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1-9 of 9
Marc K. Jenkins
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Journal Articles
In Special Collection:
JEM Immunology Collection 2021
Jeong Hyun Lee, Joyce K. Hu, Erik Georgeson, Catherine Nakao, Bettina Groschel, Thamotharampillai Dileepan, Marc K. Jenkins, Gregory Seumois, Pandurangan Vijayanand, William R. Schief, Shane Crotty
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2020) 218 (2): e20201254.
Published: 23 December 2020
Abstract
Immunodominance to nonneutralizing epitopes is a roadblock in designing vaccines against several diseases of high interest. One hypothetical possibility is that limited CD4 T cell help to B cells in a normal germinal center (GC) response results in selective recruitment of abundant, immunodominant B cells. This is a central issue in HIV envelope glycoprotein (Env) vaccine designs, because precursors to broadly neutralizing epitopes are rare. Here, we sought to elucidate whether modulating the quantity of T cell help can influence recruitment and competition of broadly neutralizing antibody precursor B cells at a physiological precursor frequency in response to Env trimer immunization. To do so, two new Env-specific CD4 transgenic (Tg) T cell receptor (TCR) mouse lines were generated, carrying TCR pairs derived from Env-protein immunization. Our results suggest that CD4 T cell help quantitatively regulates early recruitment of rare B cells to GCs.
Includes: Supplementary data
Journal Articles
Jessica A. Kotov, Dmitri I. Kotov, Jonathan L. Linehan, Vivian J. Bardwell, Micah D. Gearhart, Marc K. Jenkins
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2019) 216 (6): 1450–1464.
Published: 03 May 2019
Abstract
CD4 + T helper 17 (Th17) cells protect vertebrate hosts from extracellular pathogens at mucosal surfaces. Th17 cells form from naive precursors when signals from the T cell antigen receptor (TCR) and certain cytokine receptors induce the expression of the RORγt transcription factor, which activates a set of Th17-specific genes. Using T cell–specific loss-of-function experiments, we find that two components of the Polycomb repressive complex 1.1 (PRC1.1), BCL6 corepressor (BCOR) and KDM2B, which helps target the complex to unmethylated CpG DNA islands, are required for optimal Th17 cell formation in mice after Streptococcus pyogenes infection. Genome-wide expression and BCOR chromatin immunoprecipitation studies revealed that BCOR directly represses Lef1 , Runx2 , and Dusp4 , whose products inhibit Th17 differentiation. Together, the results suggest that the PRC1.1 components BCOR and KDM2B work together to enhance Th17 cell formation by repressing Th17 fate suppressors.
Includes: Supplementary data
Journal Articles
Justin J. Taylor, Ryan J. Martinez, Philip J. Titcombe, Laura O. Barsness, Stephanie R. Thomas, Na Zhang, Shoshana D. Katzman, Marc K. Jenkins, Daniel L. Mueller
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2012) 209 (11): 2065–2077.
Published: 15 October 2012
Abstract
B cell tolerance to self-antigen is critical to preventing antibody-mediated autoimmunity. Previous work using B cell antigen receptor transgenic animals suggested that self-antigen–specific B cells are either deleted from the repertoire, enter a state of diminished function termed anergy, or are ignorant to the presence of self-antigen. These mechanisms have not been assessed in a normal polyclonal repertoire because of an inability to detect rare antigen-specific B cells. Using a novel detection and enrichment strategy to assess polyclonal self-antigen–specific B cells, we find no evidence of deletion or anergy of cells specific for antigen not bound to membrane, and tolerance to these types of antigens appears to be largely maintained by the absence of T cell help. In contrast, a combination of deleting cells expressing receptors with high affinity for antigen with anergy of the undeleted lower affinity cells maintains tolerance to ubiquitous membrane-bound self-antigens.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2012) 209 (3): 597–606.
Published: 27 February 2012
Abstract
Memory B cells can be produced from the classical germinal center (GC) pathway or a less understood GC-independent route. We used antigen-based cell enrichment to assess the relative contributions of these pathways to the polyclonal memory B cell pool. We identified a CD38 + GL7 + B cell precursor population that differentiated directly into IgM + or isotype-switched (sw) Ig + memory B cells in a GC-independent fashion in response to strong CD40 stimulation. Alternatively, CD38 + GL7 + B cell precursors had the potential to become Bcl-6 + GC cells that then generated primarily swIg + memory B cells. These results demonstrate that early IgM + and swIg + memory B cells are products of a GC-independent pathway, whereas later switched Ig + memory B cells are products of GC cells.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2006) 203 (4): 1045–1054.
Published: 27 March 2006
Abstract
We explored the relationship between the time of naive CD4 + T cell exposure to antigen in the primary immune response and the quality of the memory cells produced. Naive CD4 + T cells that migrated into the skin-draining lymph nodes after subcutaneous antigen injection accounted for about half of the antigen-specific population present at the peak of clonal expansion. These late-arriving T cells divided less and more retained the central–memory marker CD62L than the T cells that resided in the draining lymph nodes at the time of antigen injection. The fewer cell divisions were related to competition with resident T cells that expanded earlier in the response and a reduction in the number of dendritic cells displaying peptide–major histocompatibility complex (MHC) II complexes at later times after antigen injection. The progeny of late-arriving T cells possessed the phenotype of central–memory cells, and proliferated more extensively during the secondary response than the progeny of the resident T cells. The results suggest that late arrival into lymph nodes and exposure to antigen-presenting cells displaying lower numbers of peptide–MHC II complexes in the presence of competing T cells ensures that some antigen-specific CD4 + T cells divide less in the primary response and become central–memory cells.
Journal Articles
Kathryn A. Pape, Valerie Kouskoff, David Nemazee, H. Lucy Tang, Jason G. Cyster, Lina E. Tze, Keli L. Hippen, Timothy W. Behrens, Marc K. Jenkins
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2003) 197 (12): 1677–1687.
Published: 09 June 2003
Abstract
The life history of isotype-switched B cells is unclear, in part, because of an inability to detect rare antigen-specific B cells at early times during the immune response. To address this issue, a small population of B cells carrying targeted antibody transgenes capable of class switching was monitored in immunized mice. After contacting helper T cells, the first switched B cells appeared in follicles rather than in the red pulp, as was expected. Later, some of the switched B cells transiently occupied the red pulp and marginal zone, whereas others persisted in germinal centers (GCs). Antigen-experienced IgM B cells were rarely found in GCs, indicating that these cells switched rapidly after entering GCs or did not persist in this environment.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (2003) 197 (6): 751–762.
Published: 10 March 2003
Abstract
The migration of antigen-specific T cells to nonlymphoid tissues is thought to be important for the elimination of foreign antigens from the body. However, recent results showing the migration of activated T cells into many nonlymphoid tissues raised the possibility that antigen-specific T cells do not migrate preferentially to nonlymphoid tissues containing antigen. We addressed this question by tracking antigen-specific CD4 T cells in the whole body after a localized subcutaneous antigen injection. Antigen-specific CD4 T cells proliferated in the skin-draining lymph nodes and the cells that underwent the most cell divisions acquired the ability to bind to CD62P. As time passed, CD62P-binding antigen-specific CD4 T cells with interferon γ production potential accumulated preferentially at the site of antigen injection but only in recipients that expressed CD62E. Surprisingly, these T cells did not proliferate in the injection site despite showing evidence of more cell divisions than the T cells in the draining lymph nodes. The results suggest that the most divided effector CD4 T cells from the lymph nodes enter the site of antigen deposition via recognition of CD62E on blood vessels and are retained there in a nonproliferative state via recognition of peptide–major histocompatibility complex II molecules.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (1998) 187 (2): 225–236.
Published: 19 January 1998
Abstract
The adoptive transfer of naive CD4 + T cell receptor (TCR) transgenic T cells was used to investigate the mechanisms by which the adjuvant lipopolysaccharide (LPS) enhance T cell clonal expansion in vivo. Subcutaneous administration of soluble antigen (Ag) resulted in rapid and transient accumulation of the Ag-specific T cells in the draining lymph nodes (LNs), which was preceded by the production of interleukin (IL)-2. CD28-deficient, Ag-specific T cells produced only small amounts of IL-2 in response to soluble Ag and did not accumulate in the LN to the same extent as wild-type T cells. Injection of Ag and LPS, a natural immunological adjuvant, enhanced IL-2 production and LN accumulation of wild-type, Ag-specific T cells but had no significant effect on CD28-deficient, Ag-specific T cells. Therefore, CD28 is critical for Ag-driven IL-2 production and T cell proliferation in vivo, and is essential for the LPS-mediated enhancement of these events. However, enhancement of IL-2 production could not explain the LPS-dependent increase of T cell accumulation because IL-2–deficient, Ag-specific T cells accumulated to a greater extent in the LN than wild-type T cells in response to Ag plus LPS. These results indicate that adjuvants improve T cell proliferation in vivo via a CD28-dependent signal that can operate in the absence of IL-2.
Journal Articles
Journal:
Journal of Experimental Medicine
Journal of Experimental Medicine (1997) 185 (12): 2133–2141.
Published: 16 June 1997
Abstract
Although lymphoid dendritic cells (DC) are thought to play an essential role in T cell activation, the initial physical interaction between antigen-bearing DC and antigen-specific T cells has never been directly observed in vivo under conditions where the specificity of the responding T cells for the relevant antigen could be unambiguously assessed. We used confocal microscopy to track the in vivo location of fluorescent dye-labeled DC and naive TCR transgenic CD4 + T cells specific for an OVA peptide–I-A d complex after adoptive transfer into syngeneic recipients. DC that were not exposed to the OVA peptide, homed to the paracortical regions of the lymph nodes but did not interact with the OVA peptide-specific T cells. In contrast, the OVA peptide-specific T cells formed large clusters around paracortical DC that were pulsed in vitro with the OVA peptide before injection. Interactions were also observed between paracortical DC of the recipient and OVA peptide-specific T cells after administration of intact OVA. Injection of OVA peptide-pulsed DC caused the specific T cells to produce IL-2 in vivo, proliferate, and differentiate into effector cells capable of causing a delayed-type hypersensitivity reaction. Surprisingly, by 48 h after injection, OVA peptide-pulsed, but not unpulsed DC disappeared from the lymph nodes of mice that contained the transferred TCR transgenic population. These results demonstrate that antigen-bearing DC directly interact with naive antigen-specific T cells within the T cell–rich regions of lymph nodes. This interaction results in T cell activation and disappearance of the DC.