, low-affinity antibodies. Using conditional Bcl6 ablation, we demonstrate that these cells are generated through proliferative expansion early after immunization in a T cell–dependent but GC-independent manner. They soon become resting and long-lived and display a novel distinct gene expression signature which distinguishes memory B cells from other classes of B cells. GC-independent memory B cells are later joined by somatically mutated GC descendants at roughly equal proportions and these two types of memory cells efficiently generate adoptive secondary antibody responses. Deletion of T follicular helper (Tfh) cells significantly reduces the generation of mutated, but not unmutated, memory cells early on in the response. Thus, B cell memory is generated along two fundamentally distinct cellular differentiation pathways. One pathway is dedicated to the generation of high-affinity somatic antibody mutants, whereas the other preserves germ line antibody specificities and may prepare the organism for rapid responses to antigenic variants of the invading pathogen.
Includes: Supplementary data
Journal Articles
In Special Collection:
2019 Lasker Prize Collection
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2008) 205 (8): 1807–1817.
Published: 14 July 2008
Abstract
Morphologically and functionally distinct subpopulations of human memory B (B Mem ) cells are identifiable by either their expression of CD27 or Fc receptor–like 4 (FCRL4), an immunoglobulin domain containing a receptor with strong inhibitory potential. We have conducted comparative transcriptome and proteome analyses of FCRL4 + and FCRL4 − B Mem cells and found that these two subsets have very distinctive expression profiles for genes encoding transcription factors, cell-surface proteins, intracellular signaling molecules, and modifiers of the cell-cycle status. Among the differentially expressed transcription factors, runt-related transcription factor 1 (RUNX1) transcript levels were up-regulated in FCRL4 − cells, whereas RUNX2 transcripts were preferentially detected in FCRL4 + cells. In vitro evidence for FCRL4 promoter responsiveness and in vivo promoter occupancy suggested that RUNX transcription factors are involved in the generation of these B Mem cell subpopulations. A distinctive signature profile was defined for the FCRL4 + B Mem cells by their expression of CD11c, receptor activator for nuclear factor κB ligand, and FAS cell-surface proteins, in combination with increased levels of SOX5 , RUNX2 , DLL1 , and AICDA expression. We conclude that this recently identified subpopulation of B Mem cells, which normally resides in epithelial tissue-based niches, may serve a unique role in mucosal defense and, conversely, as a target for neoplastic transformation events.
Includes: Supplementary data
Journal Articles
Michishige Harada, Kumiko Magara-Koyanagi, Hiroshi Watarai, Yuko Nagata, Yasuyuki Ishii, Satoshi Kojo, Shigetoshi Horiguchi, Yoshitaka Okamoto, Toshinori Nakayama, Nobutaka Suzuki, Wen-Chen Yeh, Shizuo Akira, Hiroshi Kitamura, Osamu Ohara, Ken-ichiro Seino, Masaru Taniguchi
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2006) 203 (13): 2929–2937.
Published: 18 December 2006
Abstract
Epidemiological studies have suggested that the recent increase in the incidence and severity of immunoglobulin (Ig)E-mediated allergic disorders is inversely correlated with Mycobacterium bovis bacillus Calmette Guerin (BCG) vaccination; however, the underlying mechanisms remain uncertain. Here, we demonstrate that natural killer T (NKT) cells in mice and humans play a crucial role in the BCG-induced suppression of IgE responses. BCG-activated murine Vα14 NKT cells, but not conventional CD4 T cells, selectively express high levels of interleukin (IL)-21, which preferentially induces apoptosis in Bε cells. Signaling from the IL-21 receptor increases the formation of a complex between Bcl-2 and the proapoptotic molecule Bcl-2–modifying factor, resulting in Bε cell apoptosis. Similarly, BCG vaccination induces IL-21 expression by human peripheral blood mononuclear cells (PBMCs) in a partially NKT cell–dependent fashion. BCG-activated PBMCs significantly reduce IgE production by human B cells. These findings provide new insight into the therapeutic effect of BCG in allergic diseases.
Includes: Supplementary data
Journal Articles
Yohichi Yasunami, Satoshi Kojo, Hiroshi Kitamura, Atsushi Toyofuku, Masayuki Satoh, Masahiko Nakano, Kentaroh Nabeyama, Yoshiichiroh Nakamura, Nobuhide Matsuoka, Seiyo Ikeda, Masao Tanaka, Junko Ono, Naoki Nagata, Osamu Ohara, Masaru Taniguchi
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2005) 202 (7): 913–918.
Published: 26 September 2005
Abstract
Pancreatic islet transplantation is a highly promising approach for the treatment of insulin-dependent diabetes mellitus. However, the procedure remains experimental for several reasons, including its low efficiency caused by the early graft loss of transplanted islets. We demonstrate that Gr-1 + CD11b + cells generated by transplantation and their IFN- γ production triggered by V α 14 NKT cells are an essential component and a major cause of early graft loss of pancreatic islet transplants. Gr-1 + CD11b + cells from V α 14 NKT cell–deficient ( J α 281 − / − ) mice failed to produce IFN- γ , resulting in efficient islet graft acceptance. Early graft loss was successfully prevented through the repeated administration of α -galactosylceramide, a specific ligand for V α 14 NKT cells, resulting in dramatically reduced IFN- γ production by Gr-1 + CD11b + cells, as well as V α 14 NKT cells. Our study elucidates, for the first time, the crucial role of Gr-1 + CD11b + cells and the IFN- γ they produce in islet graft rejection and suggests a novel approach to improving transplantation efficiency through the modulation of V α 14 NKT cell function.