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1-4 of 4
Niroshana Anandasabapathy
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Journal Articles
Gaëlle Breton, Jaeyop Lee, Yu Jerry Zhou, Joseph J. Schreiber, Tibor Keler, Sarah Puhr, Niroshana Anandasabapathy, Sarah Schlesinger, Marina Caskey, Kang Liu, Michel C. Nussenzweig
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2015) 212 (3): 401–413.
Published: 16 February 2015
Abstract
Two subsets of conventional dendritic cells (cDCs) with distinct cell surface markers and functions exist in mouse and human. The two subsets of cDCs are specialized antigen-presenting cells that initiate T cell immunity and tolerance. In the mouse, a migratory cDC precursor (pre-CDC) originates from defined progenitors in the bone marrow (BM). Small numbers of short-lived pre-CDCs travel through the blood and replace cDCs in the peripheral organs, maintaining homeostasis of the highly dynamic cDC pool. However, the identity and distribution of the immediate precursor to human cDCs has not been defined. Using a tissue culture system that supports the development of human DCs, we identify a migratory precursor (hpre-CDC) that exists in human cord blood, BM, blood, and peripheral lymphoid organs. hpre-CDCs differ from premonocytes that are restricted to the BM. In contrast to earlier progenitors with greater developmental potential, the hpre-CDC is restricted to producing CD1c + and CD141 + Clec9a + cDCs. Studies in human volunteers demonstrate that hpre-CDCs are a dynamic population that increases in response to levels of circulating Flt3L.
Includes: Supplementary data
Journal Articles
Niroshana Anandasabapathy, Rachel Feder, Shamim Mollah, Sze-Wah Tse, Maria Paula Longhi, Saurabh Mehandru, Ines Matos, Cheolho Cheong, Darren Ruane, Lucas Brane, Angela Teixeira, Joseph Dobrin, Olga Mizenina, Chae Gyu Park, Matthew Meredith, Björn E. Clausen, Michel C. Nussenzweig, Ralph M. Steinman
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2014) 211 (9): 1875–1891.
Published: 18 August 2014
Abstract
DCs are critical for initiating immunity. The current paradigm in vaccine biology is that DCs migrating from peripheral tissue and classical lymphoid-resident DCs (cDCs) cooperate in the draining LNs to initiate priming and proliferation of T cells. Here, we observe subcutaneous immunity is Fms-like tyrosine kinase 3 ligand (Flt3L) dependent. Flt3L is rapidly secreted after immunization; Flt3 deletion reduces T cell responses by 50%. Flt3L enhances global T cell and humoral immunity as well as both the numbers and antigen capture capacity of migratory DCs (migDCs) and LN-resident cDCs. Surprisingly, however, we find immunity is controlled by cDCs and actively tempered in vivo by migDCs. Deletion of Langerin + DC or blockade of DC migration improves immunity. Consistent with an immune-regulatory role, transcriptomic analyses reveals different skin migDC subsets in both mouse and human cluster together, and share immune-suppressing gene expression and regulatory pathways. These data reveal that protective immunity to protein vaccines is controlled by Flt3L-dependent, LN-resident cDCs.
Includes: Supplementary data
Journal Articles
Niroshana Anandasabapathy, Gabriel D. Victora, Matthew Meredith, Rachel Feder, Baojun Dong, Courtney Kluger, Kaihui Yao, Michael L. Dustin, Michel C. Nussenzweig, Ralph M. Steinman, Kang Liu
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2011) 208 (8): 1695–1705.
Published: 25 July 2011
Abstract
Antigen-presenting cells in the disease-free brain have been identified primarily by expression of antigens such as CD11b, CD11c, and MHC II, which can be shared by dendritic cells (DCs), microglia, and monocytes. In this study, starting with the criterion of Flt3 (FMS-like receptor tyrosine kinase 3)-dependent development, we characterize the features of authentic DCs within the meninges and choroid plexus in healthy mouse brains. Analyses of morphology, gene expression, and antigen-presenting function established a close relationship between meningeal and choroid plexus DCs (m/chDCs) and spleen DCs. DCs in both sites shared an intrinsic requirement for Flt3 ligand. Microarrays revealed differences in expression of transcripts encoding surface molecules, transcription factors, pattern recognition receptors, and other genes in m/chDCs compared with monocytes and microglia. Migrating pre-DC progenitors from bone marrow gave rise to m/chDCs that had a 5–7-d half-life. In contrast to microglia, DCs actively present self-antigens and stimulate T cells. Therefore, the meninges and choroid plexus of a steady-state brain contain DCs that derive from local precursors and exhibit a differentiation and antigen-presenting program similar to spleen DCs and distinct from microglia.
Includes: Supplementary data
Journal Articles
Marcella Fassò, Niroshana Anandasabapathy, Frances Crawford, John Kappler, C. Garrison Fathman, William M. Ridgway
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2000) 192 (12): 1719–1730.
Published: 11 December 2000
Abstract
We recently described a novel way to isolate populations of antigen-reactive CD4 + T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS ® sorting by CD4 high expression. Phenotypic, FACS ® , functional, antibody inhibition, and major histocompatibility complex–peptide tetramer analyses, as well as T cell receptor Vβ sequence analyses, of the antigen-specific CD4 high T cell populations demonstrated that a diverse sperm whale myoglobin 110–121–reactive CD4 + T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4 + T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4 + T cell memory.